Photoperiod‐induced apoptosis in the male genital tract epithelia of the golden hamster

Carballada, Rosa; Jara, Marco; Esponda, Pedro

doi:10.1111/j.1365-2605.2006.00712.x

Cited by 6 publications

(2 citation statements)

References 32 publications

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“…Our results show the expression levels of casp3 to significantly increase in DD samples compared to the other regimes suggesting that microbial communities may increase the occurrence of apoptosis. This finding is in agreement with Carballada et al, which identified the presence of apoptotic cells in the epithelium of the epididymis, seminal vesicles, prostate and coagulating gland of the golden hamster (Mesocricetus auratus) following a short-day light regimen (8:16 LD cycle) [89].…”

Section: Discussionsupporting

confidence: 92%

Photoperiod Manipulation Affects Transcriptional Profile of Genes Related to Lipid Metabolism and Apoptosis in Zebrafish (Danio rerio) Larvae: Potential Roles of Gut Microbiota

et al. 2019

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Gut microbiota plays a fundamental role in maintaining host's health by controlling a wide range of physiological processes. Administration of probiotics and manipulation of photoperiod have been suggested as modulators of microbial composition and are currently undergoing an extensive research in aquaculture as a way to improve health and quality of farmed fish. However, our understanding regarding their effects on physiological processes is still limited. In the present study we investigated whether manipulation of photoperiod and/or probiotic administration was able to alter microbial composition in zebrafish larvae at hatching stage. Our findings show that probiotic does not elicit effects while photoperiod manipulation has a significant impact on microbiota composition. Moreover, we successfully predicted lipid biosynthesis and apoptosis to be modulated by microbial communities undergoing continuous darkness. Interestingly, expression levels of caspase 3 gene (casp3) and lipid-related genes (hnf4a, npc1l1, ppar, srebf1, agpat4 and fitm2) were found to be significantly overexpressed in dark-exposed larvae, suggesting an increase in the occurrence of apoptotic processes and a lipid metabolism impairment, respectively (p<0.05). Our results provide the evidence that microbial communities in zebrafish at early-life stages are not modulated by a short administration of probiotics and highlight the significant effect that the dark photoperiod elicits on zebrafish microbiota and potentially on health.Recently, Deaver et al., observed Ruminococcus torques, a bacterial species known to negatively affect gut barrier integrity, and Lactobacillus johnsonii, known to help maintaining the intestinal epithelial cell layer, to respectively increase and decrease their abundance in mice undergoing a 4-week period of constant 24 h light [17]. Thus, it is of paramount importance for the microbiota to follow regular diurnal oscillation in order to protect against homeostasis impairment and, consequently, diseases.

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Section: Discussionsupporting

confidence: 92%

Photoperiod Manipulation Affects Transcriptional Profile of Genes Related to Lipid Metabolism and Apoptosis in Zebrafish (Danio rerio) Larvae: Potential Roles of Gut Microbiota

et al. 2019

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“…In the case of the aged epididymis the apoptotic changes observed were related to a fall in testosterone and a discrete decrease in androgen receptors (Jara et al, 2004). Furthermore, recent experiments demonstrated that a photoperiod-induced apoptosis occurs in the epididymis of the golden hamster (Carballada et al, 2007).…”

Section: Introductionmentioning

confidence: 93%

Increase in Apoptosis and of the Stress Protein HSP70 in the Mouse Epididymis Produced by the Antiandrogen Flutamide

Obregón

Esponda

2009

Int. J. Morphol.

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The general aim of this paper was to characterize some changes induced by androgen receptors blockage in the epithelial cells of the mouse epididymis. The antiandrogen flutamide was injected (10 mg/Kg b.w.) to adult male mice which were sacrificed 24h. and 72h. after. Controls injected with the vehicle (corn oil) were sacrificed at the same intervals. Cryosections were made of the epididymides and examined by the TUNEL method for quantification of apoptosis and also using immunocytochemistry to visualize the expression of the stress protein HSP70. The highest indexes of apoptosis were observed in the caput epididymis after 72 h. and were of 7.40 cells/1000 in contrast to controls (0.21 cells/1000). HSP70 appeared particularly increased in the caput and cauda epididymis after 72 h. treatment. Results indicated that the blockage of androgen receptors induces apoptosis and a HSP70 expression in the principal epithelial cells of the mouse epididymis, and that these changes occur in a region-specific fashion.

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Seasonal variation of cell proliferation and apoptosis in the efferent ductules and epididymis of the Neotropical bat Artibeus lituratus (Chiroptera, Phyllostomidae)

Campolina-Silva

Hess

Oliveira

2019

General and Comparative Endocrinology

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The balance between cell proliferation and apoptosis is important for maintenance of male fertility, being influenced by a variety of stimuli including androgens and estrogens. However, studies concerning regulation of these processes along the male reproductive tract under physiological conditions are scarce. Therefore, in this study, we investigated the profile of cell proliferation and apoptosis in the efferent ductules and epididymis of the Neotropical bat Artibeus lituratus, a seasonal breeder that presents natural variation in components of the androgen and estrogen responsive systems along the circannual cycle. Low rates of cell proliferation and apoptosis were found in the efferent ductules and epididymis of A. lituratus during the reproductive period, as few epithelial cells were positive for MCM7 (proliferation marker) and cleaved caspase-3 or TUNEL (apoptosis markers). In contrast, during the regressive period, the rate of both proliferating and apoptotic cells was significantly higher in the epithelium lining the efferent ductules as well as throughout the epididymis. The increased proliferative activity at this phase was positively correlated with the expression of estrogen receptor alpha (ERα), whereas the variation in apoptosis appears to be unrelated to the local expression of androgen and estrogen receptors. Together, these data suggest that cell proliferation and apoptosis are differentially modulated in the efferent ductules and epididymis of A. lituratus during the annual reproductive cycle, and support the hypothesis that ERα may be important in preparing the male reproductive tract for sexual recrudescence.

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Photoperiod‐induced apoptosis in the male genital tract epithelia of the golden hamster

Cited by 6 publications

References 32 publications

Photoperiod Manipulation Affects Transcriptional Profile of Genes Related to Lipid Metabolism and Apoptosis in Zebrafish (Danio rerio) Larvae: Potential Roles of Gut Microbiota

Photoperiod Manipulation Affects Transcriptional Profile of Genes Related to Lipid Metabolism and Apoptosis in Zebrafish (Danio rerio) Larvae: Potential Roles of Gut Microbiota

Increase in Apoptosis and of the Stress Protein HSP70 in the Mouse Epididymis Produced by the Antiandrogen Flutamide

Seasonal variation of cell proliferation and apoptosis in the efferent ductules and epididymis of the Neotropical bat Artibeus lituratus (Chiroptera, Phyllostomidae)

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