Photonic Nanojet‐Mediated Optogenetics

Abstract

In the original published article, there was a mistake in Figure 2I. Please find the correct Figure 2 below.Figure 2. PS microsphere enhances light-evoked neuron activity via ChR2. A) Schematic configuration for in vitro experiment. Whole-cell patch clamp recording was conducted in ChR2-expressed cells stimulated by blue light in the presence of PS (PS(+)) or not (PS(−)). B) Inward currents induced by a 100 ms light pulse with or without PS in 293T cell with ChR2. C) The curve between input power density and inward currents induced by light with PS or without PS in 293T cells, n = 5, *p < 0.05, **p < 0.01, ***p < 0.001, unpaired two-tailed t-test. D) Inward currents of the target neurons as a function of the input power density. E) Primary hippocampal neurons expressing ChR2-mCherry in bright filed and 488 nm excitation without PS or with PS (scale bar 100 μm). F) Inward currents induced by 20 ms light pulse with or without PS in primary hippocampus neuron with ChR2. n = 10, ***p < 0.001 unpaired two-tailed t-test. G) The curve between input power density and inward currents induced by light with PS or without PS in primary hippocampus neuron. H) Inward currents of the target neurons as a function of the input power density. I) Current clamp recording of hippocampus neuron stimulated with 20 ms pulse light without or with PS. J) Success rate of generation of action potential evoked by light pulse in different power density. n = 3, **p < 0.01, unpaired two-tailed t-test.

Emerging optogenetics technologies in biomedical applications

“…[3][4][5][6][7][8] Optogenetics has several advantages over other tools for targeted regulation of cells, such as chemical methods, pure genetic methods, and hybridization methods. [9][10][11] By employing light as a trigger, optogenetics offers meticulous temporal and spatial control over cellular expression, resulting in prompt responsiveness and high-intensity manipulability. [12][13][14][15] Optogenetics is not only applicable to the rapid regulation of neuronal expression and neurological timing but also to the regulation of cell, tissue, and gene expression.…”

“…Examples of light regulation include the phototropism, light‐dependent growth response of plants, the visual perception of animals, and the photophilic movement of bacteria 3–8 . Optogenetics has several advantages over other tools for targeted regulation of cells, such as chemical methods, pure genetic methods, and hybridization methods 9–11 . By employing light as a trigger, optogenetics offers meticulous temporal and spatial control over cellular expression, resulting in prompt responsiveness and high‐intensity manipulability 12–15 …”