Photometric Evaluation of the Solid‐Phase Antiglobulin Test Using Length Measurement of the Absorption Curve

Abstract

The new method allows the complete automation of the solid-phase antiglobulin test, enabling the analysis and evaluation of the solid-phase microtiter plate assay to be more discriminating and safe.

A novel microplate agglutination method for blood grouping and reverse typing without the need for centrifugation

“…Subsequently, 9 × 25 μL from the suspension of RBCs in diluent were transferred into the appropriate wells of the second native V‐bottom microplate. Following an incubation period at room temperature of 40 minutes the results were read by photometer at 405 nm as previously described 7 …”

“…This report describes a novel automated photometric analysis and evaluation assay for the hemagglutination reaction, independent of the sedimentation position of RBCs in the wells of a microplate and capable of detecting a weakly positive reaction 7 . Further, it introduces a microplate agglutination method (MAM) based on conventional microplates without the necessity of immobilization of either antigen or antibody and centrifugation.…”