Photoswitchable Enzymatic Biofuel Cell Based on Fusion Protein with Natural Photoreceptor Vivid

Yang, Chennan; Li, Zehua; Ma, Chunling; Zhu, Zhiguang

doi:10.1021/acsabm.1c01268

Cited by 3 publications

(1 citation statement)

References 29 publications

(57 reference statements)

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“…In nature, these systems are ubiquitous and often categorized as photoreceptor proteins (PRP), which are utilized by many organisms, from bacteria to mammals [10,11]. PRPs have inspired a variety of non-native uses in fields as diverse as neuroscience [4], targeted theranostics [2], fundamental biophysics [1,12], and fuel cells [13].…”

Section: Introductionmentioning

confidence: 99%

Irradiation of ZnPPIX Complexed with Bovine β-Lactoglobulin Causes Chemical Modifications and Conformational Changes of the Protein

Albalawi,

Castillo,

Denton

et al. 2023

Physchem

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Photosensitization of proteins mediated by chromophores is a mechanism commonly employed by nature and mimicked in a broad array of laboratory research and applications. Nature has evolved specialized complexes of proteins and photosensitizers (PS) that assemble to form photoreceptor proteins (PRP). These are used by many organisms in diverse processes, such as energy conversion, protection against photodamage, etc. The same concept has been used in laboratory settings for many applications, such as the stimulation of neurons or the selective depletion of proteins in a signaling pathway. A key issue in laboratory settings has been the relationship between the photooxidation of proteins and conformational changes in host proteins. For several years, we have been interested in creating non-native PRP using porphyrin PS. In this study, we investigated the self-assembled complex between zinc protoporphyrin IX (ZnPPIX) and bovine β-lactoglobulin (BLG) as a model of non-native PRP. Since BLG undergoes a significant conformational transition near physiological pH, the study was carried out at acidic (pH 5) and alkaline (pH 9) conditions where the two conformations are respectively prevalent. We employed a series of steady-state and time-resolved optical spectroscopies as well as gel electrophoresis to experimentally characterize the photosensitization mechanisms and their effect on the host protein. Our results show that ZnPPIX prompts light-dependent modifications of BLG, which appear to be much more significant at alkaline pH. The modifications seem to be driven by photooxidation of amino acid residues that do not lead to the formation of cross-links or protein fragmentation.

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Section: Introductionmentioning

confidence: 99%

Irradiation of ZnPPIX Complexed with Bovine β-Lactoglobulin Causes Chemical Modifications and Conformational Changes of the Protein

Albalawi,

Castillo,

Denton

et al. 2023

Physchem

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Oxygen reduction electrocatalysis: From conventional to single-atomic platinum-based catalysts for proton exchange membrane fuel cells

Yuan,

Zhang,

Zhang

2023

Front. Energy

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Cooperative Control of Bioelectrocatalytic Activity for Thermo- and Photo-Switchable Cup-Stacked Carbon Nanofiber Electrodes Modified with Phase Transition Polymer and Heme Peptide

Tani,

Tanigawa,

Liu

et al. 2024

Langmuir

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Empowering biocatalyst-modified electrodes with the ability to both enforce and perceive will enable the development of intrinsically switchable bioelectrode systems, which exhibit autonomous and heteronomous actions specific to living organisms. However, the electrocatalytic activity of switchable bioelectrodes reported so far has been controlled by changes in the rate of substrate transport to biocatalysts. Here, we prepared a cup-stacked carbon nanofiber (CSCNF) electrode modified with a thermoresponsive N-isopropylacrylamide-based polymer containing peroxidase model compounds (HP). As CSCNFs worked as a converter from near-infrared (NIR) light to heat, bioelectrocatalytic activity of the electrode to H2O2 reduction was reversibly controlled by changes in the amount of electroactive HP, based on expanded and contracted states of the polymers induced by not only environmental temperature changes but also external NIR light irradiation. This intrinsically switchable bioelectrode technique would hold promise for adding new performances in electrochemical biosensors and biofuel cells, for example, autonomous and heteronomous tunable sensitivity and capacity.

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Photoswitchable Enzymatic Biofuel Cell Based on Fusion Protein with Natural Photoreceptor Vivid

Cited by 3 publications

References 29 publications

Irradiation of ZnPPIX Complexed with Bovine β-Lactoglobulin Causes Chemical Modifications and Conformational Changes of the Protein

Irradiation of ZnPPIX Complexed with Bovine β-Lactoglobulin Causes Chemical Modifications and Conformational Changes of the Protein

Oxygen reduction electrocatalysis: From conventional to single-atomic platinum-based catalysts for proton exchange membrane fuel cells

Cooperative Control of Bioelectrocatalytic Activity for Thermo- and Photo-Switchable Cup-Stacked Carbon Nanofiber Electrodes Modified with Phase Transition Polymer and Heme Peptide

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