Photoperiod Affects <i>in Vitro</i> Flowering in Wild Peanut <i>Arachis paraguariensis</i>

Abstract

Arachis paraguariensis, a wild peanut species, is a potential experimental system for studying the molecular mechanisms of flowering in the genus Arachis. The present study was carried out to investigate the effect of photoperiod on in vitro reproductive behavior of five genotypes of A. paraguariensis. Day-lengths of 12, 16 and 24 h were tested to monitor in vitro flowering using growth chambers kept at 26˚C ± 1˚C and 60% ± 5% relative humidity under an illumination of 40 μmol·m . Flowering percentage of plantlets ranged from 35% to 93%, 20% to 75%, and 5% to 53% for 12, 16 and 24 h day-lengths, respectively. Genotype PI 262842 displayed the highest frequency of flowering under all the day-length treatments but in vitro flower bud initiation was delayed. The highest mean flowering percentage of 65% across all the genotypes for plantlets exposed to 12 h photoperiod is indicative that flowering induction actually occurred. The results presented in this paper provide evidence for photoperiodic flowering response as well as the occurrence of short day-length-enhanced flowering in A. paraguariensis.

Synergistic effect of BAP and GA3 on in vitro flowering of Guizotia abyssinica Cass.-A multipurpose oil crop

“…A number of protocols for plant regeneration and in vitro flowering through tissue culture has been reported in the past in Guizotia abyssinica (Sarvesh et al 1996), Ocimum basilicum (Sudhakaran and Sivasankari 2002), Vernonia cinerea (Maheshwari and Kumar 2006), Anthemis xylopoda (Erdag and Emek 2009), Scoparia dulcis (Alieni et al 2011), Arachis paraguariensis (Aina et al 2012) and Bacopa chamaedryoides (Haque and Ghosh 2013b).…”