Photoinduced Electron Transfer-Based Fluorescence Quenching Combined with Rolling Circle Amplification for Sensitive Detection of MicroRNA

Zhou, Fulin; Rong, Meng; Liu, Qiang; Jin, Yan; Li, Baoxin

doi:10.1002/slct.201601485

Cited by 10 publications

(2 citation statements)

References 48 publications

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“…75 Zhou and co-workers reported a novel homogenous miRNA detection by using the quenching ability of guanine (Figure 1.19). 76 The circular DNA was used as a recognition probe for the hybridization with miRNA target, and then miRNA target primed an rolling circle amplification (RCA) reaction to generate a long dsDNA target with a tandem repeated sequence. The RCA product could be hybridized with thousands of FAM-labeled signal DNA probes to form double-stranded DNA (dsDNA), which induced the fluorophore to be positioned near the -GGG-base, leading to the quenching of the fluorophore through the PET mechanism.…”

Section: Guanine Rich Sequence Dna As a Quenchermentioning

confidence: 99%

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Interactions of pyrrolidinyl peptide nucleic acid with oligodeoxyguanosine and biosensing applications

Charoenpakdee

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Pyrrolidinyl peptide nucleic acid is a DNA mimic consisting of proline-2-aminocyclopentane carboxylic acid (acpcPNA) as a core structure. It binds to DNA to form a more stable hybrid while showing greater selectivity and affinity than the conventional DNA and PNA probes. In this study, we aim to develop novel DNA sensing systems based on the acpcPNA probe. In the first part, graphene oxide (GO) was used as an external quencher which could effectively quench the fluorescein-labeled PNA probe. A remarkable fluorescence restoration was achieved after the addition of a complementary DNA target. This acpcPNA-GO combination was successfully used to monitor the strand-invasion of the duplex DNA by acpcPNA, and to detect two DNA targets in a multiplex, multicolor detection. In the second part, we demonstrated the effective quenching of fluorescently labeled acpcPNA by using oligodeoxyguanosine as an external quencher. The electrostatic interaction between the positively-charged modification on the PNA probe and the phosphate backbone of the oligodeoxyguanosine (dGX) resulted in a strong association, resulting in an effective quenching of the fluorescence by photoinduced electron transfer. The addition of the complementary DNA strand rapidly and completely restored the fluorescence without detaching the dGX from the PNA probe. This sensing system could detect the complementary DNA target with single-base mismatch specificity and could be applied to detect various other DNA sequences. The acpcPNA-dGX platform has been used in the multiplex detection of two DNA sequences and to detect Hg?? ion as well as to monitor the PNA ligation. In the last part, a new DNA sensing system based on the combination of acpcPNA-dGX in the context of DNAzyme was explored. Preliminary studies revealed a promising result from the colorimetric DNA detection employing a strand displacement probe strategy. In short, binding of the dGX-modified DNA strand to PNA diminished its peroxidase activity, which could be restored when the DNA target was added to displace the DNAzyme.

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Section: Guanine Rich Sequence Dna As a Quenchermentioning

confidence: 99%

“…Figure 1 19. The strategy for PET-based RCA fluorescence detection of miRNA with linear DNA as signal probe 76. …”

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confidence: 99%

Interactions of pyrrolidinyl peptide nucleic acid with oligodeoxyguanosine and biosensing applications

Charoenpakdee

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Live‐Cell Imaging of MicroRNA Expression via Photoinduced Electron Transfer Controlled by Catalytic Hairpin Assembly

Na,

Koo,

Park

et al. 2024

Adv Healthcare Materials

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MicroRNAs (miRNAs) serve as emerging biomarkers for a range of diseases, and their quantitative analysis draws increasing attention. Yet, current invasive methods limit continuous tracking within living cells. To overcome this, a nonenzymatic DNA‐based nanoprobe is developed for dynamic, noninvasive miRNA tracking via live‐cell imaging. This probe features a unique hairpin DNA structure with five guanines that act as internal quenchers, suppressing fluorescence from an attached fluorophore via photoinduced electron transfer. Target miRNA initiates toehold‐mediated strand displacement, restoring, and amplifying the fluorescence signal. Additionally, by introducing a single mismatch to the hairpin DNA, the nanoprobe's sensitivity is significantly enhanced, lowering the detection limit to about 60 pM without compromising specificity. To optimize intracellular delivery for prolonged monitoring, the nanoprobe is encapsulated within multilamellar lipid nanovesicles, fluorescently labeled for dual‐wavelength ratiometric analysis. The proposed nanoprobe demonstrates a significant advance in live‐cell miRNA detection, promising enhanced in situ analysis for a better understanding of miRNAs’ pathophysiological function.

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Fluorescence Techniques Based on Nucleic Acid Amplification Strategies: Rational Design and Application

Song

Jiang

2019

Nucleic Acid Amplification Strategies for Biosensing, Bioimaging and Biomedicine

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Photoinduced Electron Transfer-Based Fluorescence Quenching Combined with Rolling Circle Amplification for Sensitive Detection of MicroRNA

Cited by 10 publications

References 48 publications

Interactions of pyrrolidinyl peptide nucleic acid with oligodeoxyguanosine and biosensing applications

Interactions of pyrrolidinyl peptide nucleic acid with oligodeoxyguanosine and biosensing applications

Live‐Cell Imaging of MicroRNA Expression via Photoinduced Electron Transfer Controlled by Catalytic Hairpin Assembly

Fluorescence Techniques Based on Nucleic Acid Amplification Strategies: Rational Design and Application

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