Photoprotective activity of geopropolis produced by Melipona subnitida (Apidae, Meliponinae) in the semiarid of the Brazilian Northeast

Abstract

Although geopropolis has been the subject of many chemical and pharmacological studies, there are few studies investigating the photoprotective activity of formulations containing propolis. Thus, we investigated in vivo the photoprotective efficacy of the cream containing geopropolis extract by macroscopic and histological evaluation of the skin of Wistar rats subjected to ultraviolet radiation (UVB). We also evaluated the chemical composition of hydroethanolic extract using the HPLC-DADESI-MS/MS technique, as well as antioxidant activity by the photocolorimetric method of free radical DPPH (2.2-diphenyl-1-picrylhydrazine) and cytotoxic activity by the in vitro MTT quantitative method [brometo de 3- (4.5dimetiltiazol-2-il)-2.5-difeniltetrazolio]. The extract had a varied chemical composition, 29 different phenolic compounds being detected, distributed between phenols and flavonoids, the latter being represented by chalcones, flavones and flavonols. The highest percentages of DPPH inhibition e o baixo valor de IC50 indicaram que o extrato apresentou alta atividade antioxidante. The hydroethanolic extract did not exert cytotoxic effects since high percentages of viability of L929 fibroblasts were observed after incubation for 72 hours at different concentrations of the extract. On skin submitted to cream application containing of geopropolis extract and the irradiation with UVB did not occur macroscopic and histological lesions. Thus, we concluded that the cream containing of geopropolis extract produced by Melipona subnitida was able to protect the skin from lesions induced by UVB irradiation, thus demonstrating photoprotective effect.

Cytotoxicity and Biological Activities of Geopopolis Extract from the Stingless bee (Melipona scutellaris) in Isolates of Staphylococcus aureus

“…In this assay, the cytotoxic potential of EEGP against HaCaT cells at 24 h was evaluated, showing a reduction in cell viability at all three tested concentrations, with indices of 67.4 % at MICx4. Correlating with assays conducted by Batista et al, [40] the percentage of HaCaT keratinocyte viability at the highest tested concentration was above 60 %. In the experiments of Bomfim de Barros et al, [23] tests using α-Pinene against HaCaT cells, after 48h of treatment, demonstrated viability above 50 % at MIC, being cytotoxic only at MICx4.…”

Chemical profile, antioxidant and photoprotective activities of essential oil and crude extracts of Algerian Thymus serpyllum

“…The sunburn, skin inflammation and skin cancer may appear as a result of regular exposure to UV radiation. These UV rays can promote photochemical reactions that induce the generation of free radicals such as O2 -, HOO -, and OH· (Batista et al 2021). Some studies are in quest for natural sunscreen products to reverse and block these UV radiations.…”

Presença de compostos fenólicos em extrato glicólico de própolis e sua eficácia na proteção frente a radiação UV

“…O autor não Brazilian Journal of Health Review, Curitiba, v. 6, n. 5, p. 25310-25322, sep/oct., 2023 considerou este aumento como significativo. Por outro lado, no artigo publicado porBatista et al, (2021) observou-se um aumento no (FPS) de uma base creme após a incorporação do extrato hidroalcóolico de própolis, elevando o (FPS) in vitro do creme de 8,32 para 20,13.…”