Photoreactive interpenetrating network of hyaluronic acid and Puramatrix as a selectively tunable scaffold for neurite growth

Khoshakhlagh, Parastoo; Moore, Michael J.

doi:10.1016/j.actbio.2015.01.014

Cited by 49 publications

(33 citation statements)

References 41 publications

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“…To further improve biofunctionality of MeHA hydrogel, incorporating other organic or inorganic components into it can enhance its mechanical and chemical properties. For nerve repair, combination of MeHA hydrogel with puramatrix peptide hydrogel could improve neurite extension or outgrowth [111]. Moreover, MeHA hydrogels have been electrospun into aligned nanofibers and incorporated with poly-lactic-co-glycolic microspheres to control the release of nervous growth factor for peripheral nerve repair and regeneration [105].…”

Section: Synthesis Properties and Recent Biomedical Applications Of Phmentioning

confidence: 99%

Recent Advances in Photo-Crosslinkable Hydrogels for Biomedical Applications

et al. 2019

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Photo-crosslinkable hydrogels have recently attracted significant scientific interest. Their properties can be manipulated in a spatiotemporal manner through exposure to light to achieve the desirable functionality for various biomedical applications. This review article discusses the recent advances of the most common photo-crosslinkable hydrogels, including poly(ethylene glycol) diacrylate, gelatin methacryloyl and methacrylated hyaluronic acid, for various biomedical applications. We first highlight the advantages of photopolymerization and discuss diverse photosensitive systems used for the synthesis of photo-crosslinkable hydrogels. We then introduce their synthesis methods and review their latest state of development in biomedical applications, including tissue engineering and regenerative medicine, drug delivery, cancer therapies and biosensing. Lastly, the existing challenges and future perspectives of engineering photo-crosslinkable hydrogels for biomedical applications are briefly discussed.

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Section: Synthesis Properties and Recent Biomedical Applications Of Phmentioning

confidence: 99%

Recent Advances in Photo-Crosslinkable Hydrogels for Biomedical Applications

et al. 2019

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“…We have previously developed a facile and rapid technique that uses digital projection photolithography to fabricate desired 3D hydrogels and molecular micropatterns . We have used this method for developing models to investigate neurite growth and guidance, and as preclinical physiological models for drug development . In this report, we have used this approach to photomicropattern functionalized dextran and encapsulate neurons to study and compare their viability and neurite outgrowth potential upon UVA or visible light irradiation, in a manner in which the irradiation spectrum is the only variable.…”

Section: Introductionmentioning

confidence: 99%

Comparison of visible and UVA phototoxicity in neural culture systems micropatterned with digital projection photolithography

Khoshakhlagh

Bowser

Brown

et al. 2018

J Biomedical Materials Res

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Photopolymerization provides a favorable method for hydrogel formation due to its simplicity, convenience, and versatility. However, the light exposure required to initiate photopolymerization is known to have a cytotoxic effect on encapsulated cells. Here, a 3D in vitro model of the nervous system microenvironment, micropatterned through the use of digital projection photolithography using a single hydrogel formulation that cross-links similarly under ultraviolet A (UVA, 315-400 nm) and visible light (400-700 nm) exposure, is presented. This setup allowed for the investigation of neuronal responses to different light wavelengths and exposure times during photoencapsulation, while ruling out effects due to the hydrogel formulation or photoinitiators used. Cellular studies-including neurite viability, DNA fragmentation, and neurite outgrowth for both UVA and visible light irradiation, the most common spectra used in biological photomicropatterning applications-were performed to assess the effect of light source on neuronal cultures. These studies indicated that while cell death occurs after exposure to either spectrum, visible light was less phototoxic than UVA, when using comparable levels of irradiation, and interestingly, glial cells were more susceptible to phototoxicity than neuronal cells. Thus, while utilizing visible light for micropatterning and cell encapsulation for nervous system applications is beneficial, it is helpful to keep the light exposure low to ensure optimal neuronal survival and growth.

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“…Studies with a 3D scaffold of hyaluronic acid and PuraMatrix™ showed that when methacrylation of hyaluronic acid was decreased, neurite outgrowth from dorsal root ganglia (DRG) explants was more prominent. Methacrylation of hyaluronic acid causes degradation of crosslinking which corresponds with a certain degree of permeability (Khoshakhlagh and Moore, ). To ensure permeability in low elastic matrices, bio‐active hydrogels with microchannels were produced without changing the rigidity of the gel (Lee et al, ).…”

Section: Scaffold Requirementsmentioning

confidence: 99%

Building an Artificial Stem Cell Niche: Prerequisites for Future 3D‐Formation of Inner Ear Structures—Toward 3D Inner Ear Biotechnology

Groot

Sliedregt

Benthem

et al. 2019

The Anatomical Record

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In recent years, there has been an increased interest in stem cells for the purpose of regenerative medicine to deliver a wide range of therapies to treat many diseases. However, two‐dimensional cultures of stem cells are of limited use when studying the mechanism of pathogenesis of diseases and the feasibility of a treatment. Therefore, research is focusing on the strengths of stem cells in the three‐dimensional (3D) structures mimicking organs, that is, organoids, or organ‐on‐chip, for modeling human biology and disease. As 3D technology advances, it is necessary to know which signals stem cells need to multiply and differentiate into complex structures. This holds especially true for the complex 3D structure of the inner ear. Recent work suggests that although other factors play a role, the extracellular matrix (ECM), including its topography, is crucial to mimic a stem cell niche in vitro and to drive stem cells toward the formation of the tissue of interest. Technological developments have led to the investigation of biomaterials that closely resemble the native ECM. In the fast forward moving research of organoids and organs‐on‐chip, the inner ear has hardly received attention. This review aims to provide an overview, by describing the general context in which cells, matrix and morphogens cooperate in order to build a tissue, to facilitate research in 3D inner ear technology. Anat Rec, 303:408–426, 2020. © 2019 The Authors. The Anatomical Record published by Wiley Periodicals, Inc. on behalf of American Association of Anatomists.

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Photoreactive interpenetrating network of hyaluronic acid and Puramatrix as a selectively tunable scaffold for neurite growth

Cited by 49 publications

References 41 publications

Recent Advances in Photo-Crosslinkable Hydrogels for Biomedical Applications

Recent Advances in Photo-Crosslinkable Hydrogels for Biomedical Applications

Comparison of visible and UVA phototoxicity in neural culture systems micropatterned with digital projection photolithography

Building an Artificial Stem Cell Niche: Prerequisites for Future 3D‐Formation of Inner Ear Structures—Toward 3D Inner Ear Biotechnology

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