Photolithographic Boronate Affinity Molecular Imprinting: A General and Facile Approach for Glycoprotein Imprinting

Li, Li; Lu, Yue; Bie, Zijun; Chen, Hong‐Yuan; Liu, Zhen

doi:10.1002/anie.201207950

Cited by 238 publications

(166 citation statements)

References 38 publications

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“…The K a value between MINP (glucose) and glucose is 1.18 Â 10 3 L mol À1 , and the K a value of the other monosaccharides is negligible, which is in good agreement with the expected selective binding of the target compound. Molecular imprinting creates complementary objects that are increasingly popular, 154 and the number of binding sites of per nanoparticle can be controlled by the ratio of surfactant to the target compound. It is completely watersoluble and can be used to precisely position and orient the binding groups on a cross-linked micelle, which distinguishes different carbohydrates.…”

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confidence: 99%

Recent development of boronic acid-based fluorescent sensors

et al. 2018

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As Lewis acids, boronic acids can bind with 1,2-or 1,3-diols in aqueous solution reversibly and covalently to form five or six cyclic esters, thus resulting in significant fluorescence changes. Based on this phenomenon, boronic acid compounds have been well developed as sensors to recognize carbohydrates or other substances. Several reviews in this area have been reported before, however, novel boronic acid-based fluorescent sensors have emerged in large numbers in recent years. This paper reviews new boronbased sensors from the last five years that can detect carbohydrates such as glucose, ribose and sialyl Lewis A/X, and other substances including catecholamines, reactive oxygen species, and ionic compounds. And emerging electrochemically related fluorescent sensors and functionalized boronic acid as new materials including nanoparticles, smart polymer gels, and quantum dots were also involved.By summarizing and discussing these newly developed sensors, we expect new inspiration in the design of boronic acid-based fluorescent sensors.

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confidence: 99%

Recent development of boronic acid-based fluorescent sensors

et al. 2018

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“…Electrochemical impedance spectroscopy was collected on a CHI 660E (CH Instruments, Shanghai, People's Republic of China) electrochemical workstation at the potential of 0. 25 …”

Section: Procedures For Rhuepo Detectionmentioning

confidence: 99%

“…23,24 Based on the interaction, there has been particular attention in employing boronic acids as recognition units for the immobilization and separation of glycoproteins. 21,[25][26][27] For example, Li et al 25 developed a universal and facile approach for imprinting and detection of five distinct glycoproteins using boronic acid-based ultraviolet (UV)-initiated polymerization and a photolithographic fabrication route. Xu et al 27 prepared the boronic acid-functionalized Au-coated Si wafer as the matrix-assisted laser desorption/ionization for enrichment of glycopeptides.…”

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confidence: 99%

Amplified voltammetric detection of glycoproteins using 4-mercaptophenylboronic acid/biotin-modified multifunctional gold nanoparticles as labels

Liu¹,

Xing²,

Zhang³

et al. 2014

IJN

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Ultrasensitive detection of protein biomarkers is essential for early diagnosis and therapy of many diseases. Glycoproteins, differing from other types of proteins, contain carbohydrate moieties in the oligosaccharide chains. Boronic acid can form boronate ester covalent bonds with diol-containing species. Herein, we present a sensitive and cost-effective electrochemical method for glycoprotein detection using 4-mercaptophenylboronic acid (MBA)/biotin-modified gold nanoparticles (AuNPs) (MBA-biotin-AuNPs) as labels. To demonstrate the feasibility and sensitivity of this method, recombinant human erythropoietin (rHuEPO) was tested as a model analyte. Specifically, rHuEPO was captured by the anti-rHuEPO aptamer-covered electrode and then derivatized with MBA-biotin-AuNPs through the boronic acid–carbohydrate interaction. The MBA-biotin-AuNPs facilitated the attachment of streptavidin-conjugated alkaline phosphatase for the production of electroactive p-aminophenol from p-aminophenyl phosphate substrate. A detection limit of 8 fmol L −1 for rHuEPO detection was achieved. Other glycosylated and non-glycosylated proteins, such as horseradish peroxidase, prostate specific antigen, metallothionein, streptavidin, and thrombin showed no interference in the detection assay.

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“…HRP extraction amount by Fe 3 O 4 @MIPs MNPs is greater than that of Fe 3 O 4 @NIPs MNPs. HRP extracted was detected with chemiluminescence method according to a literature [8]. …”

Section: Effect Of the Polymerization Timementioning

confidence: 99%