Photoperiod Stress in Arabidopsis thaliana Induces a Transcriptional Response Resembling That of Pathogen Infection

Cortleven, Anne; Roeber, Venja M.; Frank, Manuel; Bertels, Jonas; Lortzing, Tobias; Beemster, Gerrit T.S.; Schmülling, Thomas

doi:10.3389/fpls.2022.838284

Cited by 8 publications

(8 citation statements)

References 95 publications

(154 reference statements)

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“…In agreement with our findings, a recent report revealed that SA-related responses are primed by photoperiodic stress (Cortleven et al, 2022). Considering that SA can antagonize ABA responses, we investigated whether SA is necessary for light-mediated prevention of water-soaked lesions.…”

Section: Resultssupporting

confidence: 94%

“…However, our results showing that it affects aROS production and SA-related responses, but not early PTI responses, suggest that constant light affects specific modules in plant immunity. At the same time, our results are consistent with a recent study showing that, under photoperiodic stress, the Arabidopsis transcriptional signature resembles that of a response to pathogens (Cortleven et al, 2022).…”

Section: Discussionsupporting

confidence: 93%

“…In agreement with our findings, a recent report revealed that SA-related responses are primed by photoperiodic stress (Cortleven et al, 2022).…”

Section: Salicylic Acid Is Required To Prevent Effector-mediated Indu...supporting

confidence: 94%

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Light prevents pathogen-induced aqueous microenvironments via potentiation of salicylic acid signaling

Lajeunesse

Roussin-Léveillée

Boutin

et al. 2022

Preprint

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Upon establishment of an infection, many plant pathogens induce an aqueous microenvironment in the extracellular space of their host, resulting in water-soaked lesions. In the case of Pseudomonas syringae (Pst), this is accomplished through the activity of water-soaking effectors that stimulate abscisic acid (ABA) production and signaling, which results in stomatal closure. This reduces transpiration and induces a microenvironment favorable for bacterial growth. Stomata are also highly sensitive to environmental conditions, including light and circadian rhythm. Here, we show that a period of darkness is required for water-soaking, and that a constant light regime abrogates the water-soaking activity of Pst effectors. Additionally, we show that constant light induces resistance against Pst and that this effect requires salicylic acid (SA). An increase in SA production upon infection under constant light did not affect effector-induced ABA signaling, but rather abrogated ABA's ability to induce stomatal closure. Indeed, under normal diurnal light regimes, application of a SA analog is sufficient to prevent the ability of the pathogen to induce stomatal closure and a water-rich niche in the apoplast. Our results provide a novel approach to interfering with a common virulence strategy, as well as providing a physiological mechanism by which SA functions in defense against certain pathogens.

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Section: Resultssupporting

confidence: 94%

Section: Discussionsupporting

confidence: 93%

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Light prevents pathogen-induced aqueous microenvironments via potentiation of salicylic acid signaling

Lajeunesse

Roussin-Léveillée

Boutin

et al. 2022

Preprint

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show abstract

“…In agreement with our findings, a recent report revealed that SArelated responses are primed by photoperiodic stress 33 . Considering that SA can antagonize ABA responses, we investigated whether SA is necessary for light-mediated prevention of water-soaked lesions.…”

Section: Pst Dc3000 Requires Darkness To Induce Water-soaking Lesionssupporting

confidence: 94%

Light prevents pathogen-induced aqueous microenvironments via potentiation of salicylic acid signaling

et al. 2023

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Many plant pathogens induce water-soaked lesions in infected tissues. In the case of Pseudomonas syringae (Pst), water-soaking effectors stimulate abscisic acid (ABA) production and signaling, resulting in stomatal closure. This reduces transpiration, increases water accumulation, and induces an apoplastic microenvironment favorable for bacterial growth. Stomata are sensitive to environmental conditions, including light. Here, we show that a period of darkness is required for water-soaking, and that a constant light regime abrogates stomatal closure by Pst. We find that constant light induces resistance to Pst, and that this effect requires salicylic acid (SA). Constant light did not alter effector-induced accumulation of ABA, but induced greater SA production, promoting stomatal opening despite the presence of ABA. Furthermore, application of a SA analog was sufficient to prevent pathogen-induced stomatal closure and water-soaking. Our results suggest potential approaches for interfering with a common virulence strategy, as well as providing a physiological mechanism by which SA functions in defense against pathogens.

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“… 5 Improved plant resistance was also observed when the light period the day prior to the Pst infection is extended from 8 h to 16–32 h as a consequence of photoperiod stress. 6 A 24 h pre-exposure of Arabidopsis to an extended or continuous light phase increases the ability for a strong apoplastic production of reactive oxygen species, boosts pathogen-driven salicylic acid accumulation and signaling, and reduces the capability of Pst for inducing so-called water-soaking leasions. 7 , 8 …”

Section: Short Communicationmentioning

confidence: 99%

Cold priming on pathogen susceptibility in the Arabidopsis eds1 mutant background requires a functional stromal Ascorbate Peroxidase

Schütte,

Baier,

Griebel

2024

Plant Signaling & Behavior

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24 h cold exposure (4°C) is sufficient to reduce pathogen susceptibility in Arabidopsis thaliana against the virulent Pseudomonas syringae pv. tomato ( Pst ) strain even when the infection occurs five days later. This priming effect is independent of the immune regulator Enhanced Disease Susceptibility 1 (EDS1) and can be observed in the immune-compromised eds1–2 null mutant. In contrast, cold priming-reduced Pst susceptibility is strongly impaired in knock-out lines of the stromal and thylakoid ascorbate peroxidases (sAPX/tAPX) highlighting their relevance for abiotic stress-related increased immune resilience. Here, we extended our analysis by generating an eds1 sapx double mutant. eds1 sapx showed eds1 -like resistance and susceptibility phenotypes against Pst strains containing the effectors avrRPM1 and avrRPS4. In comparison to eds1–2 , susceptibility against the wildtype Pst strain was constitutively enhanced in eds1 sapx . Although a prior cold priming exposure resulted in reduced Pst titers in eds1–2 , it did not alter Pst resistance in eds1 sapx . This demonstrates that the genetic sAPX requirement for cold priming of basal plant immunity applies also to an eds1 null mutant background.

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Photoperiod Stress in Arabidopsis thaliana Induces a Transcriptional Response Resembling That of Pathogen Infection

Cited by 8 publications

References 95 publications

Light prevents pathogen-induced aqueous microenvironments via potentiation of salicylic acid signaling

Light prevents pathogen-induced aqueous microenvironments via potentiation of salicylic acid signaling

Light prevents pathogen-induced aqueous microenvironments via potentiation of salicylic acid signaling

Cold priming on pathogen susceptibility in the Arabidopsis eds1 mutant background requires a functional stromal Ascorbate Peroxidase

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