Photometric determination of ammonium nitrogen with the nessler reagent in drinking water after its chlorination

Demutskaya, L. N.; Kalinichenko, I. E.

doi:10.3103/s1063455x10020049

Cited by 49 publications

(19 citation statements)

References 6 publications

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“…Broth was collected, centrifuged and the amount of ammonia in the supernatant was estimated by means of Nesslerization reaction 1ml Nessler's reagent was added to 1ml of supernatant and volume of this mixture was made up to 10ml by addition of ammonia-free distilled water. Development of brown to yellow color was a positive test for ammonia production and optical density was measured by spectrophotometer at 450 nm (Demutskaya & Kalinichenko 2010). The concentration of Ammonia was estimated based on a standard curve of ammonium sulfate ranging from 0.1 to 1 mmol ml (1 .…”

Section: Ammonia Productionmentioning

confidence: 99%

Plant growth promoting potentials ofPseudomonasspp. strain OG isolated from marine water

Goswami

Vaghela

Parmar

et al. 2013

Journal of Plant Interactions

103

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Bacterium Pseudomonas spp. olive green (OG) was isolated from marine water, yet, it was characterized as plant growth promoting bacterium (PGPB). Multiple plant growth promoting traits of OG isolate were determined in vitro. It was tested positive for Indole-3-acetic acid (IAA) production with 29 mg ml (1 of IAA yield, phosphate solubilization with 34 mg ml (1 solubilization of Tri-calcium-phosphate and it showed maximum of 32 mg ml (1 of ammonia production. OG isolate was affirming siderophore production, hydrocyanic acid (HCN) production and catalase production. 16S rRNA gene sequence comparison was used to identify the isolate which showed its closest neighbor to be Pseudomonas fluoroscens strain BCPBMS-1. Efficacy of this PGPB was tested on the seedling growth of two test plants chickpea and green gram. Both the test plants treated with OG-based talc bioformulation showed significant growth promotion. Chickpea showed enhanced overall fresh biomass by 24%, overall dry biomass by 27% was observed after 15 days of seeded in pots. Green gram showed enhanced overall dry biomass by 28% was observed after 10 days of seeded in pots.

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Section: Ammonia Productionmentioning

confidence: 99%

Plant growth promoting potentials ofPseudomonasspp. strain OG isolated from marine water

Goswami

Vaghela

Parmar

et al. 2013

Journal of Plant Interactions

103

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“…Traditional methods for measuring ammonia content were based on Nessler's reagent colorimetry [3], indophenol [4], Ophthaldialdehyde (OPA) and N-acetyl-cysteine (NAC) reagent [5], and titration [6]. Electrochemical methods have attracted great interests in recent years because of its high sensitivity, fast analysis, low detection limit, few reagent requirements, and little cost [7].…”

Section: Introductionmentioning

confidence: 99%

A novel all-solid-state ammonium electrode with polyaniline and copolymer of aniline/2,5-dimethoxyaniline as transducers

Huang

Yin

et al. 2015

Journal of Electroanalytical Chemistry

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“…The concentration of ammonium nitrogen in CM was measured by the Nessler method using alkaline iodide (Demutskaya & Kalinichenko, 2010) considering the effects of temperature and pH (Hampson, 1977) and salinity (Bower & Bidwell, 1978).…”

Section: The Analytical Methodsmentioning

confidence: 99%

A novel growth-promoting protein in the conditioned media from the rotifer Brachionus plicatilis at an early exponential growth phase

et al. 2011

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We confirmed the existence of growthpromoting substances in the conditioned media (CM) from the rotifer Brachionus plicatilis at an early exponential growth phase and isolated a novel protein with a growth-promoting activity from the crude extract (CE) of rotifer cells. CM was prepared from the culture media where rotifers had been cultured at an early exponential growth phase and filtered through a 0.22-lm filter membrane. The growth-promoting activity was determined using rotifers in CM for 5 days. As a result, the increase of rotifers added with CM was significantly higher than that of the control in artificial seawater (P \ 0.001). Moreover, the growthpromoting activity of CM was dose-dependent and inactivated by heat treatment at 80°C for 60 min. Meanwhile, CM filtered through a \10 kDa ultrafiltration membrane showed a low activity, whereas proteinase K treatment resulted in a complete inactivation. These results suggest that the rotifer secrets growth-promoting proteins into CM. CE also contained a protein with the activity and properties similar to those found in CM. Then, CE was subjected to purification of a growth-promoting protein for convenience using various types of chromatography after fractionation with 30-80% saturated ammonium sulfate. Subsequently, a protein with an approximate molecular weight of 25000 was isolated, and its N-terminal amino acid sequence was determined to be PAVVDFTAVWFGPLQMIKP. An orthologue was found in the EST database of B. plicatilis, the full sequence of which showed about 50% identity to the corresponding regions of thioredoxins from other organisms.

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Photometric determination of ammonium nitrogen with the nessler reagent in drinking water after its chlorination

Cited by 49 publications

References 6 publications

Plant growth promoting potentials ofPseudomonasspp. strain OG isolated from marine water

Plant growth promoting potentials ofPseudomonasspp. strain OG isolated from marine water

A novel all-solid-state ammonium electrode with polyaniline and copolymer of aniline/2,5-dimethoxyaniline as transducers

A novel growth-promoting protein in the conditioned media from the rotifer Brachionus plicatilis at an early exponential growth phase

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