Photoreceptor proteins and melatonin rhythm generating AANAT in the carp pineal: Temporal organization and correlation with natural photo-thermal cues

Seth, Mohua; Maitra, Saumen Kumar

doi:10.1016/j.jphotobiol.2010.01.010

Cited by 10 publications

(13 citation statements)

References 44 publications

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“…The pineal in fish, including carp (Seth & Maitra, 2010a), performs two major rhythmic functions: photoreception associated with abundance of specific photoreceptor proteins in the light phase and augmentation of melatonin during the dark phase in a 24-h LD cycle. We recently showed that light-induced abundance of photoreceptor proteins in the carp pineal is stimulated by the cholinergic (both nicotinic and muscarinic) system and inhibited by dopaminergic signals, but is independent of functions of adrenergic (α 1 , α 2 , and β 1 ) receptors (Seth & Maitra, 2010c).…”

Section: Resultsmentioning

confidence: 99%

“…Pineal AANAT band intensity in each sample was normalized by the intensity of β-actin (internal standard) and expressed as relative densitometric units (ratio of AANAT band intensity to β-actin) (Seth & Maitra, 2010a, 2010b. Mean ± SE values of the relative densitometric data of immunoblots for AANAT as well as the pineal titers of melatonin and calcium in different groups were analyzed by one-way analysis of variance (ANOVA), where F values indicated significance, and means were compared by a post hoc multiple range test, with p < .05 taken as threshold of statistical significance (Zar, 1974).…”

Section: Discussionmentioning

confidence: 99%

“…The isolated pineals were incubated in RPMI 1640 medium in a chamber at 20°C ± 1°C in the presence of 95% O 2 and 5% CO 2 . Our recent studies revealed that melatonin synthesis in the carp pineal reaches its diurnal peak 6 h after the onset of darkness in a 12L:12D cycle (Seth & Maitra, 2010a), but such peak does not occur in presence of light (Seth & Maitra, 2010b). Moreover, a schedule of 6-h incubation is considered ideal for the measurement of any change in the profile of fish pineal AANAT (Thibault et al, 1993).…”

Section: Pineal Organ Culture and Experimental Paradigmmentioning

confidence: 99%

“…This was followed by incubation with the respective secondary antibody-(i) goat antirabbit IgG conjugated with alkaline phosphatase for AANAT or (ii) goat antimouse IgG conjugated with alkaline phosphatase for β-actin-at a dilution of 1:500 for 2 h at room temperature (22°C). The antibody used in this study was the same as previously used (Seth & Maitra, 2010a, 2010b to identify AANAT in the carp pineal. Nonetheless, the specificity of AANAT antibody was determined by a preabsorption test.…”

Section: Western Blot Analysismentioning

confidence: 99%

“…Immunolabelling was done by BCIP/NBT following the manufacturer's instructions. Individual band intensity of the Western blots was quantified by densitometry analysis using Image J software (Seth & Maitra, 2010a, 2010b.…”

Section: Western Blot Analysismentioning

confidence: 99%

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Neural Regulation of Dark-Induced Abundance of ArylalkylamineN-Acetyltransferase (AANAT) and Melatonin in the Carp (Catla catla) Pineal: AnIn VitroStudy

Seth

Maitra

2011

Chronobiology International

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In all the vertebrates, synthesis of melatonin and its rhythm-generating enzyme arylalkylamine N-acetyltransferase (AANAT) reaches its peak in the pineal during the night in a daily light-dark cycle, but the role of different neuronal signals in their regulation were unknown for any fish. Hence, the authors used specific agonist and antagonists of receptors for different neuronal signals and regulators of intracellular calcium (Ca(2+)) and adenosine 3',5'-cyclic monophosphate (cAMP) in vitro to study their effects on the abundance of AANAT and titer of melatonin in the carp (Catla catla) pineal. Western blot analysis followed by quantitative analysis of respective immunoblot data for AANAT protein, radioimmunoassay of melatonin, and spectrophotometric analysis of Ca(2+) in the pineal revealed stimulatory effects of both adrenergic (α(1) and β(1)) and dopaminergic (D(1)) agonists and cholinergic (both nicotinic and muscarinic) antagonists, inhibition by both adrenergic and dopaminergic antagonists and cholinergic agonists, but independent of the influence of any agonists or antagonists of α(2)-adrenergic receptors. Band intensity of AANAT and concentration of melatonin in the pineal were also enhanced by the intracellular calcium-releasing agent, activators of both calcium channel and adenylate cyclase, and phophodiesterase inhibitor, but suppressed by inhibitor of calcium channel and adenylate cyclase as well as activator of phophodiesterase. Moreover, an inhibitory effect of light on the pineal AANAT and melatonin was blocked by both cAMP and proteasomal proteolysis inhibitor MG132. Collectively, these data suggest that dark-induced abundance of AANAT and melatonin synthesis in the carp pineal are a multineuronal function, in which both adrenergic (α(1) and β(1), but not α(2)) and dopaminergic signals are stimulatory, whereas cholinergic signals are inhibitory. This study also provides indications, though arguably not conclusive evidence, that in either case the neuronal mechanisms follow a signal-transduction pathway in which Ca(2+) and cAMP may act as the intracellular messengers. It also appears that proteasomal proteolysis is a conserved event in the regulation of AANAT activity in vertebrates.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Pineal Organ Culture and Experimental Paradigmmentioning

confidence: 99%

Section: Western Blot Analysismentioning

confidence: 99%

Section: Western Blot Analysismentioning

confidence: 99%

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Neural Regulation of Dark-Induced Abundance of ArylalkylamineN-Acetyltransferase (AANAT) and Melatonin in the Carp (Catla catla) Pineal: AnIn VitroStudy

Seth

Maitra

2011

Chronobiology International

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Gut melatonin response to microbial infection in carp Catla catla

Pal

Hasan

Maitra

2015

Fish Physiol Biochem

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The purpose of present study was to demonstrate the response of gut melatoninergic system to Aeromonas hydrophila infection for 3 or 6 days and search for its correlation with the activity of different antioxidative and digestive enzymes to focus their interplay under pathophysiological conditions in carp (Catla catla). Microscopic study of gut in infected fish revealed degenerative changes in the tunica mucosa and lamina propria layers with sloughed off epithelial cells in the lumen. The activity of each digestive enzyme was reduced, but the levels of melatonin, arylalkylamine-N-acetyl transferase protein, the key regulator of melatonin biosynthesis, and different enzymatic antioxidants in gut were gradually and significantly increased with the progress of infection. Gut melatonin concentrations in A. hydrophila challenged carp by showing a positive correlation with the activity of each antioxidative enzyme, and a negative correlation with different digestive enzymes argued in favor of their functional relation, at least, during pathological stress. Moreover, parallel changes in the gut and serum melatonin titers indicated possible contribution of gut to circulating melatonin. Collectively, present carp study provided the first data to suggest that endogenous gut melatonin may be implicated to the mechanism of response to microbial infections in any fish species.

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Melatonin concentrations in relation to oxidative status and oocyte dynamics in the ovary during different reproductive phases of an annual cycle in carp Catla catla

2014

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Photoreceptor proteins and melatonin rhythm generating AANAT in the carp pineal: Temporal organization and correlation with natural photo-thermal cues

Cited by 10 publications

References 44 publications

Neural Regulation of Dark-Induced Abundance of ArylalkylamineN-Acetyltransferase (AANAT) and Melatonin in the Carp (Catla catla) Pineal: AnIn VitroStudy

Neural Regulation of Dark-Induced Abundance of ArylalkylamineN-Acetyltransferase (AANAT) and Melatonin in the Carp (Catla catla) Pineal: AnIn VitroStudy

Gut melatonin response to microbial infection in carp Catla catla

Melatonin concentrations in relation to oxidative status and oocyte dynamics in the ovary during different reproductive phases of an annual cycle in carp Catla catla

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