Photoinduced cleavage and DNA-binding of the Ruthenium(II) polypyridyl complex [Ru(phen)2(ipbd)](ClO4)2

Abstract

A novel ruthenium(II) polypyridyl complex [Ru(phen) 2 {ipbd)](ClO 4 ) 2 (ipbd = 3-(1H-Imidazo[4,5-f][1,10]phenanthrolin-2-yl)-1-benzodioxane, phen = 1,10-phenanthroline} has been synthesized and characterized by elemental analysis, ES-MS and 1 H-NMR spectra. The interaction of the complex with calf thymus DNA (CT-DNA) was investigated by absorption titration, fluorescence spectra, thermal denaturation, viscosity measurements, circular dichroism and photoinduced cleavage. The results suggest that the complex intercalates into the CT-DNA base pairs. Upon irradiation, this novel Ru(II) complex can cleave the plasmid pBR 322 DNA from the supercoiled form I to the open circular form II.

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