Photoreceptor Activity Contributes to Contrasting Responses to Shade in Cardamine and Arabidopsis Seedlings

Molina-Contreras, Maria José; Paulišić, Sandi; Then, Christiane; Moreno‐Romero, Jordi; Pastor-Andreu, Pedro; Morelli, Luca; Roig‐Villanova, Irma; Jenkins, Huw; Hallab, Asis; Gan, Xiangchao; Gómez-Cádenas, Aurelio; Tsiantis, Miltos; Rodríguez‐Concepción, Manuel; Martínez‐Garcia, Jaime F.

doi:10.1105/tpc.19.00275

Cited by 31 publications

(79 citation statements)

References 66 publications

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“…scant elongation under low light), the plasticity of some characteristics, especially the morphological characteristics of optimizing light capture, can be high in these plants (Smith, 1982;Valladares and Niinemets, 2008). Cardamine hirsuta is a close relative of Arabidopsis thaliana, and it is suggested that the lack of a shade-induced hypocotyl elongation response in C. hirsuta results from the enhanced repressor activity of the phytochrome A photoreceptor (Molina-Contreras et al, 2019). Exploitation of the molecular basis of shade-avoidance and shade-tolerance to improve crop yield and quality is of considerable importance for highdensity cultivation.…”

Section: Discussionmentioning

confidence: 99%

Progress of Research on the Regulatory Pathway of the Plant Shade-Avoidance Syndrome

et al. 2020

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When subject to vegetational shading, shade-avoiding plants detect neighbors by perceiving reduced light quantity and altered light quality. The former includes decreases in the ratio of red to far-red wavelengths (low R:FR) and low blue light ratio (LBL) predominantly detected by phytochromes and cryptochromes, respectively. By integrating multiple signals, plants generate a suite of responses, such as elongation of a variety of organs, accelerated flowering, and reduced branching, which are collectively termed the shade-avoidance syndrome (SAS). To trigger the SAS, interactions between photoreceptors and phytochrome-interacting factors are the general switch for activation of downstream signaling pathways. A number of transcription factor families and phytohormones, especially auxin, gibberellins, ethylene, and brassinosteroids, are involved in the SAS processes. In this review, shade signals, the major photoreceptors involved, and the phenotypic characteristics of the shade-intolerant plant Arabidopsis thaliana are described in detail. In addition, integration of the signaling mechanisms that link photoreceptors with multiple hormone signaling pathways is presented and future research directions are discussed.

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Section: Discussionmentioning

confidence: 99%

Progress of Research on the Regulatory Pathway of the Plant Shade-Avoidance Syndrome

et al. 2020

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“…and XTR7, known to be direct PIF target genes in A. thaliana, was strongly and rapidly enhanced in chfr1 and RNAi-HFR1 seedlings (Figure 1c, d). More importantly, rapid shade-induced expression was globally attenuated in Ch WT compared to At WT seedlings 26 .…”

Section: Discussionmentioning

confidence: 95%

“…In particular, the identification and characterization of the C. hirsuta phyA-deficient slender in shade 1 (sis1) mutant indicated that differential features of this photoreceptor in A. thaliana and C. hirsuta could explain their differential response to shade. Thus, stronger phyA activity in C. hirsuta wild-type plants resulted in a suppressed hypocotyl elongation response when exposed to low or very low R:FR 26 . These approaches indicated that the implementation of shade avoidance and shade tolerance involved the participation of shared genetic components.…”

Section: Introductionmentioning

confidence: 90%

“…Exposure of A. thaliana wild-type (At WT ) and Ch WT seedlings to low R:FR induces a rapid increase in the expression of various direct target genes of PIFs, including PIF3-LIKE 1 (PIL1), YUC8 and XTR7 (Figure 1c, d) [26][27][28] . The shade-induced expression of these genes was significantly higher in RNAi-HFR1 and chfr1 mutant lines compared to Ch WT (Figure 1c, d), indicating that ChHFR1 might repress shade-triggered hypocotyl elongation in part by downregulating the rapid shade-induced expression of these genes in C. hirsuta, as it was observed with AtHFR1 in A. thaliana seedlings 23 .…”

Section: Hfr1 Is Required For the Shade Tolerance Habit Of C Hirsutamentioning

confidence: 99%

“…To test if the lack of elongation of Ch WT hypocotyls in response to shade was caused by higher levels of ChHFR1 expression in this species, we used primer pairs that amplify HFR1 (Supplemental Figure 2a) and three housekeeping genes (EF1α, SPC25, YLS8) in both species 26 . As expected, expression of HFR1 was induced in shade-treated seedlings of both species, in agreement with the presence of canonical PIF-binding sites (G-box, CACGTG) in the HFR1 promoters 23,29 (Supplemental Figure 3a).…”

Section: Hfr1 Expression Is Higher In C Hirsuta Than In a Thaliana mentioning

confidence: 99%

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Adaptation to plant shade relies on rebalancing the transcriptional activity of the PIF-HFR1 regulatory module

Paulišić

Then

Alary

et al. 2020

Preprint

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Shade caused by the proximity of neighboring vegetation triggers a set of acclimation responses to either avoid or tolerate shade. Comparative analyses between the shade avoider Arabidopsis thaliana and the shade tolerant Cardamine hirsuta, revealed a role for the atypical basic-helix-loop-helix LONG HYPOCOTYL IN FR 1 (HFR1) in maintaining the shade-tolerance in C. hirsuta, inhibiting hypocotyl elongation in shade and constraining expression profile of shade induced genes. We showed that C. hirsuta HFR1 protein is more stable than its A. thaliana counterpart, contributing to enhance its biological activity. The enhanced HFR1 activity is accompanied by an attenuated PHYTOCHROME INTERACTING FACTOR (PIF) activity in C. hirsuta. As a result, the PIF-HFR1 module is imbalanced, causing a reduced PIF activity and attenuating other PIF-mediated responses such as warm temperature-induced hypocotyl elongation (thermomorphogenesis) and dark-induced senescence. By this mechanism and that of the already-known of phytochrome A photoreceptor, plants might ensure to properly adapt and thrive in habitats with disparate light amounts.

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Evolution of shade tolerance is associated with attenuation of shade avoidance and reduced phenotypic plasticity in North American milkweeds

Coverdale

Agrawal

2021

American J of Botany

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Premise: Mismatches between light conditions and light-capture strategy can reduce plant performance and prevent colonization of novel habitats. Although light-capture strategies tend to be highly conserved among closely related species, evolutionary transitions from shaded to unshaded habitats (and vice versa) occur in numerous plant lineages. Methods: We combined phylogenetic approaches with field and greenhouse experiments to investigate evolutionary constraints on light-capture strategy in North American milkweeds (genus Asclepias) and to determine whether colonization of shaded habitats in this heliophilic clade is associated with reduced plasticity and attenuation of the shade avoidance response. Results: Colonization of shaded habitats has occurred at least 10 times in this genus, including at least once in each major North American clade. Evolutionary transitions between habitats exhibit strong directional bias, with shifts from full-sun to shaded habitats occurring at least three times as often as the opposite transition. In field and greenhouse experiments, sun species responded to shade by increasing internode length, height, and specific leaf area, consistent with the shade avoidance response; paired shade species exhibited reduced plasticity overall, and only one trait (specific leaf area) responded to experimental shade. Conclusions: Our results suggest that milkweeds colonized shaded environments multiple times using a light-capture strategy distinct from the ancestral (putatively shade avoidant) strategy, including a general attenuation of plasticity in response to variable light conditions. This pattern bolsters the notion that shade avoidance and tolerance represent divergent evolutionary strategies for maximizing performance under qualitatively different types of shade.

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Photoreceptor Activity Contributes to Contrasting Responses to Shade in Cardamine and Arabidopsis Seedlings

Cited by 31 publications

References 66 publications

Progress of Research on the Regulatory Pathway of the Plant Shade-Avoidance Syndrome

Progress of Research on the Regulatory Pathway of the Plant Shade-Avoidance Syndrome

Adaptation to plant shade relies on rebalancing the transcriptional activity of the PIF-HFR1 regulatory module

Evolution of shade tolerance is associated with attenuation of shade avoidance and reduced phenotypic plasticity in North American milkweeds

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