Photopatterned Antibodies for Selective Cell Attachment

Abstract

We present a phototriggerable system that allows for the spatiotemporal controlled attachment of selected cell types to a biomaterial using immobilized antibodies that specifically target individual cell phenotypes. o-Nitrobenzyl caged biotin was used to functionalize chitosan membranes and mediate site-specific coupling of streptavidin and biotinylated antibodies after light activation. The ability of this system to capture and immobilize specific cells on a surface was tested using endothelial-specific biotinylated antibodies and nonspecific ones as controls. Homogeneous patterned monolayers of human umbilical vein endothelial cells were obtained on CD31-functionalized surfaces. This is a simple and generic approach that is applicable to other ligands, materials, and cell types and shows the flexibility of caged ligands to trigger and control the interaction between cells and biomaterials.

Strategies to develop endogenous stem cell-recruiting bioactive materials for tissue repair and regeneration

“…Custodio et al . achieved successful attachment of endothelial cells by immobilizing antibodies to chitosan gels with micropatterning techniques [71]. Chitosan films were chemically modified with a UV photocleavable biotin derivative, which was coupled with streptavidin and biotinylated CD31 antibody.…”

Chitosan to Connect Biology to Electronics: Fabricating the Bio-Device Interface and Communicating Across This Interface

“…For instance, biotin moieties have been covalently attached to chitosan and then proteins have been assembled to the biotinylated chitosan through biospecific (strept)avidin-biotin binding [158,159]. In addition, chitosan's ability to chelate metals (e.g., nickel) enables a nickel-mediated assembly of proteins if the proteins have been engineered with hexa-histidine fusion tags that are commonly used to facilitate protein purification [160].…”

Cell-free vascular grafts: Recent developments and clinical potential

“…These grafts exhibited high patency rates in the high flow environment of ovine carotid arteries and produced fully endothelialized, functional arteries as soon as one month post-implantation 50 . Unlike antibodies which have also been applied to capture EPCs from the host bloodstream 51,52 , VEGF enhances the survival, proliferation and cell spreading of captured progenitors 53–56 , making it a more attractive option for functionalizing TEBVs.…”