Photoinduced Regeneration of an Aptamer-Based Electrochemical Sensor for Sensitively Detecting Adenosine Triphosphate

Zhang, Xiaoyu; Song, Chunxia; Yang, Ke; Hong, Wenwen; Lu, Ying; Yu, Ping; Mao, Lanqun

doi:10.1021/acs.analchem.7b05442

Cited by 79 publications

(42 citation statements)

References 19 publications

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“…Single-stranded DNA was adsorbed on the surface of GO through π-π stacking, fluorescence resonance energy transfer (FRET) occurred between PicoGreen dye combined with singlestranded DNA and GO, and fluorescence was quenched. But double-stranded DNA with perfect pairing did not have this property (Zhang et al 2018a;Li et al 2011;Zhang et al 2018b;Zhang et al 2018c). Scheme 1 exhibits the principle of an aptasensor for ATP detection.…”

Section: Resultsmentioning

confidence: 99%

A label-free logic gate hairpin aptasensor for sensitive detection of ATP based on graphene oxide and PicoGreen dye

Zhang

et al. 2021

J Anal Sci Technol

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Background In this paper, a simple, enzyme-free, label-free fluorescence, high sensitivity logic gate hairpin aptasensor was developed for adenosine triphosphate (ATP) detection based on graphene oxide (GO) and PicoGreen dye. Methods Using single-strand deoxyribonucleic acid (DNA) and adenosine triphosphate (ATP) as input signal and fluorescence signal as output signal, if single-strand DNA (DNA-L), single-strand DNA (DNA-S), and ATP were present at the same time, one segment of DNA-L formed a hairpin ring with ATP, and the other segment of DNA-L formed a completely complementary hairpin stem with DNA-S. The hairpin DNA was detached from the GO surface, and PicoGreen dye was embedded into the hairpin stem, and the fluorescence signal was enhanced. The molecular logic gate was constructed through the establishment of logic histogram, logic circuit, truth table, and logic formula. The biosensor-related performances including sensitivity, selectivity, and linearity were investigated, respectively. Results We have successfully constructed a AND logic gate. The detection limit of ATP is 138.0 pmol/L (3σ/slope) with detection range of 50–500 nmol/L (R2 = 0.98951), and its sensitivity is 4.748 × 106–6.875 × 108 a.u. (mol/L)−1. Conclusions The logic gate hairpin aptamer sensor has the advantages of high sensitivity, low detection limit, and low cost, and can be successfully applied to the detection of adenosine triphosphate (ATP) in actual human urine samples.

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Section: Resultsmentioning

confidence: 99%

A label-free logic gate hairpin aptasensor for sensitive detection of ATP based on graphene oxide and PicoGreen dye

Zhang

et al. 2021

J Anal Sci Technol

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“…More recently, azobenzene was incorporated into an aptamer‐based electrochemical sensor ( Figure A). [ 45 ] An ATP aptamer was modified internally with azobenzene, with the 3' terminus modified with ferrocene. In the trans conformation, the aptamer hairpin was stabilized, bringing the ferrocene close to the sensor surface and generating an electrochemical signal.…”

Section: Photoinduced Aptamer Switchingmentioning

confidence: 99%

Section: Photoinduced Aptamer Switchingmentioning

confidence: 99%

Engineering Aptamer Switches for Multifunctional Stimulus‐Responsive Nanosystems

et al. 2020

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specific and strong recognition of molecular targets (i.e., aptamers), catalyze biochemical reactions (i.e., ribozymes), or form sophisticated and dynamic "smart materials" or even 3D constructs (i.e., DNA origami). [2] There is particular interest in the use of nucleic acids for the construction of molecular switches, which undergo a function-altering structural change in response to an external stimulus. [3] Many such examples exist in nature, where switches enable organisms to sense physiological changes and selectively control biological functions in response. For example, riboswitches exist naturally as domains within messenger RNA (mRNA) transcripts that can bind to specific metabolites with high specificity in order to stabilize a secondary structure that prevents subsequent translation. [4] Synthetic, engineered molecular switches based on nucleic acids can achieve even greater diversity of function. Such designs make use of aptamersnucleic acid-based affinity reagents isolated via an in vitro process of systematic evolution by exponential enrichment (SELEX), in which DNA or RNA sequences with specific ligand binding are isolated from a large pool of random sequences. [5] Aptamers have proven to be robust recognition elements due to their thermal stability, ease of synthesis, and capacity to undergo ready modification with a broad array of functional groups. Importantly, traditional selection methods can be expanded upon by innovative engineering techniques to enable the incorporation of additional functions that further extend the utility of aptamers. Aptamers can adopt various 2-and 3-D structures such as duplexes, hairpins, and G-quadruplexes, and the inducible formation and disruption of these configurations can be exploited to enable complex functions besides simple biosensing. Consequently, aptamers have been incorporated into many intricate configurations, such as logic gates and dynamic nanomachines. Various research groups have also described aptamer-based switches that undergo conformational changes in response to triggers such as shifts in pH, stimulation with light, or the presence of a specific ligand. [6] Although still a relatively young area of research, these efforts could prove highly useful for materials research-conferring even greater and more selective control over devices based on functionalized nucleic acids. Aptamers are becoming increasingly integrated with various organic and inorganic molecules in order to control the assembly and operation of novel functional Although RNA and DNA are best known for their capacity to encode biological information, it has become increasingly clear over the past few decades that these biomolecules are also capable of performing other complex functions, such as molecular recognition (e.g., aptamers) and catalysis (e.g., ribozymes). Building on these foundations, researchers have begun to exploit the predictable base-pairing properties of RNA and DNA in order to utilize nucleic acids as functional materials that can undergo a molecular "switching" ...

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“…Although electrical aptasensors offer advantages such as simplicity, low cost, good sensitivity and selectivity, at present, their use in clinical laboratories remains limited [29]. A comprehensive review about the recent progress and challenges in designing electrochemical aptasensors, their effectiveness in protein disease biomarkers or contaminants detection in food and the environment is reported in [30][31][32][33].…”

Section: Aptamers In Electrical Nanobiosensorsmentioning

confidence: 99%

Modelling and Development of Electrical Aptasensors: A Short Review

2018

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Aptamers are strands of DNA or RNA molecules, chemically synthetized and able to bind a wide range of targets, from small molecules to live cells, and even tissues, with high affinity and specificity. Due to their efficient targeting ability, they have many different kinds of applications. Particularly attractive is their use in biotechnology and disease therapy, in substitution of antibodies. They represent a promising way for early diagnosis (aptasensors), but also for delivering imaging agents and drugs and for inhibiting specific proteins (therapeutic aptamers). Starting by briefly reviewing the most recent literature concerning advances in biomedical applications of aptamers and aptasensors, the focus is on the issues of a theoretical/computational framework (proteotronics) for modelling the electrical properties of biomolecules. Some recent results of proteotronics concerning the electrical, topological and affinity properties of aptamers are reviewed.

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Photoinduced Regeneration of an Aptamer-Based Electrochemical Sensor for Sensitively Detecting Adenosine Triphosphate

Cited by 79 publications

References 19 publications

A label-free logic gate hairpin aptasensor for sensitive detection of ATP based on graphene oxide and PicoGreen dye

A label-free logic gate hairpin aptasensor for sensitive detection of ATP based on graphene oxide and PicoGreen dye

Engineering Aptamer Switches for Multifunctional Stimulus‐Responsive Nanosystems

Modelling and Development of Electrical Aptasensors: A Short Review

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