Photophysical and photodynamic properties of Pyronin Y in micellar media at different temperatures

Abstract

In this study, photophysics and photodynamical properties of Pyronin Y (PyY) in different liquid media were investigated. Interactions of PyY, which is a positively charged pigment compound pertaining to the xanthene derivatives with surfactants possessing distinct charges, were determined by using the molecular absorption and fluorescence spectroscopy techniques. It was observed that band intensities of absorption and fluorescence spectra belonging to PyY increase in proportion to the water when compared to three micelle systems, cetyltrimethylammonium bromide (CTAB), sodium dodecyl sulfate (SDS) and Triton X-100 (TX-100). This suggests that interactions in micelle systems are different from those in deionized water, and solvation and surface interactions modify. It is determined that the strongest interaction occurs between PyY dye and SDS, anionic surfactant, and this interaction arises from the electrostatic forces. Calculated photophysical parameters indicated that the microenvironment of PyY in SDS micelle is different to that of other systems. In temperature studies, it was reported that increasing the temperature of the samples increased non-radiative transitions. Steady-state fluorescence anisotropy values were calculated by using fluorescence intensities of PyY compound in pre-micellar, micellar and post-micellar systems. Once the PyY fluorescence probe is added to the surfactant containing solutions below the critical micelle concentrations, the measured anisotropy values were found to be low because the probe remains in the deionized water phase. When the surfactant concentration of the medium becomes closer to the critical micelle concentrations, the steady-state anisotropy value prominently increases. This is because of the restrictions on the rotational diffusion of the probe in micellar solution. It is observed that positively charged PyY shows a higher affinity to the negatively charged SDS compared with the positively charged CTAB and neutral TX-100 surfactants. This can be explained by Coulombic interactions.

Exploring Biological Interactions: A New Pyrazoline as a Versatile Fluorescent Probe for Energy Transfer and Cell Staining Applications

“…The overlap of the integrals J for the PYDP and dye(s) pair in SDS ranged from 9.03×10 −14 to 1.57×10 −15 . The Förster distance is usually between 2–6 nm [36] . In this study, ranged from 36.85 Å and 40.22 Å for FRET pairs, indicating that energy transfer from PYDP to the dyes is highly likely to occur.…”

“…The Förster distance is usually between 2–6 nm. [36] In this study, ranged from 36.85 Å and 40.22 Å for FRET pairs, indicating that energy transfer from PYDP to the dyes is highly likely to occur. FRET efficiency (E) is the fraction of photon energy absorbed by the donor and transmitted to the acceptor.…”

“…Dye solutions (1.0×10 −3 M) and PYDP solution (1.0×10 −4 M) were prepared as stock solutions in a 2 : 1 ethanol‐chloroform mixture and kept in dark. In this study, the SDS concentration was prepared as 8.5 mM, close to the critical micelle concentration (8.1 mM) [36] . While preparing the phosphate buffer solution, a PBS tablet was dissolved in 200 mL of deionized water using an ultrasonic bath.…”

Vibrationally Resolved Absorption, Fluorescence, and Preresonance Raman Spectroscopy of Isolated Pyronin Y Cation at 5 K

“…Pyronin Y (6-(dimethylamino)- N , N -dimethyl-3 H -xanthen-3-iminium chloride; [ PY ] + •Cl – ; also known as pyronin G) is a cationic organic dye having a planar heterocyclic structure with conjugated double bonds (as shown in Figure a). It is a well-known, bright green-yellow fluorophore and the most commonly utilized fluorescent probe among the pyronin family ([PY] + , pyronin B, and acridine red) . Since its first synthesis in 1894, , the fluorescent and selective RNA staining properties of pyronin Y and its synthetically tailored derivatives have been exploited in many applications, e.g., in histochemistry, , optoelectronics, , and microscopy. , As a result, pyronin Y has also been the subject of many experimental and theoretical studies to better understand its spectral properties, e.g., toward improving fluorescence quantum yields (Φ f ) and enhancing molar absorptivity by appropriate functionalization. − Despite this intense activity, a molecular-level description of the vibronic properties of this model fluorophore with high spectroscopic accuracy is still missing.…”

“…It is a wellknown, bright green-yellow fluorophore and the most commonly utilized fluorescent probe among the pyronin family ([PY] + , pyronin B, and acridine red). 5 Since its first synthesis in 1894, 6,7 the fluorescent and selective RNA staining properties of pyronin Y 8 and its synthetically tailored derivatives have been exploited in many applications, e.g., in histochemistry, 9,10 optoelectronics, 11,12 and microscopy. 13,14 As a result, pyronin Y has also been the subject of many experimental and theoretical studies to better understand its spectral properties, e.g., toward improving fluorescence quantum yields (Φ f ) and enhancing molar absorptivity by appropriate functionalization.…”