Photoinactivation of Salmonella enterica (serovar Typhimurium) by tetra-cationic porphyrins containing peripheral [Ru(bpy)2Cl]+ units

Gonçalves, Pablo José; Bezzerra, Fabio C.; Teles, Amanda Vargas; Menezes, Lucas B.; Alves, Kamilla M.; Alonso, Laís; Alonso, Antônio; Andrade, María Auxiliadora; Borissevitch, Iouri; Souza, Guilherme Rocha Lino de; Iglesias, Bernardo A.

doi:10.1016/j.jphotochem.2020.112375

Cited by 29 publications

(10 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In recent years, there has been increased interest in using the light activation of porphyrins to treat bacterial infections. This treatment is referred to as antimicrobial photodynamic therapy (aPDT), which is based on studies using the light activation of porphyrins to treat cancer via PDT [34][35][36][37][38][39][40][41][42][43][44]. Studies have shown the ability of cationic porphyrins to kill bacterial cells when photoactivated, via the production of reactive oxygen species (ROS); Gram-positive (Staphylococcus aureus) and Gram-negative (Escherichia coli) bacteria, as well as fungi (Candida albicans), are indeed susceptible to light-activated porphyrins [45][46][47][48][49].…”

Section: Introductionmentioning

confidence: 99%

A Cationic Porphyrin, ZnPor, Disassembles Pseudomonas aeruginosa Biofilm Matrix, Kills Cells Directly, and Enhances Antibiotic Activity of Tobramycin

2020

View full text Add to dashboard Cite

One of the greatest threats to human health is the rise in antibiotic-resistant bacterial infections. Pseudomonas aeruginosa (PsA) is an “opportunistic” pathogen known to cause life-threatening infections in immunocompromised individuals and is the most common pathogen in adults with cystic fibrosis (CF). We report here a cationic zinc (II) porphyrin, ZnPor, that effectively kills planktonic and biofilm-associated cells of PsA. In standard tests against 16–18 h-old biofilms, concentrations as low as 16 µg/mL resulted in the extensive disruption and detachment of the matrix. The pre-treatment of biofilms for 30 min with ZnPor at minimum inhibitory concentration (MIC) levels (4 µg/mL) substantially enhanced the ability of tobramycin (Tobra) to kill biofilm-associated cells. We demonstrate the rapid uptake and accumulation of ZnPor in planktonic cells even in dedicated heme-uptake system mutants (ΔPhu, ΔHas, and the double mutant). Furthermore, uptake was unaffected by the ionophore carbonyl cyanide m-chlorophenyl hydrazine (CCCP). Cells pre-exposed to ZnPor took up the cell-impermeant dye SYTOXTM Green in a concentration-dependent manner. The accumulation of ZnPor did not result in cell lysis, nor did the cells develop resistance. Taken together, these properties make ZnPor a promising candidate for treating multi-drug-resistant infections, including persistent, antibiotic-resistant biofilms.

show abstract

Section: Introductionmentioning

confidence: 99%

A Cationic Porphyrin, ZnPor, Disassembles Pseudomonas aeruginosa Biofilm Matrix, Kills Cells Directly, and Enhances Antibiotic Activity of Tobramycin

2020

View full text Add to dashboard Cite

show abstract

Section: Resultsmentioning

confidence: 99%

“…28 The similar values of Φ Δ for both porphyrin derivatives, 3 (Φ Δ = 0.65 ± 0.02) and 9 (Φ Δ = 0.51 ± 0.04), compared with TPP as a reference compound (Φ Δ = 0.52) indicated that the developed porphyrin derivatives 3 and 9 could have phototoxicity comparable to TPP. 33 In vitro phototoxicity assessment Upon light absorption, PSs can generate ROS which damage cellular components such as unsaturated lipids, amino acid residues, and nucleic acids, resulting in cell death. 34 Therefore, the in vitro phototoxicity of 3 and 9 was evaluated on Colon 26 cancer cells by a MTT assay with and without light irradiation.…”

Section: Singlet Oxygen Quantum Yieldmentioning

confidence: 99%

Synthesis and evaluation of radiolabeled porphyrin derivatives for cancer diagnoses and their nonradioactive counterparts for photodynamic therapy

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Gonçalves et al . developed a ruthenium and zinc bimetallic system, which upon activation by white light generates 1 O 2 as well as O 2 ⋅− and HO ⋅ to kill Salmonella enterica serotype typhimurium [81] . The complex consists of a porphyrin core appended with four Ru complexes.…”

Section: Effect Of the Metal Center On Apdtmentioning

confidence: 99%

Recent Advances in Metal Complexes for Antimicrobial Photodynamic Therapy

Rees

Hess

2023

ChemBioChem

View full text Add to dashboard Cite

Antimicrobial resistance (AMR) is a growing global problem with more than 1 million deaths due to AMR infection in 2019 alone. New and innovative therapeutics are required to overcome this challenge. Antimicrobial photodynamic therapy (aPDT) is a rapidly growing area of research poised to provide much needed help in the fight against AMR. aPDT works by administering a photosensitizer (PS) that is activated only when irradiated with light, allowing high spatiotemporal control and selectivity. The PS typically generates reactive oxygen species (ROS), which can damage a variety of key biological targets, potentially circumventing existing resistance mechanisms. Metal complexes are well known to display excellent optoelectronic properties, and recent focus has begun to shift towards their application in tackling microbial infections. Herein, we review the last five years of progress in the emerging field of small‐molecule metal complex PSs for aPDT.

show abstract

Photoinactivation of Salmonella enterica (serovar Typhimurium) by tetra-cationic porphyrins containing peripheral [Ru(bpy)2Cl]+ units

Cited by 29 publications

References 52 publications

A Cationic Porphyrin, ZnPor, Disassembles Pseudomonas aeruginosa Biofilm Matrix, Kills Cells Directly, and Enhances Antibiotic Activity of Tobramycin

A Cationic Porphyrin, ZnPor, Disassembles Pseudomonas aeruginosa Biofilm Matrix, Kills Cells Directly, and Enhances Antibiotic Activity of Tobramycin

Synthesis and evaluation of radiolabeled porphyrin derivatives for cancer diagnoses and their nonradioactive counterparts for photodynamic therapy

Recent Advances in Metal Complexes for Antimicrobial Photodynamic Therapy

Contact Info

Product

Resources

About