Photoswitchable Nanoprobes for Biological Imaging Applications

Zhang, Tian; Wu, Wuwei; Li, Alexander D. Q.

doi:10.1002/9783527632015.ch1

Cited by 4 publications

(7 citation statements)

References 114 publications

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“…7,27,29,[75][76][77][78][79] The bottleneck is that multiple emitting fluorophores located within the diffraction-limited area cannot be resolved simultaneously. However, the diffraction limit originating from the wave properties of light restricts the optical microscope resolution to ∼300 nm, a level two of orders of magnitude coarser than nanoscale molecules; thus many intracellular organelles and their associated molecular structures remained unresolvable.…”

Section: Photoswitchable Fluorescent Nps Enable High-resolution Imagingmentioning

confidence: 99%

“…6,[23][24][25] In this review, we outline the major classes of SP/DAE-based photoswitchable fluorescent NPs developed in recent years while photoswitchable single-molecule fluorophores and fluorescent proteins, as well as NPs constructed from other types of photoswitches are beyond the scope of this review. Additionally, considering that SP/DAE-based dynamic materials have been reviewed extensively, 6,7,[26][27][28][29] we emphasize on the emerging applications of photoswitchable fluorescent NPs in biological fluorescence assays and detection with a few representative paradigms.…”

Section: Jie Wangmentioning

confidence: 99%

“…The controllable fluorescence on-off photoswitching plays a critical role in these revolutionary ultrahigh-resolution imaging techniques. 7,27,29,[75][76][77][78][79] The bottleneck is that multiple emitting fluorophores located within the diffraction-limited area cannot be resolved simultaneously. However, if only one fluorophore is emitted at a given time, then its location can be determined down to nanometer accuracy, far beyond the Abbe diffraction limit.…”

Section: Photoswitchable Fluorescent Nps Enable High-resolution Imagingmentioning

confidence: 99%

“…[1][2][3][4][5][6] Among different forms of stimulus-induced signal modulation, fluorescence is particularly promising because fluorescence offers not only ultrahigh sensitivity but also selfidentifying characteristics such as emission maximum, emission color, and signal lifetime. 7 Light, as an external stimulus that can modulate the selected property of a dynamic material, can be manipulated easily and delivered with precise spatiotemporal control. Moreover, it requires no physical contact thus avoiding potential risk of chemical contamination.…”

Section: Introductionmentioning

confidence: 99%

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Photoswitchable fluorescent nanoparticles and their emerging applications

Zhang

Wang

et al. 2015

Nanoscale

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Although fluorescence offers ultrasensitivity, real-world applications of fluorescence techniques encounter many practical problems. As a noninvasive means to investigate biomolecular mechanisms, pathways, and regulations in living cells, the intrinsic heterogeneity and inherent complexity of biological samples always generates optical interferences such as autofluorescence. Therefore, innovative fluorescence technologies are needed to enhance measurement reliability while not compromising sensitivity. In this review, we present current strategies that use photoswitchable nanoparticles to address these real-world challenges. The unique feature in these photoswitchable nanoparticles is that fundamental molecular photoswitches are playing the critical role of fluorescence modulation rather than traditional methods like modulating the light source. As a result, new innovative technologies that have recently emerged include super-resolution imaging, frequency-domain imaging, antiphase dual-color correlation, etc. Some of these methods improve imaging resolution down to the nanometer level, while others boost the detection sensitivity by orders of magnitude and confirm the nanoparticle probes unambiguously. These enhancements, which are not possible with non-photoswitching molecular probes, are the central topics of this review.

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Section: Photoswitchable Fluorescent Nps Enable High-resolution Imagingmentioning

confidence: 99%

Section: Jie Wangmentioning

confidence: 99%

Section: Photoswitchable Fluorescent Nps Enable High-resolution Imagingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Photoswitchable fluorescent nanoparticles and their emerging applications

Zhang

Wang

et al. 2015

Nanoscale

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“…Since the resolution can be measured based on the switching of the fluorescent probes between two distinctively different fluorescent states, either fluorescent “ON” and “OFF” states or two fluorescent states with distinct color. Furthermore, the fluorescent probes must be actively varied (usually via photoswitching or photoactivation) in time to ensure that only an optically resolvable subset of fluorophores is activated at any time in a diffraction—limited region, thereby allowing their localization with high accuracy [ 29 ]. For example, Santra’s group described a method for synthesis of dopamine dithiocarbamate (DDTC) capped with CdS:Mn/ZnS core/shell QDs using a water-in-oil (W/O) microemulson mechanism [ 30 ].…”

Section: Semiconductor Qds-based Fluorescence Approaches For Biomomentioning

confidence: 99%

Semiconductor Nanomaterials-Based Fluorescence Spectroscopic and Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometric Approaches to Proteome Analysis

Kailasa

Cheng

2013

Materials

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Semiconductor quantum dots (QDs) or nanoparticles (NPs) exhibit very unusual physico-chemcial and optical properties. This review article introduces the applications of semiconductor nanomaterials (NMs) in fluorescence spectroscopy and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) for biomolecule analysis. Due to their unique physico-chemical and optical properties, semiconductors NMs have created many new platforms for investigating biomolecular structures and information in modern biology. These semiconductor NMs served as effective fluorescent probes for sensing proteins and cells and acted as affinity or concentrating probes for enriching peptides, proteins and bacteria proteins prior to MALDI-MS analysis.

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Photoswitching-Enabled Novel Optical Imaging: Innovative Solutions for Real-World Challenges in Fluorescence Detections

2012

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Because of its ultrasensitivity, fluorescence offers a noninvasive means to investigate biomolecular mechanisms, pathways, and regulations in living cells, tissues, and animals. However, real-world applications of fluorescence technologies encounter many practical challenges. For example, the intrinsic heterogeneity of biological samples always generates optical interferences. High background such as autofluorescence can often obscure the desired signals. Finally, the wave properties of light limit the spatial resolution of optical microscopy. The key to solving these problems involves using chemical structures that can modulate the fluorescence output. Photoswitchable fluorescent molecules that alternate their emissions between two colors or between bright-and-dark states in response to external light stimulation form the core of these technologies. For example, molecular fluorescence modulation can switch fluorophores on and off. This feature supports super-resolution, which enhances resolution by an order of magnitude greater than the longstanding diffraction-limit barrier. The reversible modulation of such probes at a particular frequency significantly amplifies the frequency-bearing target signal while suppressing interferences and autofluorescence. In this Account, we outline the fundamental connection between constant excitation and oscillating fluorescence. To create molecules that will convert a constant excitation into oscillating emission, we have synthesized photoswitchable probes and demonstrated them as proofs of concept in super-resolution imaging and frequency-domain imaging. First, we introduce the design of molecules that can convert constant excitation into oscillating emission, the key step in fluorescence modulation. Then we discuss various technologies that use fluorescence modulation: super-resolution imaging, dual-color imaging, phase-sensitive lock-in detection, and frequency-domain imaging. Finally, we present two biological applications to demonstrate the power of photoswitching-enabled fluorescence imaging. Because synthetic photoswitchable probes can be much smaller, more versatile, and more efficient at high-performance modulation experiments, they provide a complement to photoswitchable fluorescent proteins. Although new challenges remain, we foresee a bright future for photoswitching-enabled imaging and detection.

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Photoswitchable Nanoprobes for Biological Imaging Applications

Cited by 4 publications

References 114 publications

Photoswitchable fluorescent nanoparticles and their emerging applications

Photoswitchable fluorescent nanoparticles and their emerging applications

Semiconductor Nanomaterials-Based Fluorescence Spectroscopic and Matrix-Assisted Laser Desorption/Ionization (MALDI) Mass Spectrometric Approaches to Proteome Analysis

Photoswitching-Enabled Novel Optical Imaging: Innovative Solutions for Real-World Challenges in Fluorescence Detections

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