Photometric aptasensor using biofunctionalized photonic crystal slabs

Jahns, Sabrina; Nazirizadeh, Yousef; Meyer, Björn-Ole; Gerken, Martina; Gutekunst, Sören B.; Selhuber‐Unkel, Christine

doi:10.1109/icsens.2013.6688384

Cited by 3 publications

(3 citation statements)

References 10 publications

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“…Previously, optical read-out of the resonance spectral position was evaluated with a sensitive spectrometer for other microfluidic devices [9]. We developed an imaging read-out system, which converts the resulting shift of resonances in the transmission spectrum into an intensity change in the specific colour channel of a simple CMOS camera aligned to the resonance wavelength [11]. With such a system we are able to detect multiple biomarkers in parallel.…”

Section: Introductionmentioning

confidence: 99%

Human blood microfluidic test chip for imaging, label-free biosensor

et al. 2015

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We designed and evaluated a microfluidic test chip for human blood filtration and imaging label-free detection of multiple biomarkers. The microfluidic chip has a total size of 75 mm x 25 mm x 2 mm. It is realized as an assembly of a plastic chip and a functionalized photonic crystal slab on a glass substrate. The plastic chip contains capillary channels, a filter membrane and a cavity open on one side. The photonic crystal chip is bonded with an adhesive foil to the open cavity. Human blood filtration is demonstrated. We determined that fluorescently labelled particles of diameter 3µm or larger are filtered out by the system. Refractometric measurements are performed with the test chip in combination with a compact imaging read-out system to investigate the system response to refractive index changes. Flow dynamics in the sensor cavity are imaged for replacing water by isopropanol. Finally, the binding of 500 nM biotin dissolved in phosphate buffer saline to a photonic crystal surface locally functionalized with streptavidin is demonstrated.

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Section: Introductionmentioning

confidence: 99%

Human blood microfluidic test chip for imaging, label-free biosensor

et al. 2015

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“…For the analyte exchange two butterfly cannulas are pierced through the o-ring and all is fixed with two screwed substrate holders. Figure 3a depicts a photograph of the employed measurement system [12]. The imaging read-out system has an overall size of 13 cm x 3.5 cm x 4.9 cm and the whole lens system, two crossed polarizing filters and a light emitting diode (LED) are placed inside the metallic torso.…”

Section: Protein Detection With Fluid Cellmentioning

confidence: 99%

“…Previously, for other microfluidic devices optical read-out of the resonance spectral position was evaluated with a sensitive spectrometer [9]. With our imaging read-out system the resulting shift of resonances in the transmission spectrum is converted into an intensity change in the specific color channel of a simple CMOS camera aligned to the resonance wavelength [12]. Especially for point-of-care applications a test chip, which is able to detect multiple biomarkers in whole blood, combined with a compact and portable read-out system is of high interest.…”

Section: Introductionmentioning

confidence: 99%

Imaging label-free biosensor with microfluidic system

et al. 2015

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We present a microfluidic system suitable for parallel label-free detection of several biomarkers utilizing a compact imaging measurement system. The microfluidic system contains a filter unit to separate the plasma from human blood and a functionalized, photonic crystal slab sensor chip. The nanostructure of the photonic crystal slab sensor chip is fabricated by nanoimprint lithography of a period grating surface into a photoresist and subsequent deposition of a TiO 2 layer. Photonic crystal slabs are slab waveguides supporting quasi-guided modes coupling to far-field radiation, which are sensitive to refractive index changes due to biomarker binding on the functionalized surface. In our imaging read-out system the resulting resonance shift of the quasi-guided mode in the transmission spectrum is converted into an intensity change detectable with a simple camera. By continuously taking photographs of the sensor surface local intensity changes are observed revealing the binding kinetics of the biomarker to its specific target. Data from two distinct measurement fields are used for evaluation. For testing the sensor chip, 1 µM biotin as well as 1 µM recombinant human CD40 ligand wereimmobilized in spotsvia amin coupling to the sensor surface. Each binding experiment was performed with 250 nM streptavidin and 90 nM CD40 ligand antibody dissolved in phosphate buffered saline. In the next test series, a functionalized sensor chip was bonded onto a 15 mm x 15 mm opening of the 75 mm x 25 mm x 2 mm microfluidic system. We demonstrate the functionality of the microfluidic system for filtering human blood such that only blood plasma was transported to the sensor chip. The results of first binding experiments in buffer with this test chip will be presented.

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Imaging label-free biosensor for multiplexed protein detection

Jahns

Gerken

2016

2016 18th International Conference on Transparent Optical Networks (ICTON)

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Photometric aptasensor using biofunctionalized photonic crystal slabs

Cited by 3 publications

References 10 publications

Human blood microfluidic test chip for imaging, label-free biosensor

Human blood microfluidic test chip for imaging, label-free biosensor

Imaging label-free biosensor with microfluidic system

Imaging label-free biosensor for multiplexed protein detection

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