Photoinduced electron transfer mechanism between green fluorescent protein molecules and metal oxide nanoparticles

Acikgoz, Sabriye; Ulusu, Yakup; Akın, Seçkin; Sönmezoğlu, Savaş; Gökçe, İ̇sa; Incı, Mehmet Nacı

doi:10.1016/j.ceramint.2013.10.017

Cited by 21 publications

(19 citation statements)

References 43 publications

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“…For bi‐exponential fluorescence decay, the fluorescence intensity decays are recovered in terms of a multi‐exponential model

I_{λ, t} = true {true\sum}_{i = 1}^{2} f_{i} normalexp ((), - t true/ τ_{i})

…”

Section: Resultsmentioning

confidence: 99%

Spectroscopic study on the interaction of bovine serum albumin with zinc(II) phthalocyanine

Wang

et al. 2015

Luminescence

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The interaction between the photosensitive antitumour drug, 2(3),9(10),16(17),23(24)-tetra-(((2-aminoethylamino)methyl)phenoxy)phthalocyaninato-zinc(II) (ZnPc) and bovine serum albumin (BSA) has been investigated using various spectroscopic methods. This work may provide some useful information for understanding the interaction mechanism of anticancer drug-albumin binding and gain insight into the biological activity and metabolism of the drug in blood. Based on analysis of the fluorescence spectra, ZnPc could quench the intrinsic fluorescence of BSA and the quenching mechanism was static by forming a ground state complex. Meanwhile, the Stern-Volmer quenching constant (KSV), binding constant (Kb), number of binding sites (n) and thermodynamic parameters were obtained. Results showed that the interaction of ZnPc with BSA occurred spontaneously via hydrogen bond and van der Waal's force. According to Foster's non-radioactive energy transfer theory, the energy transfer from BSA to ZnPc occurred with high possibility. Synchronous fluorescence and circular dichroism (CD) spectra also demonstrated that ZnPc induced the secondary structure of and conformation changes in BSA, especially α helix.

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“…For bi‐exponential fluorescence decay, the fluorescence intensity decays are recovered in terms of a multi‐exponential model

I_{λ, t} = true {true\sum}_{i = 1}^{2} f_{i} normalexp ((), - t true/ τ_{i})

…”

Section: Resultsmentioning

confidence: 99%

Spectroscopic study on the interaction of bovine serum albumin with zinc(II) phthalocyanine

Wang

et al. 2015

Luminescence

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“…Powder XRD patterns exhibited the cubic phase of CdO [44][45][46][47]19,[48][49][50][51], and the average crystalline size was calculated. From the optical absorption spectrum, the crystal field and inter-electronic repulsion parameters were evaluated as Dq = 855, B = 810, and C = 3000 cm À1 .…”

Section: Resultsmentioning

confidence: 99%

Room temperature synthesis and spectral characterizations of Fe3+-doped CdO powder

Reddy

Shim

Byon

et al. 2014

Journal of Molecular Structure

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h i g h l i g h t sFe 3+ ion doped CdO powder was synthesized at room temperature. XRD data reveals the crystal structure and average crystalline size. Optical absorption spectrum exhibited characteristic bands for Fe 3+ ions. From the EPR spectrum spin Hamiltonian value was evaluated. FT-IR spectrum indicates the fundamental vibrations of host lattice and metal ion. a b s t r a c tFe 3+ doped CdO powder was prepared at room temperature using a mild and simple solution method. X-ray diffraction (XRD) analysis showed that the prepared material has cubic structure. The average crystalline size evaluated using the Debye-Scherrer method is 24 nm. Scanning electron microscope (SEM) images show the surface morphology of nano-crystalline CdO powder, and the Energy dispersive X-ray spectroscopy (EDX) spectrum indicates the presence of a transition metal ion. The optical absorption spectrum shows characteristic bands for Fe 3+ with octahedral site symmetry. The crystal field parameter (Dq) and the Racah inter-electronic repulsion parameters (B and C) were evaluated. The electron paramagnetic resonance (EPR) spectrum shows a resonance signal at g = 2.0128, which indicates Fe 3+ ions in tetragonally-distorted octahedral site symmetry. This is also confirmed using the optical absorption spectrum. FT-IR shows the fundamental vibrations of the host lattice.

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“…The fluorescence lifetime (τ) is expressed as τ 0 = 1/(Γ + Γ m + k nr ) where Γ is radiative decay, k nr is the non-radiative decay rate and subscript m denotes metal, while the quantum efficiency of a scintillator material in the presence of a nearby metal Q 0 is expressed as Q 0 = (Γ + Γ m )/(Γ + Γ M + k nr ) and it indicates the possibility to simultaneously decrease the decay time by 14% and increase the luminescence intensity by introducing Γm. Attachment of GFP molecules to TiO 2 and cadmium oxide (CdO) was investigated with time correlated single photon counting [201]. Time-resolved experiments show that the τ of GFP molecules bound to MONPs got shortened by 43% and 22% for TiO 2 and CdO, respectively, due to photoinduced electron transfer caused by the interaction of GFP molecules (donor) and MONPs (acceptor).…”

Section: Fluorescence Detection Of Transition Metal Oxide Nanoparticlesmentioning

confidence: 99%

Current State of Laser-Induced Fluorescence Spectroscopy for Designing Biochemical Sensors

Taylor

Lai

2021

Chemosensors

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Laser-induced fluorescence (LIF) has been a valuable analytical technique since the 1970s that has only been made more useful through advances in other scientific fields such as biochemistry. Moreover, advances in laser and detector technology have seen a decrease in LIF detector costs and an increase in their ease of use. These changes have allowed for LIF technology to be widely adopted for various sensor designs in combination with advanced instruments. With advances in biochemistry necessitating the detection of complex metabolites, labelling with fluorescent chemical reagents may be necessary to improve detection sensitivity. Furthermore, advances made in fluorescent labeling technologies have allowed for the use of LIF in the detection of nanoparticles as well as for imaging techniques using nanoparticles as signal amplifiers. This technology has become invaluable in the detection of environmental pollutants, monitoring of biological metabolites, biological imaging, and cancer diagnosis, making it one of the most valuable analytical science techniques currently available.

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Photoinduced electron transfer mechanism between green fluorescent protein molecules and metal oxide nanoparticles

Cited by 21 publications

References 43 publications

Spectroscopic study on the interaction of bovine serum albumin with zinc(II) phthalocyanine

Spectroscopic study on the interaction of bovine serum albumin with zinc(II) phthalocyanine

Room temperature synthesis and spectral characterizations of Fe3+-doped CdO powder

Current State of Laser-Induced Fluorescence Spectroscopy for Designing Biochemical Sensors

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