Photosensitizers in prostate cancer therapy

Gheewala, Taher; Skwor, Troy; Munirathinam, Gnanasekar

doi:10.18632/oncotarget.15496

Cited by 72 publications

(45 citation statements)

References 118 publications

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“…67 To evaluate the efficacy of PS-PhMV compared to free PS, we incubated the cells with each reagent for 8 h, with concentrations of PS ranging from 0.01–5.0 μ M. Treatment was induced by exposing the cells to white light, whereas controls were kept in the dark. Unloaded VLPs were used as additional controls, at the same protein concentration as the highest dose of drug-loaded particles.…”

Section: Resultsmentioning

confidence: 99%

Physalis Mottle Virus-Like Particles as Nanocarriers for Imaging Reagents and Drugs

et al. 2017

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Platform technologies based on plant virus nano-particles (VNPs) and virus-like particles (VLPs) are attracting the attention of researchers and clinicians because the particles are biocompatible, biodegradable, noninfectious in mammals, and can readily be chemically and genetically engineered to carry imaging agents and drugs. When the Physalis mottle virus (PhMV) coat protein is expressed in Escherichia coli, the resulting VLPs are nearly identical to the viruses formed in vivo. Here, we isolated PhMV-derived VLPs from ClearColi cells and carried out external and internal surface modification with fluorophores using reactive lysine-N-hydroxysuccinimide ester and cysteine-maleimide chemistries, respectively. The uptake of dye-labeled particles was tested in a range of cancer cells and monitored by confocal microscopy and flow cytometry. VLPs labeled internally on cysteine residues were taken up with high efficiency by several cancer cell lines and were colocalized with the endolysosomal marker LAMP-1 within 6 h, whereas VLPs labeled externally on lysine residues were taken up with lower efficiency, probably reflecting differences in surface charge and the propensity to bind to the cell surface. The infusion of dye and drug molecules into the cavity of the VLPs revealed that the photosensitizer (PS), Zn-EpPor, and the drugs crystal violet, mitoxantrone (MTX), and doxorubicin (DOX) associated stably with the carrier via noncovalent interactions. We confirmed the cytotoxicity of the PS-PhMV and DOX-PhMV particles against prostate cancer, ovarian and breast cancer cell lines, respectively. Our results show that PhMV-derived VLPs provide a new platform technology for the delivery of imaging agents and drugs, with preferential uptake into cancer cells. These particles could therefore be developed as multifunctional tools for cancer diagnosis and therapy.

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Section: Resultsmentioning

confidence: 99%

Physalis Mottle Virus-Like Particles as Nanocarriers for Imaging Reagents and Drugs

et al. 2017

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“…Conventional therapies may lead to recurrence and progression of disease, being necessary the development of new approaches. Among these, photodynamic therapy (PDT) is a promising strategy in delivering focal treatment in PCa . PDT involves the use of a photosensitizer, quite harmless in the dark, that generates cytotoxic reactive oxygen species (ROS) upon visible light irradiation.…”

Section: Introductionmentioning

confidence: 99%

4,6,4′‐trimethylangelicin shows high anti‐proliferative activity on DU145 cells under both UVA and blue light

et al. 2018

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“…Одна из наиболее сложных проблем -реабилитация онкологических больных после проведенного радикального лечения. В настоящее время среди возможных вариантов лечения локализованного рака предстательной железы все большее значение приобретают малоинвазивные методики локальной терапии, такие как ультразвуковая и радиочастотная аблация, брахитерапия, криотерапия и фотодинамическая терапия (ФДТ) [7][8][9]. В последние десятилетия ФДТ показала свою перспективность при лечении опухолей различных локализаций, в том числе РПЖ.…”

Section: Introductionunclassified

“…Фотодинамическая терапия может применяться как малоинвазивная терапия при лечении РПЖ с использованием различных ФС, находящихся на разных стадиях клинических испытаний: «Фоскан» (mTHPC) с длиной волны возбуждения 652 нм, TOOKAD (WST09 и WST11) -763 нм, MOTEXAFIN LUTETIUM (MLu) -732 нм, аминолевулиновая кислота (5-ALA) (как пролекарство -предшествен-ник протопорфирина IX) и «Фотофрин» -630 нм, Визудин (Вертепорфин) -689 нм, производные фталоцианина -670-690 нм [9,13,14].…”

Section: Introductionunclassified

“…Использование бактериохлоринов, имеющих интенсивное поглощение в интервале 720-800 нм, позволяет увеличить эффективность ФДТ за счет более глубокого проникновения излучения в опухоль. Однако многие из них имеют ограниченное применение из-за низкой химической стабильности, фотостабильности и высокой гидрофобности, что требует поиска транспортных систем, эффективно доставляющих ФС к опухолевой ткани [9,13]. Целью данного исследования стало изучение специфической фотоиндуцированной активности нового препарата «Бактериосенс» (на основе синтетического производного бактериохлорина) в системе in vitro и in vivo c использованием опухолевых моделей различного гистогенеза и РПЖ.…”

Section: Introductionunclassified

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Preclinical trial of Bacteriosens used for the photodynamic therapyof malignant tumors, including prostate cancer

Morozova

Plotnikova

Плютинская

et al. 2018

Rossijskij bioterapevtičeskij žurnal

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Introduction.Bacteriochlorins are the most promising photosensitizers absorbing in the near-infrared spectral region. Their use can enhance the efficiency of photodynamic therapy due to the deeper penetration of radiation into the tumor.Objectiveto conduct a preclinical study of the photoinduced antitumor activity and biodistribution of Bacteriosens.Materials and methods.Bacteriosens is a preparation based on meso-tetra(3-pyridyl)bacteriochlorin absorbing at 747 nm. Photoinduced cytotoxicity was investigated in vitro using human tumor cells: A549, Hep 2, BT-474, MCF-7, SK-BR-3, PC3, and EJ and murine tumor cells: S37, C26, and LLC. In vivo studies were performed in mice with large and small tumors (S37, LLC, and C26).Results.In vitro investigation show that bacteriosens during optical irradiation led to the effective suppression of tumor cell growth in culture (the IC50 value varied from 0,08μМ to 1,21 μМ) and had no toxicity without exposure to light. The effective photodynamic therapy regimen using Bacteriosens in mice with inoculated small and large tumors of different genesis resulted in regression of a primary tumor node on 90–100 % of the animals in the absence of tumor recurrence within 90 days after treatment.Conclusion.Bacteriosens is a promising agent for the photodynamic therapy of small and large tumors; it can be successfully used as an alternative, organ-sparing minimally invasive treatment for malignant tumors, including prostate cancer.

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Photosensitizers in prostate cancer therapy

Cited by 72 publications

References 118 publications

Physalis Mottle Virus-Like Particles as Nanocarriers for Imaging Reagents and Drugs

Physalis Mottle Virus-Like Particles as Nanocarriers for Imaging Reagents and Drugs

4,6,4′‐trimethylangelicin shows high anti‐proliferative activity on DU145 cells under both UVA and blue light

Preclinical trial of Bacteriosens used for the photodynamic therapyof malignant tumors, including prostate cancer

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