Photometric Method for the Quantification of Chlorophylls and Their Derivatives in Complex Mixtures: Fitting with Gauss-Peak Spectra

Küpper, Hendrik; Spiller, Martin; Küpper, Frithjof C.

doi:10.1006/abio.2000.4794

Cited by 64 publications

(61 citation statements)

References 8 publications

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“…Samples were lyophilized and ground with sand and a few grains of Bis-Tris (Sigma-Aldrich, St Louis, MO, USA) and extraction of pigments was performed in 1 ml 100% acetone at 4°C overnight. The pigment extraction and determination were performed using established protocols by Küpper et al (2007bKüpper et al ( , 2000b as described in Thomas et al (2013). Values are given as means of three different experiments.…”

Section: Determination Of Pigment Contentmentioning

confidence: 99%

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Effects of nanomolar copper on water plants—Comparison of biochemical and biophysical mechanisms of deficiency and sublethal toxicity under environmentally relevant conditions

et al. 2013

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Section: Determination Of Pigment Contentmentioning

confidence: 99%

“…Pigments of the harvested material were extracted and determined following established protocols by Küpper et al (2007bKüpper et al ( , 2000b.…”

Section: Determination Of Pigment Contentmentioning

confidence: 99%

Effects of nanomolar copper on water plants—Comparison of biochemical and biophysical mechanisms of deficiency and sublethal toxicity under environmentally relevant conditions

et al. 2013

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“…Spectra of pigment extracts were measured with the UV/visible spectrophotometer Lambda 16 (Perkin-Elmer) at a spectral bandwidth of 1 nm with 0.2-nm sampling interval from 350 to 750 nm. Chls were quantified according to the "Gauss-peak-spectra" (GPS) method developed by Kü pper et al (2000bKü pper et al ( , 2007b. Normalized spectra (350-750 nm) of pigment standards were mathematically described by a series of Gaussian peaks.…”

Section: Quantification Of Chl In Plant Extractsmentioning

confidence: 99%

Complexation and Toxicity of Copper in Higher Plants. I. Characterization of Copper Accumulation, Speciation, and Toxicity inCrassula helmsiias a New Copper Accumulator

et al. 2009

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The amphibious water plant Crassula helmsii is an invasive copper (Cu)-tolerant neophyte in Europe. It now turned out to accumulate Cu up to more than 9,000 ppm in its shoots at 10 mM (=0.6 ppm) Cu 2+ in the nutrient solution, indicating that it is a Cu hyperaccumulator. We investigated uptake, binding environment, and toxicity of Cu in this plant under emerged and submerged conditions. Extended x-ray absorption fine structure measurements on frozen-hydrated samples revealed that Cu was bound almost exclusively by oxygen ligands, likely organic acids, and not any sulfur ligands. Despite significant differences in photosynthesis biochemistry and biophysics between emerged and submerged plants, no differences in Cu ligands were found. While measurements of tissue pH confirmed the diurnal acid cycle typical for Crassulacean acid metabolism, D 13 C measurements showed values typical for regular C3 photosynthesis. Cu-induced inhibition of photosynthesis mainly affected the photosystem II (PSII) reaction center, but with some unusual features. Most obviously, the degree of light saturation of electron transport increased during Cu stress, while maximal dark-adapted PSII quantum yield did not change and light-adapted quantum yield of PSII photochemistry decreased particularly in the first 50 s after onset of actinic irradiance. This combination of changes, which were strongest in submerged cultures, shows a decreasing number of functional reaction centers relative to the antenna in a system with high antenna connectivity. Nonphotochemical quenching, in contrast, was modified by Cu mainly in emerged cultures. Pigment concentrations in stressed plants strongly decreased, but no changes in their ratios occurred, indicating that cells either survived intact or died and bleached quickly.

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“…Chl was assayed by filtration on 5-mm polycarbonate filters, extraction with 100% acetone, recording the absorbance spectra from 550 to 750 nm recorded, and analyzing the spectra using the Gauss-Peak-Spectra method according to Kü pper et al (2000b).…”

Section: Chl Concentrationmentioning

confidence: 99%

Traffic Lights in Trichodesmium. Regulation of Photosynthesis for Nitrogen Fixation Studied by Chlorophyll Fluorescence Kinetic Microscopy

Küpper

Ferimazova²,

Šetlík³

et al. 2004

Plant Physiology

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We investigated interactions between photosynthesis and nitrogen fixation in the non-heterocystous marine cyanobacterium Trichodesmium IMS101 at the single-cell level by two-dimensional (imaging) microscopic measurements of chlorophyll fluorescence kinetics. Nitrogen fixation was closely associated with the appearance of cells with high basic fluorescence yield (F 0 ), termed bright cells. In cultures aerated with normal air, both nitrogen fixation and bright cells appeared in the middle of the light phase. In cultures aerated with 5% oxygen, both processes occurred at a low level throughout most of the day. Under 50% oxygen, nitrogen fixation commenced at the beginning of the light phase but declined soon afterwards. Rapid reversible switches between fluorescence levels were observed, which indicated that the elevated F 0 of the bright cells originates from reversible uncoupling of the photosystem II (PSII) antenna from the PSII reaction center. Two physiologically distinct types of bright cells were observed. Type I had about double F 0 compared to the normal F 0 in the dark phase and a PSII activity, measured as variable fluorescence (F v 5 F m 2 F 0 ), similar to normal non-diazotrophic cells. Correlation of type I cells with nitrogen fixation, oxygen concentration, and light suggests that this physiological state is connected to an up-regulation of the Mehler reaction, resulting in oxygen consumption despite functional PSII. Type II cells had more than three times the normal F 0 and hardly any PSII activity measurable by variable fluorescence. They did not occur under low-oxygen concentrations, but appeared under high-oxygen levels outside the diazotrophic period, suggesting that this state represents a reaction to oxidative stress not necessarily connected to nitrogen fixation. In addition to the two high-fluorescence states, cells were observed to reversibly enter a low-fluorescence state. This occurred mainly after a cell went through its bright phase and may represent a fluorescence-quenching recovery phase.Biological fixation of atmospheric nitrogen is performed by certain cyanobacteria when bioavailable forms of nitrogen (nitrate and ammonia) are limited. Nitrogen fixation is catalyzed by an essentially anaerobic enzyme, nitrogenase, which is irreversibly inhibited in vitro when exposed to molecular oxygen (for review, see Postgate, 1998). Diazotrophic cyanobacteria are the only nitrogen-fixing organisms that produce molecular oxygen as a by-product of photosynthesis and must prevent nitrogenase from being damaged by oxygenic photosynthesis (for review, see Gallon, 1992Gallon, , 2001Bergman et al., 1997;Berman-Frank et al., 2003). Most diazotrophic cyanobacteria achieve this by separating photosynthesis and nitrogen fixation either spatially, by differentiating highly specialized cells called heterocysts, or temporally, by fixing nitrogen at night (usually found in unicellular diazotrophic cyanobacteria). In contrast, filamentous non-heterocystous marine cyanobacteria of the genus Trichodesmium execut...

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Photometric Method for the Quantification of Chlorophylls and Their Derivatives in Complex Mixtures: Fitting with Gauss-Peak Spectra

Cited by 64 publications

References 8 publications

Effects of nanomolar copper on water plants—Comparison of biochemical and biophysical mechanisms of deficiency and sublethal toxicity under environmentally relevant conditions

Effects of nanomolar copper on water plants—Comparison of biochemical and biophysical mechanisms of deficiency and sublethal toxicity under environmentally relevant conditions

Complexation and Toxicity of Copper in Higher Plants. I. Characterization of Copper Accumulation, Speciation, and Toxicity inCrassula helmsiias a New Copper Accumulator

Traffic Lights in Trichodesmium. Regulation of Photosynthesis for Nitrogen Fixation Studied by Chlorophyll Fluorescence Kinetic Microscopy

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