Photosensitivity responses ofSagittula stellataprobed by FTIR, fluorescence and Raman microspectroscopy

Abstract

Raman, fluorescence and FTIR experiments of prestine Sagittula stellata and Sagittula stellata–metal ion complexes grown in light and in dark were performed to probe the photosensitivity response of the cellular components in the marine bacterium.

Bacterial Colonization on the Surface of Copper Sulfide Minerals Probed by Fourier Transform Infrared Micro-Spectroscopy

“…We suggest that the "direct-contact mechanism" shown in Figure 4 of bioleaching bacteria is the net result of miscellaneous interactions of the secondary structural motif. Raman and FTIR spectroscopies have been applied in our laboratory for more than 25 years for the investigation of biological mechanisms of NO and O2 respiration by heme-copper enzymes and the interactions of microorganisms with surfaces and metals [22][23][24][25][26][27][28][29][30][31][32][33][34][35]. The present work extends our previous investigation of the bioleaching dynamics of Cu-containing minerals, and provides analysis of the secondary structure of the interacting system between the protein band of extracellular polymeric substances and the surface of chalcopyrite.…”

Locally Enhanced Electric Field Treatment of E. coli: TEM, FT-IR and Raman Spectrometry Study

“…The band, originating from the CH 3 vibrations, at 1394 cm −1 shifts to 1388 cm −1 (Figure 6, Table 2). All the peaks at 2870-2890 cm −1 , 2933 cm −1 , 2960 cm −1 , 2975 cm −1 , assigned to CH 3 and CH 2 vibrations of fatty acids in the cell membrane, demonstrate the intensity decrease [59,60]. Moreover, the peak intensity ratio I 1100 /I 1123 provides the order/disorder extent of the conformation of the alkyl chain of lipids and represents the ratio of disorder/order conformations of the C-C bond vibration in the alkyl chains [61,62].…”

Application of double-pulse laser-induced breakdown spectroscopy (DP-LIBS), Fourier transform infrared micro-spectroscopy and Raman microscopy for the characterization of copper-sulfides

“…FTIR spectroscopy is an analytical spectroscopic method for studying subtle changes in secondary structure of proteins as it allows the analysis of chemical bonds. 3,4,47,[50][51][52][53] Monitoring the conformations changes of protein structures may originate from interactions with other biomaterials or inorganic matter. Such interactions are of high relevance in various physiological cellular processes such as cellular attachment in sulde mineral surfaces during bioleaching processing.…”