Photoinduced electron transfer in wild type and mutated flavodoxin from Desulfovibrio vulgaris, strain Miyazaki F.: Energy gap law

Lugsanangarm, Kiattisak; Pianwanit, Somsak; Kokpol, Sirirat; Tanaka, Fumio; Chosrowjan, Haik; Taniguchi, Seiji; Mataga, Noboru

doi:10.1016/j.jphotochem.2011.01.013

Cited by 30 publications

(34 citation statements)

References 56 publications

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“…3. The ultrafast experimental decays in several flavoprotein isoforms are satisfactorily reproduced with common PET parameters in the present method (Nunthaboot et al, 2008a(Nunthaboot et al, , 2010(Nunthaboot et al, , 2011Lugsanangarm et al, 2011c). 4.…”

mentioning

confidence: 77%

“…Fluorescence decay functions of four flavodoxin isoforms (WT, Y97F, W59F and DM) were simultaneously analyzed, with the atomic coordinates of these proteins obtained by MD simulation and KM theory, by Lugsanangarm et al (2011bLugsanangarm et al ( , 2011c. The PET parameters common among these flavodoxin systems are listed in Table 3.…”

Section: Pet Analysis With MD Snapshots Of Four Flavodoxin Isoformsmentioning

confidence: 99%

“…The PET parameters in the Table are common among the four isoforms of flavodoxin (WT, W59F, Y97F and DM), and were obtained according to the procedure described at Section 3.4. b For flavodoxins, the four isoforms (WT, Y97F, W59F, DM) were simultaneously analyzed. Data are taken from Lugsanangarm et al (2011bLugsanangarm et al ( , 2011c. c For the FMN binding proteins, the five isoforms (WT, E13T, E13Q, W32Y and W32A) were simultaneously analyzed.…”

Section: Pet Analysis With MD Snapshots Of Four Flavodoxin Isoformsmentioning

confidence: 99%

“…Mean physical quantities related to the PET in the Y97F and DM flavodoxin isoforms a . Lugsanangarm et al (2011c) …”

Section: Dynamics Of the Pet Rate And Related Physical Quantities In mentioning

confidence: 99%

“…We have been trying to quantitatively analyze PET in flavoproteins (Nunthaboot et al, 2008a(Nunthaboot et al, , 2008b(Nunthaboot et al, , 2009a(Nunthaboot et al, , 2009b(Nunthaboot et al, , 2010(Nunthaboot et al, , 2011Lugsanangarm et al, 2011bLugsanangarm et al, , 2011c, using the experimental and theoretical approaches of evaluating the ultrafast fluorescence dynamics of Iso in the flavoproteins and using MD simulation based approaches, respectively.The following conclusions have been derived on the mechanisms of PET in the flavoproteins.…”

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confidence: 99%

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Theoretical Analyses of Photoinduced Electron Transfer from Aromatic Amino Acids to the Excited Flavins in Some Flavoproteins

Lugsanangarm¹,

Nunthaboot²,

Pianwanit³

et al. 2012

Protein Structure

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confidence: 77%

Section: Pet Analysis With MD Snapshots Of Four Flavodoxin Isoformsmentioning

confidence: 99%

Section: Pet Analysis With MD Snapshots Of Four Flavodoxin Isoformsmentioning

confidence: 99%

“…Mean physical quantities related to the PET in the Y97F and DM flavodoxin isoforms a . Lugsanangarm et al (2011c) …”

Section: Dynamics Of the Pet Rate And Related Physical Quantities In mentioning

confidence: 99%

mentioning

confidence: 99%

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Theoretical Analyses of Photoinduced Electron Transfer from Aromatic Amino Acids to the Excited Flavins in Some Flavoproteins

Lugsanangarm¹,

Nunthaboot²,

Pianwanit³

et al. 2012

Protein Structure

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Investigating the spectral and electrochemical properties of novel flavin‐pyrene dyads separated via variable spacer

et al. 2022

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The present manuscript describes the synthesis and the photophysical properties of a pair of novel flavin-pyrene dyads where the donor and the acceptor entities are separated via variable spacer. The dyads were well characterized using standard techniques and investigated for their photophysical and electrochemical nature. The observed absorption spectra of the dyads mainly display peaks corresponding to the individual pyrene and flavin units, with some contribution from the flavin entity in the pyrene region. While, strong emission quenching was observed for both the dyads if compared to its individual constituents. However, a careful analysis of the emission spectra and the solvent dependent studies reveals subtle difference between the two dyads. While no significant difference could be observed when excited in the flavin region; excitation at the pyrene region displays a weak and broad emission band in case of closely connected dyad. Further, the electrochemical properties were investigated by cyclic voltammetry and the reduction ability was observed to follow the trend as FlPy2 < FlPy1 < Fl.

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Photoinduced Electron Transfer Modeling to Simulate Flavoprotein Fluorescence Decay

Nunthaboot

Lugsanangarm

Nueangaudom

et al. 2013

Methods in Molecular Biology

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A method of analysis is described on the photoinduced electron transfer (PET) from aromatic amino acids as tryptophans (Trp) and tyrosines (Tyr) to the excited isoalloxazine (Iso*) in FMN-binding proteins (FBP) from Desulfovibrio vulgaris (strain, Miyazaki F). Time-dependent geometrical factors as the donor-acceptor distances are determined by means of a molecular dynamics simulation (MDS) of the proteins. Fluorescence decays of the single mutated isoforms of FBP are used as experimental data. The electrostatic (ES) energy between the photoproducts and ionic groups in the proteins is introduced into the Kakitani and Mataga (KM) model, which is modeled for an electron transfer process in solution. The PET parameters contained in the KM rate are determined by means of a nonlinear least square method, according to the Marquardt algorithm. The agreement between the observed and calculated decays is quite good, but not optimal. Characteristics on PET in flavoproteins, obtained by the present method, are described. Possible improvements of the method are discussed.

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Photoinduced electron transfer in wild type and mutated flavodoxin from Desulfovibrio vulgaris, strain Miyazaki F.: Energy gap law

Cited by 30 publications

References 56 publications

Theoretical Analyses of Photoinduced Electron Transfer from Aromatic Amino Acids to the Excited Flavins in Some Flavoproteins

Theoretical Analyses of Photoinduced Electron Transfer from Aromatic Amino Acids to the Excited Flavins in Some Flavoproteins

Investigating the spectral and electrochemical properties of novel flavin‐pyrene dyads separated via variable spacer

Photoinduced Electron Transfer Modeling to Simulate Flavoprotein Fluorescence Decay

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