Photoluminescent carbon quantum dot/poly-l-Lysine core-shell nanoparticles: A novel candidate for gene delivery

Hasanzadeh, Akbar; Jahromi, Mirza Ali Mofazzal; Abdoli, Amir; Mohammad‐Beigi, Hossein; Fatahi, Yousef; Nourizadeh, Helena; Zare, Hossein; Kiani, Jafar; Radmanesh, Fatemeh; Rabiee, Navid; Jahani, Mehdi; Mombeiny, Reza; Karimi, Mahdi

doi:10.1016/j.jddst.2020.102118

Cited by 30 publications

(26 citation statements)

References 40 publications

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“…Presence of different functional groups on the surface with high density, special architecture and two-dimensional graphene network lead to endow excellent tendency of pseudohomogeneous carrier to bind with DNA. Besides, the DNA fragmentation does not occur due to no extra bonds of DNA are detected in gel electrophoresis proving that the proposed biocompatible nanocarrier meets the standards required to enter a clinical trials 5 , 6 , 39 , 42 , 43 .…”

Section: Resultsmentioning

confidence: 84%

A pseudohomogeneous nanocarrier based on carbon quantum dots decorated with arginine as an efficient gene delivery vehicle

Rezaei

Hashemi

2021

Sci Rep

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A pseudohomogeneous carrier as an emerging term refers to subnanometric carbon-based vehicle with a high ability to interact with genetic materials to form stable carboplex and successfully transfer them into the cell which will result in inhibiting or expressing of therapeutic genes. Chitosan is a non-toxic polyaminosaccharide used as a precursor in the presence of citric acid to produce carbon quantum dots (CQDs), which decorated with arginine as a surface passivation agent with high amine density in hydrothermal methodology. The Arginine-CQDs are comprehensively characterized by Fourier-transform infrared spectroscopy (FT-IR), Ultraviolet–visible spectroscopy (UV–vis), Atomic force microscopy (AFM), field emission scanning electron microscope (FE-SEM), Energy-dispersive X-ray (EDX) mapping, fluorescence, High-resolution transmission electron microscopy (HR-TEM), zeta potential and X-ray powder diffraction (XRD). In this regard, for the first time, carboplex are formed by electrostatic conjugating of Arginine-CQDs with DNA to protect it from enzymatic degradation. Moreover, the carboplex, like the chitosan precursor, has not shown toxicity against AGS cell line. Interestingly, the Arginine-CQDs have exhibited an excellent ability to overcome cell barriers to deliver into cells compared to chitosan at the same weight ratio. The Arginine-CQDs/pEGFP (W/W) nanocomplex, not only lead to transfection with a relatively higher efficiency than PEI polymer, which is the “golden standard”, but carboplex also demonstrates no significant toxicity. Indeed, the EGFP expression level has reached to 2.4 ± 0.2 via Arginine-CQDs carboplex at W/W 50 weight ratio. To the best of our knowledge, this is the first report includes chitosan-based CQDs functionalized by arginine which is applied to serve as a pseudohomogeneous vehicle for gene transfection.

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Section: Resultsmentioning

confidence: 84%

A pseudohomogeneous nanocarrier based on carbon quantum dots decorated with arginine as an efficient gene delivery vehicle

Rezaei

Hashemi

2021

Sci Rep

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“…Usually, a tumor-suppressor gene is incorporated into the tumor cells inhibiting growth in gene therapy or gene delivery. Nevertheless, nanomaterials like CDs have proven efficient application as nanocarriers/gene carriers in gene delivery or gene therapy, owing to their excellent biocompatibility and non-toxicity [303][304][305][306]. Nowadays, the highly transfection efficient CDs are being extensively employed for delivering the nucleic acids such as small interfering RNA (siRNA), noncoding RNAs, and DNA, since the CDs are capable enough to deliver genes via endocytosis, macro-pinocytosis, as well as phagocytosis [43,44,307].…”

Section: Role Of Cds In Gene Delivery or Gene Therapymentioning

confidence: 99%

Carbon Dots: Classification, Properties, Synthesis, Characterization, and Applications in Health Care—An Updated Review (2018–2021)

2021

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Carbon dots (CDs) are usually smaller than 10 nm in size, and are meticulously formulated and recently introduced nanomaterials, among the other types of carbon-based nanomaterials. They have gained significant attention and an incredible interest in the field of nanotechnology and biomedical science, which is merely due to their considerable and exclusive attributes; including their enhanced electron transferability, photobleaching and photo-blinking effects, high photoluminescent quantum yield, fluorescence property, resistance to photo-decomposition, increased electrocatalytic activity, good aqueous solubility, excellent biocompatibility, long-term chemical stability, cost-effectiveness, negligible toxicity, and acquaintance of large effective surface area-to-volume ratio. CDs can be readily functionalized owing to the abundant functional groups on their surfaces, and they also exhibit remarkable sensing features such as specific, selective, and multiplex detectability. In addition, the physico-chemical characteristics of CDs can be easily tunable based on their intended usage or application. In this comprehensive review article, we mainly discuss the classification of CDs, their ideal properties, their general synthesis approaches, and primary characterization techniques. More importantly, we update the readers about the recent trends of CDs in health care applications (viz., their substantial and prominent role in the area of electrochemical and optical biosensing, bioimaging, drug/gene delivery, as well as in photodynamic/photothermal therapy).

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“…Hence, poly-L-lysine-functionalized GQDs have great potential in biomedicine as biolabeling agents. 4 Sreeprasad T. S. et al functionalized GQDs with PLL, which can prociently electrostatically assemble on the walls of Grampositive Bacillus subtilis endospore. The transportation of water through its membrane offers high-energy sporal hydraulics (driving force: 299.75 Torrs (21.7% water @ GQD junctions)).…”

Section: Biomedical Sensorsmentioning

confidence: 99%

“…The wide-ranging properties of CQDs and GQDs allow their exploration in almost all the elds including biological sciences, 3,4 electronics, 5 energy devices, 6 sensors, 7 agricultural, 8 cosmetics, 9 pharmaceuticals, 10 and medical devices. 11 Both the QDs possess characteristic properties such as low toxicity, great solubility in a variety of solvents, good optoelectronic behavior, strong inertness toward chemicals, high surface area, and surface edges for functionalization.…”

Section: Introductionmentioning

confidence: 99%

Do amino acid functionalization stratagems on carbonaceous quantum dots imply multiple applications? A comprehensive review

et al. 2021

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Photoluminescent carbon quantum dot/poly-l-Lysine core-shell nanoparticles: A novel candidate for gene delivery

Cited by 30 publications

References 40 publications

A pseudohomogeneous nanocarrier based on carbon quantum dots decorated with arginine as an efficient gene delivery vehicle

A pseudohomogeneous nanocarrier based on carbon quantum dots decorated with arginine as an efficient gene delivery vehicle

Carbon Dots: Classification, Properties, Synthesis, Characterization, and Applications in Health Care—An Updated Review (2018–2021)

Do amino acid functionalization stratagems on carbonaceous quantum dots imply multiple applications? A comprehensive review

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