Photosensitizing Activity of Carcinogenic and Noncarcinogenic Polycyclic Hydrocarbons on Cultured Cells

Takayama, Susumu; Ojima, Yoshio

doi:10.1266/jjg.44.231

Cited by 1 publication

(1 citation statement)

References 26 publications

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“…Morphological changes in lysosomes and mitochondria often coincided with changes in membrane potential, pH gradient or membrane permeability. − Under some circumstances such changes resulted in the release of a resident, organelle-specific enzyme into the cytosol or into the extracellular compartment. Thus the detection of fluctuation in voltage or pH gradients, or the detection of released organelle components, was used as evidence for accumulation of exogenous small molecules in specific organelles, from the 1970s − and continuing to this day. − …”

Section: Pharmacological Effects As Evidence For Specific Organelle A...mentioning

confidence: 99%

The Subcellular Distribution of Small Molecules: From Pharmacokinetics to Synthetic Biology

et al. 2011

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The systemic pharmacokinetics and pharmacodynamics of small molecules are determined by subcellular transport phenomena. Although approaches used to study the subcellular distribution of small molecules have gradually evolved over the past several decades, experimental analysis and prediction of cellular pharmacokinetics remains a challenge. In this article, we surveyed the progress of subcellular distribution research since the 1960s, with a focus on the advantages, disadvantages and limitations of the various experimental techniques. Critical review of the existing body of knowledge pointed to many opportunities to advance the rational design of organelle-targeted chemical agents. These opportunities include: 1) development of quantitative, nonfluorescence-based, whole cell methods and techniques to measure the subcellular distribution of chemical agents in multiple compartments; 2) exploratory experimentation with nonspecific transport probes that have not been enriched with putative, organelle-targeting features; 3) elaboration of hypothesis-driven, mechanistic and modeling-based approaches to guide experiments aimed at elucidating subcellular distribution and transport; and 4) introduction of revolutionary conceptual approaches borrowed from the field of synthetic biology combined with cutting edge experimental strategies. In our laboratory, state-of-the-art subcellular transport studies are now being aimed at understanding the formation of new intracellular membrane structures in response to drug therapy, exploring the function of drug-membrane complexes as intracellular drug depots, and synthesizing new organelles with extraordinary physical and chemical properties.

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Section: Pharmacological Effects As Evidence For Specific Organelle A...mentioning

confidence: 99%

The Subcellular Distribution of Small Molecules: From Pharmacokinetics to Synthetic Biology

et al. 2011

View full text Add to dashboard Cite

show abstract

Photosensitizing Activity of Carcinogenic and Noncarcinogenic Polycyclic Hydrocarbons on Cultured Cells

Cited by 1 publication

References 26 publications

The Subcellular Distribution of Small Molecules: From Pharmacokinetics to Synthetic Biology

The Subcellular Distribution of Small Molecules: From Pharmacokinetics to Synthetic Biology

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