Photoexcited triclosan induced DNA damage and oxidative stress via p38 MAP kinase signaling involving type I radicals under sunlight/UVB exposure

Dubey, Divya; Srivastav, Ajeet K.; Singh, Jyoti; Chopra, Deepti; Qureshi, Saba; Kushwaha, Hari Narayan; Singh, Nivedita; Ray, Ratan Singh

doi:10.1016/j.ecoenv.2019.02.065

Cited by 31 publications

(18 citation statements)

References 64 publications

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“…Cutaneous phototoxicity caused by UV radiation is primarily due to cell cytotoxicity, intracellular ROS accumulation, and apoptosis in keratinocytes. Therefore, it is more reasonable to focus on the inhibition of cell cytotoxicity, intracellular ROS accumulation, and apoptosis in UVA-and UVB-irradiated keratinocytes [ 36 , 37 ]. As described in this study [ 10 , 38 ], the intervention with KC extracts on photo-cytotoxicity (UVA: 20 J/cm 2 , UVB: 50 mJ/cm 2 ) results showed that KC extracts significantly reduced the cell cytotoxicity, intracellular ROS accumulation and apoptosis.…”

Section: Discussionmentioning

confidence: 99%

A Comparative Study on Photo-Protective and Anti-Melanogenic Properties of Different Kadsura coccinea Extracts

Jeon

Kang

Park

et al. 2021

Plants

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Kadsura coccinea (KC), a beneficial plant for human health, has been used for centuries in China, Thailand, and Korea in folk medicine and food. There is evidence supporting the biological effects of highly bioactive ingredients in KC such as lignans, triterpenoids, flavonoids, phenolic acids, steroids, and amino acids. In this study, we aimed to explore the effects, functions, and mechanisms of the extracts from KC root (KCR), stem (KCS), leaf (KCL), and fruit (KCF) in UVA and UVB-irradiated keratinocytes and α-melanocyte stimulating hormone (α-MSH)-stimulated melanocytes. First, the total polyphenol and flavonoid contents of KCR, KCS, KCL, and KCF and their radical scavenging activities were investigated. These parameters were found to be in the following order: KCL > KCR > KCS > KCF. UVA and UVB-irradiated keratinocytes were treated with KCR, KCS, KCL, and KCF, and keratinocyte viability, LDH release, intracellular ROS production, and apoptosis were examined. Our results demonstrated that KC extracts improved keratinocyte viability and reduced LDH release, intracellular ROS production, and apoptosis in the presence UVA and UVB irradiation. The overall photoprotective activity of the KC extracts was confirmed in the following order: KCL > KCR > KCS > KCF. Moreover, KC extracts significantly decreased the intracellular melanin content and tyrosinase activity in α-MSH-stimulated melanocytes. Mechanistically, KC extracts reduced the protein and mRNA expression levels of tyrosinase, tyrosinase-related protein-1 (TRP-1), and tyrosinase-related protein-2 (TRP-2) in α-MSH-stimulated melanocytes. In addition, these extracts markedly downregulated myophthalmosis-related transcription factor expression and cAMP-related binding protein phosphorylation, which is upstream of the regulation of Tyrosinase, TRP-1, and TRP-2. The overall anti-melanogenic activity of the KC extracts was established in the following order. KCL > KCR > KCS > KCF. Overall, the KC extracts exert photoprotective and anti-melanogenic effects, providing a basis for developing potential skin-whitening and photoprotective agents.

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Section: Discussionmentioning

confidence: 99%

A Comparative Study on Photo-Protective and Anti-Melanogenic Properties of Different Kadsura coccinea Extracts

Jeon

Kang

Park

et al. 2021

Plants

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“…However, ROS can also induce cell damage via oxidative stress when their levels are elevated [ 13 , 14 , 34 , 35 , 36 ]. Previous studies showed that TCS treatment resulted in an increase in ROS levels in various cell types and organisms, including Escherichia coli K-12 [ 37 ], goldfish [ 38 ], rat PC12 cells [ 9 ], and human HaCat cells [ 39 ]. Consistent with the above reports, our results showed that ROS levels were significantly increased in embryos treated with TCS.…”

Section: Discussionmentioning

confidence: 99%

Effect of Triclosan Exposure on Developmental Competence in Parthenogenetic Porcine Embryo during Preimplantation

Kim

Park

Lee

et al. 2020

IJMS

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Triclosan (TCS) is included in various healthcare products because of its antimicrobial activity; therefore, many humans are exposed to TCS daily. While detrimental effects of TCS exposure have been reported in various species and cell types, the effects of TCS exposure on early embryonic development are largely unknown. The aim of this study was to determine if TCS exerts toxic effects during early embryonic development using porcine parthenogenetic embryos in vitro. Porcine parthenogenetic embryos were cultured in in vitro culture medium with 50 or 100 µM TCS for 6 days. Developmental parameters including cleavage and blastocyst formation rates, developmental kinetics, and the number of blastomeres were assessed. To determine the toxic effects of TCS, apoptosis, oxidative stress, and mitochondrial dysfunction were assessed. TCS exposure resulted in a significant decrease in 2-cell rate and blastocyst formation rate, as well as number of blastomeres, but not in the cleavage rate. TCS also increased the number of apoptotic blastomeres and the production of reactive oxygen species. Finally, TCS treatment resulted in a diffuse distribution of mitochondria and decreased the mitochondrial membrane potential. Our results showed that TCS exposure impaired porcine early embryonic development by inducing DNA damage, oxidative stress, and mitochondrial dysfunction.

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“…The clinical improvement, induced by NB‐UVB therapy, was reflected by a significant ( P < .0001) decrease in disease severity as evidenced by reduction in PASI of 30% and a significant ( P < .0001) improvement in quality of life, mirrored by a decrease of 29% in the DLQI. UV exposure and more specifically NB‐UVB is known to induce oxidative stress on skin cells 6,7,19 . On the other hand, psoriatic skin, as inflammatory skin disease, shows a decrease of the anti‐oxidative network capacity compared to healthy skin 1,20 .…”

Section: Discussionmentioning

confidence: 99%

“…Ultraviolet light induces apoptosis and enhances oxidative stress in the skin 6,7 . On the other hand, UV therapy, such as narrow‐band UVB (NB‐UVB) phototherapy in particular, leads to clinical improvement of psoriasis 8,9 .…”

Section: Introductionmentioning

confidence: 99%

Epidermal carotenoid levels in vivo of patients with plaque psoriasis: Effects of narrow‐band UVB phototherapy

Darlenski

Deliyska

Al-Sadek

et al. 2020

Photoderm Photoimm Photomed

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Background Psoriasis is a systemic inflammatory disease characterized by hindered antioxidant defense and increased formation of free radicals. There are limited data on the skin carotenoids in psoriatic skin as well as their modulation during narrow‐band UVB (NB‐UVB) phototherapy of the disease. Aim The aim of this prospective study is to reveal the skin carotenoids levels during NB‐UVB phototherapy of psoriasis in humans. Material and methods Twenty Caucasian subjects with mild‐to‐moderate plaque psoriasis (15m; 5f) were enrolled in the study, and nine gender‐ and age‐matched healthy volunteers were recruited for controls of oxidative stress measurements. All psoriasis patients underwent 10 sessions of NB‐UVB phototherapy. Measurements were taken at baseline and after 10 sessions of NB‐UVB phototherapy. The assessment of carotenoid levels in the skin in vivo was performed by a non‐invasive, reflectance spectroscopy‐based device. Psoriasis severity was assessed by psoriasis area and severity index (PASI). The dermatology life quality index (DLQI) was evaluated in psoriatic patients. Results Baseline carotenoid levels were significantly lower in psoriasis patients in comparison to healthy controls. NB‐UVB phototherapy insignificantly diminished carotenoid levels in the skin of psoriasis patients, while clinical improvement both in PASI score and DLQI was observed. Conclusion We showed the levels of skin carotenoids in psoriatic patients are lower than in healthy subjects. NB‐UVB did not change significantly skin carotenoid levels. Further studies should elucidate the potential effect of antioxidants supplementation during NB‐UVB of psoriasis.

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Photoexcited triclosan induced DNA damage and oxidative stress via p38 MAP kinase signaling involving type I radicals under sunlight/UVB exposure

Cited by 31 publications

References 64 publications

A Comparative Study on Photo-Protective and Anti-Melanogenic Properties of Different Kadsura coccinea Extracts

A Comparative Study on Photo-Protective and Anti-Melanogenic Properties of Different Kadsura coccinea Extracts

Effect of Triclosan Exposure on Developmental Competence in Parthenogenetic Porcine Embryo during Preimplantation

Epidermal carotenoid levels in vivo of patients with plaque psoriasis: Effects of narrow‐band UVB phototherapy

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