Photophysical Properties of Fluorescent Labels: A Meta‐Analysis to Guide Probe Selection Amidst Challenges with Available Data

Cavazos‐Elizondo, Daniela; Aguirre‐Soto, Alan

doi:10.1002/anse.202200056

Cited by 5 publications

(3 citation statements)

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“…At ϑ i = 45°, anticrossing energy ℏΩ of the cavity/EL exciton rhodamine system has been estimated to be ℏΩ EL = 330 meV. Additionally, as γ spp ( ϑ i ) = 110 meV (Table ) and γ EL = 0.28 meV achieved by accounting for the dissipation energy of the molecules (quantum yield:lifetime ratio), the low-energy mode is characterized by having a strong coupling, as demonstrated by the satisfied hierarchy and a coupling rate G 1 of 165. At ϑ i = 55°, the cavity/EH exciton system has an anticrossing energy ℏΩ EH of 800 meV and the dissipation energy of the molecules γ EH is 0.15 meV; therefore, because γ spp ( ϑ i ) = 150 meV (Table ), once again the strong coupling is demonstrated and the coupling rate is calculated as G 2 = 339.…”

Section: Resultsmentioning

confidence: 99%

Mapping the Optomechanical Coupling Mediated by Light among Mechanical Resonators Including Multilayer Cavity and Molecules

Simone

2022

ACS Appl. Opt. Mater.

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For a wide range of health applications, label-free sensing is essential, and microphotonics offers innovative technical solutions for pursuing important objectives. It has been shown that the strong light/matter coupling formed between a cavity and molecules through light excitation enhances biological and clinical applications by providing deep insights into molecular analysis. The multilayer cavity’s proposed architecture is meant to promote a robust interaction between light and matter. The top layer was made of silver Ag multishape features that, after being synthesized and characterized, were immobilized on the surface of a multilayer system. A layer of indium tin oxide (ITO) was flipped to produce two distinct layouts. An investigation of the mode behavior was conducted to describe the two layouts; experimental and numerical results point to a strong light/matter interaction by the ITO/SiO2/spacer/Ag design. For the characterization of light/matter coupling, a fluorophore was adsorbed on the surface; the anticrossing energy was then examined by integrating the experimental data with a three mechanical oscillator model. To show the system’s sensitivity, the analysis was carried out again using bovine serum albumin (BSA). The protein is water-soluble and exhibits an infrared absorption band (amide I), while also being active in the Raman region. In addition, it may consistently bind to Ag multishape features. The excellent sensitivity was demonstrated, enabling the use of image analysis to capture the surface-enhanced Raman scattering of BSA. In conclusion, the suggested sensing approach raises fresh possibilities for highly sensitive biomolecule detection method and encourages results when used as a fundamental sensing technique to investigate molecular patterns.

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Section: Resultsmentioning

confidence: 99%

Mapping the Optomechanical Coupling Mediated by Light among Mechanical Resonators Including Multilayer Cavity and Molecules

Simone

2022

ACS Appl. Opt. Mater.

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“…Luminescence methods such as fluorescence spectroscopy, microfluorometry, and fluorescence microscopy utilizing molecular and nanoscale luminescent reporters are broadly applied in the materials and life sciences. − Applications range from sensing and bioimaging to barcoding, solid-state lighting, and energy conversion. Performance parameters of luminescent reporters include the spectral position of the luminophore absorption and emission bands, their spectral widths and overlap, as well as fundamental spectroscopic quantities acting as measures for the absorption and emission efficiency such as the molar absorption coefficient or absorption cross section and the photoluminescence or fluorescence quantum yield (Φ f ) .…”

Section: Introductionmentioning

confidence: 99%

Interlaboratory Comparison on Absolute Photoluminescence Quantum Yield Measurements of Solid Light Converting Phosphors with Three Commercial Integrating Sphere Setups

Fiedler,

Frenzel,

Würth

et al. 2024

Anal. Chem.

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Scattering luminescent materials dispersed in liquid and solid matrices and luminescent powders are increasingly relevant for fundamental research and industry. Examples are luminescent nano- and microparticles and phosphors of different compositions in various matrices or incorporated into ceramics with applications in energy conversion, solid-state lighting, medical diagnostics, and security barcoding. The key parameter to characterize the performance of these materials is the photoluminescence/fluorescence quantum yield (Φf), i.e., the number of emitted photons per number of absorbed photons. To identify and quantify the sources of uncertainty of absolute measurements of Φf of scattering samples, the first interlaboratory comparison (ILC) of three laboratories from academia and industry was performed by following identical measurement protocols. Thereby, two types of commercial stand-alone integrating sphere setups with different illumination and detection geometries were utilized for measuring the Φf of transparent and scattering dye solutions and solid phosphors, namely, YAG:Ce optoceramics of varying surface roughness, used as converter materials for blue light emitting diodes. Special emphasis was dedicated to the influence of the measurement geometry, the optical properties of the blank utilized to determine the number of photons of the incident excitation light absorbed by the sample, and the sample-specific surface roughness. While the Φf values of the liquid samples matched between instruments, Φf measurements of the optoceramics with different blanks revealed substantial differences. The ILC results underline the importance of the measurement geometry, sample position, and blank for reliable Φf data of scattering the YAG:Ce optoceramics, with the blank’s optical properties accounting for uncertainties exceeding 20%.

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“…Samples were allowed to equilibrate for 2 min before each measurement. ATTO 520 dye used for the determination of relative quantum yields 40 was purchased from ATTO-TEC GmbH (Siegen, Germany).…”

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confidence: 99%

New Thiazole Orange Derivatives for Improved Fluorescence Signaling of DNA FIT Probes

Homer

Seitz

2023

Synthesis

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Forced intercalation probes (FIT-probes) probes are nucleic acid probes in which an intercalator dye of the thiazole orange (TO) family serves as a surrogate nucleobase. Hybridization of FIT probes is accompanied by enhancements of fluorescence. Looking for ways to increase turn-on and brightness of fluorescence, we herein report the synthesis of new fluorogenic base surrogates. In total, 9 different TO derivatives were introduced into FIT probes. Fluorescence measurements in 6 different sequence revealed that substitution at both the quinoline and the benzothiazole part affects fluorescence turn on upon hybridization and brightness of probe-target duplexes. A TO derivative containing a tricyclic benzothiazole provided FIT probes signaling hybridization by up to 19-fold enhancement of fluorescence. Improved fluorescence quantum yields (Φds up to 0.53) and a high extinction coefficients (ε518 up to 91.000∙M−1∙cm−1) make this dye an interesting, and in some sequences superior alternative to the canonical thiazole orange used previously in FIT probes.

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Photophysical Properties of Fluorescent Labels: A Meta‐Analysis to Guide Probe Selection Amidst Challenges with Available Data

Cited by 5 publications

References 0 publications

Mapping the Optomechanical Coupling Mediated by Light among Mechanical Resonators Including Multilayer Cavity and Molecules

Mapping the Optomechanical Coupling Mediated by Light among Mechanical Resonators Including Multilayer Cavity and Molecules

Interlaboratory Comparison on Absolute Photoluminescence Quantum Yield Measurements of Solid Light Converting Phosphors with Three Commercial Integrating Sphere Setups

New Thiazole Orange Derivatives for Improved Fluorescence Signaling of DNA FIT Probes

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