Photon avalanche in lanthanide doped nanoparticles for biomedical applications: super-resolution imaging

Bednarkiewicz, A.; Chan, Emory M.; Kotulska, Agata; Marciniak, Ł.; Prorok, Katarzyna

doi:10.1039/c9nh00089e

Cited by 66 publications

(92 citation statements)

References 39 publications

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“…8b, c), which allows the emission saturation mode to be used for super-resolution imaging 31 . Note that instead of the upconversion stimulated emission depletion (STED) superresolution microscopy configuration [12][13][14]32 that only works well for depleting the emissions from UCNPs highly doped with Tm 3+ ions, our current method is a lot simpler and broadly compatible with different lanthanide emitters, doping concentrations, and emission bands 31 . The design of a single beam donut illumination avoids the sophisticated system alignment and temporal synchronization of both Gaussian excitation and donut depletion beams required by STED.…”

Section: Resultsmentioning

confidence: 99%

“…Due to a wealth of electronic transitions within the 4f electron shells of lanthanide ions, nanostructures doped with lanthanide ions can form a unique class of functional optical devices 6,7 . Among them, spherical upconversion nanoparticles (UCNPs) have been created with many unique optical properties, including tuneable colors 8 , multiplexed lifetimes 9,10 , long-distance energy migration 11 , amplified stimulated emissions [12][13][14][15][16] and their responses to external fields of temperature 17 and mechanical force 18 , which enables many novel applications, including fullcolor displays 8 , solar energy harvesting 19 , security inks 9 , biomolecular sensing 20 , force sensing 21 , nanothermometry 22,23 , fluorescence microscopy 12 , optical multiplexing 9,24 , deep-tissue optogenetics 25 , multimodal bio-imaging 26,27 , and light-triggered drug delivery 28,29 .…”

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confidence: 99%

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Nanorods with multidimensional optical information beyond the diffraction limit

et al. 2020

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Precise design and fabrication of heterogeneous nanostructures will enable nanoscale devices to integrate multiple desirable functionalities. But due to the diffraction limit (~200 nm), the optical uniformity and diversity within the heterogeneous functional nanostructures are hardly controlled and characterized. Here, we report a set of heterogeneous nanorods; each optically active section has its unique nonlinear response to donut-shaped illumination, so that one can discern each section with super-resolution. To achieve this, we first realize an approach of highly controlled epitaxial growth and produce a range of heterogeneous structures. Each section along the nanorod structure displays tunable upconversion emissions, in four optical dimensions, including color, lifetime, excitation wavelength, and power dependency. Moreover, we demonstrate a 210 nm single nanorod as an extremely small polychromatic light source for the on-demand generation of RGB photonic emissions. This work benchmarks our ability toward the full control of sub-diffraction-limit optical diversities of single heterogeneous nanoparticles.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Nanorods with multidimensional optical information beyond the diffraction limit

et al. 2020

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“…Except for the canonical super-resolution techniques, multiphoton probes with high order of non-linearity favors higher spatial resolution (Yu et al, 2013 ; Bednarkiewicz et al, 2019 ). In multiphoton imaging, the imaging resolution approximately equals to λ/(2 × √ N ), where λ and N denote the excitation wavelength and the order of non-linearity, respectively.…”

Section: Perspectives Of Ucnps-based Super-resolution Imagingmentioning

confidence: 99%

Lanthanide-Doped Upconversion Nanoparticles for Super-Resolution Microscopy

2021

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Super-resolution microscopy offers a non-invasive and real-time tool for probing the subcellular structures and activities on nanometer precision. Exploring adequate luminescent probes is a great concern for acquiring higher-resolution image. Benefiting from the atomic-like transitions among real energy levels, lanthanide-doped upconversion nanoparticles are featured by unique optical properties including excellent photostability, large anti-Stokes shifts, multicolor narrowband emissions, tunable emission lifetimes, etc. The past few years have witnessed the development of upconversion nanoparticles as probes for super-resolution imaging studies. To date, the optimal resolution reached 28 nm (λ/36) for single nanoparticles and 82 nm (λ/12) for cytoskeleton structures with upconversion nanoparticles. Compared with conventional probes such as organic dyes and quantum dots, upconversion nanoparticle-related super-resolution microscopy is still in the preliminary stage, and both opportunities and challenges exist. In this perspective article, we summarized the recent advances of upconversion nanoparticles for super-resolution microscopy and projected the future directions of this emerging field. This perspective article should be enlightening for designing efficient upconversion nanoprobes for super-resolution imaging and promote the development of upconversion nanoprobes for cell biology applications.

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“…Due to a wealth of electronic transitions within the 4f electron shells of lanthanide ions, nanostructures doped with lanthanide ions can form a unique class of functional optical devices 6,7 . Among them, spherical upconversion nanoparticles (UCNPs) have been created with many unique optical properties, including tuneable colors 8 , multiplexed lifetimes 9,10 , long-distance energy migration 11 , ampli ed stimulated emissions [12][13][14][15][16] and their responses to external elds of temperature 17 and mechanical force 18 , which enables many novel applications, including full-colour displays 8 , solar energy harvesting 19 , security inks 9 , biomolecular sensing 20 , force sensing 21 , nanothermometry 22,23 , uorescence microscopy 12 , optical multiplexing 24 , deep-tissue optogenetics 25 , multimodal bio-imaging 26,27 and lighttriggered drug delivery 28,29 .…”

Section: Introductionmentioning

confidence: 99%

Nanobarcodes with multidimensional optical information beyond diffraction limit

Jin

Wen

Liu

et al. 2020

Preprint

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Abstract Precise design and fabrication of heterogeneous nanostructures will enable nanoscale devices to integrate multiple desirable functionalities. But due to the diffraction limit (~200 nm), the optical uniformity and diversity within the heterogeneous functional nanostructures are hardly controlled and characterized. Here we report a set of nanobarcodes, each optically active section has its unique nonlinear responses to donut illumination patterns, so that one can discern each unit with super resolution. To achieve this, we first realized an approach of highly controlled epitaxial growth and produced a range of one-dimensional heterogeneous structures. Each section along the nanorod structure display tunable upconversion emissions, in four optically orthogonal dimensions, including colour, lifetime, excitation wavelength, and power dependency. Moreover, we demonstrated a 210 nm single nanorod as the smallest polychromatic light source for the on-demand generation of RGB photonic emissions. Remarkably, within a space of 50 nm, only 1/20th of the excitation wavelength, multiple codes can be successfully coded and decoded in 4 optical dimensions. This precision control enables the fabrication of super capacity geometrical barcodes with theoretical coding capacity up to (24-1)4. This work benchmarks our new ability towards the full control of sub-diffraction-limit optical diversities of single heterogeneous nanoparticles.

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Photon avalanche in lanthanide doped nanoparticles for biomedical applications: super-resolution imaging

Abstract: Photon avalanche in lanthanide doped nanoparticles shows exceptional properties, potentially suitable for single photoexcitation beam sub-diffraction imaging.

Cited by 66 publications

References 39 publications

Nanorods with multidimensional optical information beyond the diffraction limit

Nanorods with multidimensional optical information beyond the diffraction limit

Lanthanide-Doped Upconversion Nanoparticles for Super-Resolution Microscopy

Nanobarcodes with multidimensional optical information beyond diffraction limit

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