Photoperiods induced the circRNA differential expression in the thyroid gland of OVX+E2 ewes

Wang, Wei; He, Xiaoyun; Di, Ran; Wang, Xiangyu; Chu, Mingxing

doi:10.3389/fendo.2022.974518

Cited by 3 publications

(2 citation statements)

References 67 publications

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“…These findings suggest that miR-370-3p may function as a regulator of early pregnancy establishment and maintenance in sheep [26]. RNA-seq analysis of the adrenal glands of STH sheep [27] with different FecB genotypes and thyroid tissues of Sunite sheep [28] with different photoperiods revealed miR-370-3p as the central element of the network. These findings suggest that miR-370-3p could potentially impact the reproductive process in sheep.…”

Section: Discussionmentioning

confidence: 76%

Thyroid Transcriptomics Revealed the Reproductive Regulation of miRNA in the Follicular and Luteal Phases in Small-Tail Han Sheep with Different FecB Genotypes

Chang,

He,

et al. 2023

Genes

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MicroRNA (miRNA) is a type of endogenous short−stranded ncRNA that influences many biological processes such as animal growth, development and metabolism. The thyroid gland is an important endocrine gland in sheep, and an increasing number of studies have shown that the thyroid gland plays an important role in animal reproduction, but the molecular mechanisms of the thyroid gland in sheep reproduction are poorly understood. In this study, RNA-seq was used to detect transcriptome expression patterns in the thyroid gland between the follicular phase (FP) and luteal phase (LP) in FecB BB (MM) and FecB ++ (ww) small-tail Han (STH) sheep, respectively, and to identify differentially expressed miRNAs (DEMs) associated with reproduction. Bioinformatic analysis of the target genes of these DEMs revealed that they can be enriched in multiple GO terms associated with the reproductive process in animals and in the KEGG signaling pathway. The miRNA–mRNA coexpression network revealed that oar-miR-133 and oar-miR-370-3p may play an important role in sheep reproduction. The results of the dual-luciferase reporter assay suggest a possible targeting relationship between novel-51 and TARBP2. These results provided a novel resource for elucidating regulatory mechanisms underlying STH sheep prolificacy.

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Section: Discussionmentioning

confidence: 76%

Thyroid Transcriptomics Revealed the Reproductive Regulation of miRNA in the Follicular and Luteal Phases in Small-Tail Han Sheep with Different FecB Genotypes

Chang,

He,

et al. 2023

Genes

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“…In honeybees, the reversible transition from a "mammalian" to a "foraging" behavioral phenotype is associated with genomic changes in methylation patterns [21]. In addition, it has been shown that specific non-coding RNAs can regulate candidate genes associated with seasonal estrus in sheep [22,23].…”

Section: Introductionmentioning

confidence: 99%

Photoperiod Induces the Epigenetic Change of the GNAQ Gene in OVX+E2 Ewes

Wang,

Du,

Chu

et al. 2023

IJMS

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GNAQ, a member of the alpha subunit encoding the q-like G protein, is a critical gene in cell signaling, and multiple studies have shown that upregulation of GNAQ gene expression ultimately inhibits the proliferation of gonadotropin-releasing hormone (GnRH) neurons and GnRH secretion, and ultimately affects mammalian reproduction. Photoperiod is a key inducer which plays an important role in gene expression regulation by affecting epigenetic modification. However, fewer studies have confirmed how photoperiod induces epigenetic modifications of the GNAQ gene. In this study, we examined the expression and epigenetic changes of GNAQ in the hypothalamus in ovariectomized and estradiol-treated (OVX+E2) sheep under three photoperiod treatments (short photoperiod treatment for 42 days, SP42; long photoperiod treatment for 42 days, LP42; 42 days of short photoperiod followed by 42 days of long photoperiod, SP-LP42). The results showed that the expression of GNAQ was significantly higher in SP-LP42 than in SP42 and LP42 (p < 0.05). Whole genome methylation sequencing (WGBS) results showed that there are multiple differentially methylated regions (DMRs) and loci between different groups of GNAQ. Among them, the DNA methylation level of DMRs at the CpG1 locus in SP42 was significantly higher than that of SP-LP42 (p < 0.01). Subsequently, we confirmed that the core promoter region of the GNAQ gene was located with 1100 to 1500 bp upstream, and the DNA methylation level of all eight CpG sites in SP42 was significantly higher than those in LP42 (p < 0.01), and significantly higher than those in SP-LP42 (p < 0.01), except site 2 and site 4 in the first sequencing fragment (p < 0.05) in the core promoter region. The expression of acetylated GNAQ histone H3 was significantly higher than that of the control group under three different photoperiods (p < 0.01); the acetylation level of sheep hypothalamic GNAQ genomic protein H3 was significantly lower under SP42 than under SP-LP42 (p < 0.05). This suggests that acetylated histone H3 binds to the core promoter region of the GNAQ gene, implying that GNAQ is epigenetically regulated by photoperiod through histone acetylation. In summary, the results suggest that photoperiod can induce DNA methylation in the core promoter region and histone acetylation in the promoter region of the GNAQ gene, and hypothesize that the two may be key factors in regulating the differential expression of GNAQ under different photoperiods, thus regulating the hypothalamus–pituitary–gonadal axis (HPGA) through the seasonal estrus in sheep. The results of this study will provide some new information to understand the function of epigenetic modifications in reproduction in sheep.

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Circular RNAs: characteristics, functions, mechanisms, and potential applications in thyroid cancer

Wu,

Yuan,

Zou

et al. 2023

Clin Transl Oncol

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Photoperiods induced the circRNA differential expression in the thyroid gland of OVX+E2 ewes

Cited by 3 publications

References 67 publications

Thyroid Transcriptomics Revealed the Reproductive Regulation of miRNA in the Follicular and Luteal Phases in Small-Tail Han Sheep with Different FecB Genotypes

Thyroid Transcriptomics Revealed the Reproductive Regulation of miRNA in the Follicular and Luteal Phases in Small-Tail Han Sheep with Different FecB Genotypes

Photoperiod Induces the Epigenetic Change of the GNAQ Gene in OVX+E2 Ewes

Circular RNAs: characteristics, functions, mechanisms, and potential applications in thyroid cancer

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