Photoprotective effects of 2S,3R-6-methoxycarbonylgallocatechin isolated from Anhua dark tea on UVB-induced inflammatory responses in human keratinocytes

Kim, Donghwa; Hu, Ruoci; Fan, Yanhua; Xu, Yongnan; Park, Hyen Joo; Lee, Sang Kook

doi:10.1016/j.jphotobiol.2019.111704

Cited by 23 publications

(14 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…UVB irradiation was performed as described previously [30]. Briefly, HaCaT cells were plated in 60 mm diameter dishes at a density of 22 × 10 4 cells/well.…”

Section: Uvb Irradiationmentioning

confidence: 99%

Photoprotective Activity of Topsentin, A Bis(Indole) Alkaloid from the Marine Sponge Spongosorites genitrix, by Regulation of COX-2 and Mir-4485 Expression in UVB-Irradiated Human Keratinocyte Cells

et al. 2020

Self Cite

View full text Add to dashboard Cite

Skin is an important barrier to protect the body from environmental stress. However, exposure to ultraviolet radiation (UV) and various environmental oxidative stresses can cause skin inflammation. Cyclooxygenase-2 (COX-2) is an inducible enzyme that mediates the formation of prostaglandin E2 (PGE2) against internal and external inflammatory stimulations. Therefore, the inhibition of COX-2 is an important approach to maintain skin health and prevent skin inflammation and carcinogenesis. Topsentin, a bis(indolyl)imidazole alkaloid isolated from the marine sponge Spongosorites genitrix, has been reported to exhibit anti-tumor and anti-microbial activities. However, the effect of topsentin on skin inflammation and its underlying molecular mechanism has not been elucidated. In the present study, we identified the photoprotective effects of topsentin on UVB irradiated human epidermal keratinocyte HaCaT cells. Topsentin suppresses COX-2 expression and its upstream signaling pathways, AP-1 and MAPK. Furthermore, topsentin inhibits miR-4485, a new biomarker selected from a microarray, and its target gene tumor necrosis factor alpha induced protein 2 (TNF-α IP2). The photoprotective effect of topsentin was also confirmed in a reconstructed human skin model. These findings suggest that topsentin may serve as a potential candidate for cosmetic formulations with skin inflammatory-mediated disorder.

show abstract

“…UVB irradiation was performed as described previously [30]. Briefly, HaCaT cells were plated in 60 mm diameter dishes at a density of 22 × 10 4 cells/well.…”

Section: Uvb Irradiationmentioning

confidence: 99%

Photoprotective Activity of Topsentin, A Bis(Indole) Alkaloid from the Marine Sponge Spongosorites genitrix, by Regulation of COX-2 and Mir-4485 Expression in UVB-Irradiated Human Keratinocyte Cells

et al. 2020

Self Cite

View full text Add to dashboard Cite

show abstract

“…Many examples show their activity in different in vitro models. In particular, Kim et al studied the antioxidant properties of a specific polyphenol isolated from Anhua dark tea [41], 2S,3R-6-methoxycarbonylgallocatechin (MCGE), in the same model of HEK293 cells we described in the paper. Indeed, dark tea is known for its abundancy of secondary metabolites such as flavonoids and catechins.…”

Section: Radical Scavenging Antioxidant and Anti-inflammatory Activitiesmentioning

confidence: 99%

Liquid Chromatography–High-Resolution Mass Spectrometry (LC-HRMS) Profiling of Commercial Enocianina and Evaluation of Their Antioxidant and Anti-Inflammatory Activity

et al. 2022

View full text Add to dashboard Cite

Enocianina is an anthocyanin-rich extract obtained from grape pomace. It is widely used as a colorant in the food industry and, in addition to anthocyanins, it also contains a variety of polyphenols. To understand whether enocianina, besides its coloring effect, may offer potential health benefit applications, we aimed to fully characterize the profile of four commercial enocianinas and assess their radical scavenging, enzymatic, antioxidant, and anti-inflammatory activities. LC-ESI-MS/MS analysis identified 90 phytochemicals. The relative content of each anthocyanin was assessed by a semi-quantitative analysis, with malvidin derivatives being the most abundant. UV-VIS spectroscopy detected total amounts of polyphenols and anthocyanins of 23% and 3.24%, respectively, indicating that anthocyanins represent a minor fraction of total polyphenols. Multiple linear regression analysis indicated that the radical scavenging activity is related to the total polyphenol content and not to anthocyanins. All four enocianinas dose-dependently activate Nrf2, and such activity was correlated with catechol-containing polyphenol content. Finally, all enocianinas showed dose-dependent anti-inflammatory activity, which at the highest concentrations tested was closely related to the total polyphenol content and was explained by radical scavenging, Nrf2 activation, and other mechanisms related to the polyphenolic components.

show abstract

“…In vitro and in vivo studies show that tea intake ( C. sinensis ) is associated with biological activities in the human body, through the effects of decreasing reactive oxygen species (ROS) and oxidative damage to DNA ( Kapoor, Sugita, Nishimura, Sudo, & Okubo, 2018 ). Biological effects are associated with reducing the risk of developing and treating cancerous tumors ( Bhattacharya et al, 2020 , Haghparasti and Mahdavi Shahri, 2018 , Rawangkan et al, 2018 , Shin et al, 2018 ) attenuating effect on inflammatory processes through signaling pathways ( Fechtner et al, 2017 , Kim et al, 2020 , Su et al, 2019 ), protection against cardiovascular disease ( Chung et al, 2020 ), hepatoprotective actions ( Cao et al, 2020 , Stefan et al, 2019 ), prevention and treatment of diabetes mellitus ( Cheng et al, 2020 , Meng et al, 2019 ), neuroprotection and immune modulation ( Schimidt et al, 2018 , Sharma et al, 2017 ), in addition to having recently filed a response against SARS-Cov-2 ( Zhu & Xie, 2020 ).…”

Section: Applications Of Phenolic Compounds Of Teas In Food Productsmentioning

confidence: 99%

Processing, chemical signature and food industry applications of Camellia sinensis teas: An overview

et al. 2021

View full text Add to dashboard Cite

Photoprotective effects of 2S,3R-6-methoxycarbonylgallocatechin isolated from Anhua dark tea on UVB-induced inflammatory responses in human keratinocytes

Cited by 23 publications

References 48 publications

Photoprotective Activity of Topsentin, A Bis(Indole) Alkaloid from the Marine Sponge Spongosorites genitrix, by Regulation of COX-2 and Mir-4485 Expression in UVB-Irradiated Human Keratinocyte Cells

Photoprotective Activity of Topsentin, A Bis(Indole) Alkaloid from the Marine Sponge Spongosorites genitrix, by Regulation of COX-2 and Mir-4485 Expression in UVB-Irradiated Human Keratinocyte Cells

Liquid Chromatography–High-Resolution Mass Spectrometry (LC-HRMS) Profiling of Commercial Enocianina and Evaluation of Their Antioxidant and Anti-Inflammatory Activity

Processing, chemical signature and food industry applications of Camellia sinensis teas: An overview

Contact Info

Product

Resources

About