Waveguide is an essential building block of integrated optics. Using finite-difference time-domain method, waveguide in woodpile structure, which the dielectric constants for background media and dielectric rods are 1.0 and 9.0, is designed, analyzed, and simulated. Since, woodpile structure has periodical property in stacking direction, off-plane adjustment is considered. Our results show that the guide band can be also effectively adjusted by varying dielectric constant