Photolysis and Photo‐oxidation of Amino Acids and Peptides—vii. Photodegradation and Radiolysis of Some S‐alkylcysteines.

Hydrogen Exchange at Carbon-Hydrogen Sites during Acid or Alkaline Treatment of Proteins

“…Butanol-acetic acid-water (4:1:1) was used in the first dimension, and phenol-water (4:1) in the second dimension (Berry et al, 1951). For a clearer separation of tyrosine and the three basic amino acids a system devised by Eager et al (1964) was also used. This involved paper electrophoresis at pH 2.3 (1 m acetic acid) in one direction, followed by chromatography with 2-butanol-2-methoxyethanol-formic acid-water (8:2:1:3) at right angles.…”