Photografting of 2-methacryloyloxyethyl phosphorylcholine from polydimethylsiloxane: Tunable protein repellency and lubrication property

Goda, Tatsuro; Matsuno, Ryosuke; Konno, Tomohiro; Takai, Madoka; Ishihara, Kazuhíko

doi:10.1016/j.colsurfb.2007.11.014

Cited by 55 publications

(40 citation statements)

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“…The graft thickness linearly increased with an increase in the UV irradiation time. 20,21 Surface properties of original substrates, including the surface contact angle and the surface f-potential, were significantly altered through modification with MPC polymers. For example, according to measurements of dynamic contact angles (DCAs) of water as shown in Table 2, various hydrophobic polymeric substrate surfaces were significantly changed to hydrophilic by simply dip coating in PMB (Fig.…”

Section: Interfacial Properties Of Phospholipid Polymer Biointerfacesmentioning

confidence: 99%

“…For surface modification of microchannels in labon-a-chip devices with MPC polymers, several strategies such as filling coating, 75,87,95,111,113 photo-induced polymerization, 20,21,94 and electrospray deposition (ESD), 68,71-73 have been developed. These are based on various molecular designs of MPC polymers aimed at different substrate materials and diverse applications.…”

Section: Molecular Design Of Phospholipid Polymers For Lab-on-a-chip mentioning

confidence: 99%

“…MPC polymers have been used to form cellmembrane-like interfaces for chip applications via physical adsorption, 4,68,70,73,75,76,87,95 chemical bonding, 22,25,29,38,[111][112][113] and photo-induced grafting. 20,21 In this paper, we have reviewed recent achievements and progress in the development of various functional MPC polymer biointerfaces for lab-on-a-chip devices and biomedical applications.…”

Section: Introductionmentioning

confidence: 99%

“…Fluorescence images of protein adsorptions in PMBSSi (0.30 mg mL21 ) modified (d, e, f) and unmodified (a, b, c) quartz microchannels. First, the microchannels were completely filled with 0.45 mg mL 21 of FITC-BSA solution (a, d) and then incubated for 1 h at 4°C.…”

mentioning

confidence: 98%

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Phospholipid Polymer Biointerfaces for Lab-on-a-Chip Devices

Takai

Ishihara

2010

Ann Biomed Eng

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This review summarizes recent achievements and progress in the development of various functional 2-methacryloyloxyethyl phosphorylcholine (MPC) polymer biointerfaces for lab-on-a-chip devices and applications. As phospholipid polymers, MPC polymers can form cell-membrane-like surfaces by surface chemistry and physics and thereby provide biointerfaces capable of suppressing protein adsorption and many subsequent biological responses. In order to enable application to microfluidic devices, a number of MPC polymers with diverse functions have been specially designed and synthesized by incorporating functional units such as charge and active ester for generating the microfluidic flow and conjugating biomolecules, respectively. Furthermore, these polymers were incorporated with silane or hydrophobic moiety to construct stable interfaces on various substrate materials such as glass, quartz, poly(methyl methacrylate), and poly(dimethylsiloxane), via a silane-coupling reaction or hydrophobic interactions. The basic interfacial properties of these interfaces have been characterized from multiple aspects of chemistry, physics, and biology, and the suppression of nonspecific bioadsorption and control of microfluidic flow have been successfully achieved using these biointerfaces on a chip. Further, many chip-based biomedical applications such as immunoassays and DNA separation have been accomplished by integrating these biointerfaces on a chip. Therefore, functional phospholipid polymer interfaces are promising and useful for application to lab-on-a-chip devices in biomedicine.

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Section: Interfacial Properties Of Phospholipid Polymer Biointerfacesmentioning

confidence: 99%

Section: Molecular Design Of Phospholipid Polymers For Lab-on-a-chip mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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confidence: 98%

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Phospholipid Polymer Biointerfaces for Lab-on-a-Chip Devices

Takai

Ishihara

2010

Ann Biomed Eng

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“…14,15 We have selected electron beam-induced graft polymerization (EIGP) in various surface modification methods of PDMS microchannels. [16][17][18][19] For PDMS surface modification, exposure to energies, such as oxygen plasma, 20 ultraviolet light, 21 corona discharges 22 are the well known methods to provide a hydrophilic PDMS surface. However, hydrophobicity recovery is a problem in view of long-term storage, and adding other functions is difficult, such as target molecule, protein, nucleic acid capturing.…”

Section: Introductionmentioning

confidence: 99%

Preparation of a Surface-functionalized Power-free PDMS Microchip for MicroRNA Detection Utilizing Electron Beam-induced Graft Polymerization

et al. 2017

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We propose an easy microchannel surface functionalization method for a poly(dimethylsiloxane) (PDMS) microchip that utilizes electron beam-induced graft polymerization (EIGP) as a platform for microchip-based biomarker analysis. Unlike other grafting techniques, EIGP enables rapid surface modification of PDMS without initiator immobilization. The grafted microchip is preservable, and can be easily functionalized for versatile applications. In this study, the surfacefunctionalized power-free microchip (SF-PF microchip) was used for the detection of microRNA (miRNA), which is a biomarker for many serious diseases. The EIGP enables high-density three-dimensional probe DNA immobilization, resulting in rapid and sensitive miRNA detection on the portable SF-PF microchip. The limit of detection was 0.8 pM, the required sample volume was 0.5 μL, and the analysis time was 15 min. The SF-PF microchip will be a versatile platform for microchip-based point-of-care diagnosis.

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