Photophysical properties of laser dyes: picosecond laser flash photolysis studies of Rhodamine 6G, Rhodamine B and Rhodamine 101

Beaumont, Paul C.; Johnson, David G.; Parsons, Barry J.

doi:10.1039/ft9938904185

Cited by 76 publications

(57 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For rhodamine 640, solution-phase lifetimes between 3.9 and 4.9 ns have been reported [46,48,65]. For rhodamine 575, the range of published solution-phase lifetimes is 3.8-4.16 ns [45,66].…”

Section: Discussionmentioning

confidence: 93%

See 1 more Smart Citation

Fluorescence lifetimes of rhodamine dyes in vacuo

Nagy

Talbot

Czar

et al. 2012

Journal of Photochemistry and Photobiology A: Chemistry

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 93%

“…Numerous investigations of their spectroscopic properties in the condensed phase exist [43][44][45][46][47][48][49][50][51]. The structures of the rhodamine dyes used in this study are shown in Scheme 1.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence lifetimes of rhodamine dyes in vacuo

Nagy

Talbot

Czar

et al. 2012

Journal of Photochemistry and Photobiology A: Chemistry

View full text Add to dashboard Cite

“…The lifetime was found to be 4.4 ns, which is in accordance with the literature. [42,43] Next, the Ac-TZ14011 peptide (7) was conjugated to Ir III complexes 4-6 via the amine functionality of d-Lys8 by using BOP as coupling reagent (Scheme 1). The conjugated Ir III complexes were purified by HPLC and characterized by mass spectrometry.…”

Section: Synthesis and Photophysical Propertiesmentioning

confidence: 99%

Peptide‐Functionalized Luminescent Iridium Complexes for Lifetime Imaging of CXCR4 Expression

et al. 2011

View full text Add to dashboard Cite

The chemokine receptor 4 (CXCR4) is over-expressed in 23 types of cancer in which it plays a role in, among others, the metastatic spread. For this reason it is a potential biomarker for the field of diagnostic oncology. The antagonistic Ac-TZ14011 peptide, which binds to CXCR4, has been conjugated to luminescent iridium dyes to allow for CXCR4 visualization. The iridium dyes are cyclometalated octahedral iridium(III) 2-phenylpyridine complexes that can be functionalized with one, two or three targeting Ac-TZ14011 peptides. Confocal microscopy and fluorescence lifetime imaging microscopy (FLIM) showed that the peptide-iridium complex conjugates can be used to visualize CXCR4 expression in tumor cells. The CXCR4 receptor affinity and specific cell binding of the mono-, di- and trimeric peptide derivatives were assessed by using flow cytometry. The three derivatives possessed nanomolar receptor affinity and could distinguish between cell lines with different CXCR4 expression levels. This yields the first example of a neutral iridium(III) complex functionalized with peptides for FLIM-based visualization of a cancer associated membrane receptor.

show abstract

“…A peak ion density of n p Ϸ 2 ϫ 10 8cm Ϫ3 is calculated for N Ϸ 3 ϫ 10 4 Rhodamine 640 ions trapped at q z ϭ 0.61 and T ϭ 300 K. The interaction volume is estimated from ␦V Ϸ A2 r for a beam focal diameter d Ϸ 150 m and HWHM of the radial cloud distribution r Ϸ 0.04 cm. In addition, using parameters appropriate for these experimental conditions I/I s Ϸ 1.7, ␦⍀/4 Ϸ 5 ϫ 10 Ϫ3 , f ϭ 3.5 ns, p Ϸ 15 ns, QY ϳ 0.95 [30], and T(1 Ϫ R) Ϸ 0.26 yields ͗N hv ͘ Ϸ 6 photons per laser pulse.…”

Section: A Fluorescence From Space Charge Limited Ion Densitymentioning

confidence: 99%

Pulsed fluorescence measurements of trapped molecular ions with zero background detection

Khoury¹,

Rodriguez‐Cruz²,

Parks³

2002

J Am Soc Mass Spectrom

View full text Add to dashboard Cite

Sensitive methods have been developed to measure laser-induced fluorescence from trapped ions by reducing the detection of background scattering to zero levels during the laser excitation pulse. The laser beam diameter has been reduced to ϳ150 m to eliminate scattering on trap apertures and the resulting laser-ion interaction is limited to a volume of ϳ10 Ϫ5 cm 3 which is ϳ0.03-0.15 of the total ion cloud volume depending on experimental conditions. The detection optics collected fluorescence only from within the solid angle defined by laser-ion interaction volume. Rhodamine 640 and Alexa Fluor 350 ions, commonly used as fluorescence resonance energy transfer (FRET) fluorophores, were generated in the gas phase by using electrospray ionization and injected into a radiofrequency Paul trap where they were stored and exposed to Nd:YAG laser pulses at 532 and 355 nm for times up to 10 m. Fluorescence emitted by these ions was investigated for several trap q z values and ion cloud temperatures. Analysis of photon statistics indicated an average of ϳ10 photons were incident on the PMT detector per 15 ns pulse for ϳ10 3 trapped ions in the interaction volume. Fluorescence measurements displayed a dependence on trapped ion number which were consistent with calculations of the space charge limited ion density. To investigate the quantitative capability of these fluorescence techniques, the laser-induced fragmentation of trapped Alexa I on traps provide a controlled environment in which fluorescence measurements can be performed on an ensemble of ions over timescales sufficient to consider small ion numbers and slow reaction rates. Laserinduced fluorescence measurements of trapped ions have been used in spectroscopic studies of atomic structure [1][2][3][4], the vibrational spectra of small molecules [5][6][7][8], as well as to characterize the dynamic cooling [9] and crystallization of atomic ions [10 -12].This paper introduces techniques that eliminate the detection of laser background scattering on trap apertures and internal surfaces allowing measurements to achieve both high sensitivity and large dynamic range. In previous pulsed laser measurements of molecular fluorescence collected from trapped species (discussed in references [5][6][7][8]), the radiative lifetimes were sufficiently long to allow collection of fluorescence radiation after the laser pulse. This greatly simplified methods to reduce the detection of background resulting from scattered laser radiation. However, experiments described in this paper apply the Nd:YAG pulsed laser to excite strongly emitting molecules having fluorescence lifetimes shorter than the laser pulse. This required the reduction of background laser scattering during laser excitation, a more demanding constraint.Techniques described in this paper will help to extend fluorescence measurements as an in situ probe of trapped ion properties including chemical identity, the growth and decay of specific ion populations, ion spectroscopy. Fluorescence emission from trapped ions also presen...

show abstract

Photophysical properties of laser dyes: picosecond laser flash photolysis studies of Rhodamine 6G, Rhodamine B and Rhodamine 101

Cited by 76 publications

References 13 publications

Fluorescence lifetimes of rhodamine dyes in vacuo

Fluorescence lifetimes of rhodamine dyes in vacuo

Peptide‐Functionalized Luminescent Iridium Complexes for Lifetime Imaging of CXCR4 Expression

Pulsed fluorescence measurements of trapped molecular ions with zero background detection

Contact Info

Product

Resources

About