Photometric Assay of Dopamine-β-Hydroxylase Activity in Human Blood

Nagatsu, Toshiharu; Udenfriend, Sidney

doi:10.1093/clinchem/18.9.980

Cited by 493 publications

(65 citation statements)

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“…Dopamine-4-hydroxylase activity DBH activity with tyramine as substrate (Nagatsu & Udenfriend, 1972) was measured spectrophotometrically in the adrenal glands of female rats after 3 weeks of treatment. (open column) and female (stippled column) and chronic EO-treated male (hatched column) and female (solid column) rats.…”

Section: Experiments On Ethinyl Oestradiol-treated and Untreated Contmentioning

confidence: 99%

The role of the sympathetic nervous system in oestrogen‐induced hypertension in rats

Bhatt

Gulati

1986

British J Pharmacology

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Albino rats of either sex received chronic ethinyl oestradiol (EO) treatment (1.5 mg kg−1 daily, i.m.) for 3 weeks. Untreated control rats received arachis oil vehicle alone. Chronic EO treatment resulted in elevation of blood pressure in both sexes. Female rats exhibited significantly greater elevation in blood pressure than males. In chronic EO‐treated rats pressor responses to low doses (0.5 μg kg−1) of noradrenaline were significantly increased, while those to angiotensin II, acetylcholine and isoprenaline were unaltered. Chronic EO treatment also sensitized the vascular bed of the rats' hindquarters to noradrenaline. EO‐induced hypertension was associated with significant increase in dopamine‐β‐hydroxylase activity of adrenal glands. Complete bilateral adrenalectomy or chemical sympathectomy prevented the development of EO‐induced hypertension. It is suggested that chronic treatment of rats with EO induces and maintains hypertension. The peripheral sympathetic system plays an important role in this phenomenon.

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Section: Experiments On Ethinyl Oestradiol-treated and Untreated Contmentioning

confidence: 99%

The role of the sympathetic nervous system in oestrogen‐induced hypertension in rats

Bhatt

Gulati

1986

British J Pharmacology

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“…The radioenzymatic assay for CAs is based on the measurement of the 3-O-methyl-3H derivatives of CAs resulting from incubation of test solutions containing CAs with S-adenosyl-L-methionine [methyl-3H] (specific activity 6 to 10 Ci/mmol, Amersham) and partially purified rat liver catechol-O-methyltransferase. The activity of DBH in plasma was determined by the method of Nagatsu & Udenfriend (1972) with the modifications suggested by Kato, Kuzuya & Nagatsu (1974). Enzyme activity was expressed as nanomoles of octopamine formed per ml of plasma per minute.…”

mentioning

confidence: 99%

Adrenal Origin of Plasma Catecholamines After Decapitation: A Study in Normal and Diabetic Rats

Berkowitz

Head

1978

British J Pharmacology

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The concentrations of catecholamines and the activities of dopamines‐hydroxylase were measured in blood obtained from decapitated diabetic and aged‐matched control rats. The activity of dopamines‐hydroxylase in blood from diabetic rats was much greater (5 fold) than that seen for control rats. For both diabetic and control rats, decapitation was accompanied by an increase in levels of adrenaline and noradrenaline with no change in the activity of plasma dopamines‐hydroxylase. The results are consistent with a predominantly adrenal origin of catecholamines and extra‐adrenal origin of dopamine‐β‐hydroxylase. The high activity of dopamines‐hydroxylase in diabetes indicates either an increased activity of the sympathetic nervous system or changes in dopamines‐hydroxylase turnover.

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“…T h e enzyme gains entrance to the peripheral circulation, where it is assayable by rapid enzymatic techniques. 9 T h e current approach to the radio-and chemotherapy of neoplastic disease is predicated on the concept of rapid, early diagnosis, and institution of appropriate therapy. T h e case herein reported exemplifies such an approach: the application of a relatively new tool, the assay of plasma-DBH activity, facilitated the diagnosis of an atypical presentation of neuroblastoma.…”

Section: Discussionmentioning

confidence: 99%