Photoregulation of Protochlorophyllide Oxidoreductase and Rubisco Large Subunit Accumulation in Phytochrome A-Deficient Transgenic Tobacco Plants

Protochlorophyllide reduction - what is new in 2005?

“…Similar changes in the non-photoactive and photoactive Pchlide contents, but of lower amplitude, were also observed in pea leaves and tobacco cotyledons. These results agree with those obtained by Antipova et al (2004) (see above), but contrast with those obtained with upper stems of tomato and Arabidopsis cotyledons because in these species FR did not induce any change in the non-photoactive to photoactive Pchlide ratio. Sineshchekov et al (2004) concluded that the effect of FR on biogenesis of photoactive Pchlide is not only dependent on the species but also on the organ.…”

“…They showed that when grown in constant FR (λ≥720 nm), the content in non-photoactive and photoactive Pchlide in upper stems of tobacco and pea increased dramatically, while no Chl molecules accumulated. The presence or absence of Chl molecules in FR-irradiated tissues seems to depend primarily on the quality of the filters used for irradiation (see Antipova et al 2004). Similar changes in the non-photoactive and photoactive Pchlide contents, but of lower amplitude, were also observed in pea leaves and tobacco cotyledons.…”

“…When grown in far-red (FR) radiation (λ≥680 nm), the mutant seedlings contained more than three times more Chl molecules than WT, which was able to accumulate only a very small amount of Chl due to the irradiation conditions (see Sineshchekov et al 2004). Dot-blot analysis of LPOR showed that under FR, but not in darkness, the anti-sense mutant contained more LPOR than the WT (Antipova et al 2004) confirming that PhyA regulates the lpor gene expression. Sineshchekov et al (2004) studied another aspect of photoactive Pchlide formation, the relative accumulation of non-photoactive and photoactive Pchlide.…”

“…) Another target of PhyA seems to be the process leading to the formation of the photoactive Pchlide aggregates. Two studies dealt with this particular step of the biogenesis of the photosynthetic apparatus: Antipova et al (2004) investigated the control of LPOR accumulation in tobacco by PhyA comparing the LPOR amount in WT and phyA anti-sense transgenic mutant of tobacco. When grown in far-red (FR) radiation (λ≥680 nm), the mutant seedlings contained more than three times more Chl molecules than WT, which was able to accumulate only a very small amount of Chl due to the irradiation conditions (see Sineshchekov et al 2004).…”

Etioplast and etio-chloroplast formation under natural conditions: the dark side of chlorophyll biosynthesis in angiosperms

“…At the day-night transition phytochrome is inactivated and LPOR-A production and Pchlide accumulation are resumed, leading to the accumulation of photoactive Pchlide (Antipova et al 2004;Apel 1981;Ryberg and Terry 2002). The accumulation of photoactive ternary complexes of Pchlide and LPOR-A triggers the reformation of small PLBs that are connected to the developing thylakoids (e.g., Schoefs and Franck 2008).…”