Photoswitching of Site-Selective RNA Scission by Sequential Incorporation of Azobenzene and Acridine Residues in a DNA Oligomer

Kuzuya, Akinori; Tanaka, Keita; Komiyama, Makoto

doi:10.4061/2011/162452

Cited by 6 publications

(5 citation statements)

References 23 publications

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“…Two interesting new approaches to reversibly photoregulate RNA cleavage have been published. Komiyama, Kuzuya, and Tanaka demonstrated the regulation of acridine‐assisted RNA scission through Lu 3+ ions by incorporating an acridine and an adjacent azobenzene residue in a DNA counterstrand 333. Site‐selective RNA activation by acridine was reduced through stacking interactions when the azobenzene residue was in the trans state, whereas switching to the cis isomer stopped the acridine from stacking.…”

Section: Reversible Photoswitchingmentioning

confidence: 99%

Light‐Controlled Tools

et al. 2012

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Spatial and temporal control over chemical and biological processes plays a key role in life, where the whole is often much more than the sum of its parts. Quite trivially, the molecules of a cell do not form a living system if they are only arranged in a random fashion. If we want to understand these relationships and especially the problems arising from malfunction, tools are necessary that allow us to design sophisticated experiments that address these questions. Highly valuable in this respect are external triggers that enable us to precisely determine where, when, and to what extent a process is started or stopped. Light is an ideal external trigger: It is highly selective and if applied correctly also harmless. It can be generated and manipulated with well-established techniques, and many ways exist to apply light to living systems--from cells to higher organisms. This Review will focus on developments over the last six years and includes discussions on the underlying technologies as well as their applications.

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Section: Reversible Photoswitchingmentioning

confidence: 99%

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et al. 2012

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“…By introducing azobenzene into a modified DNA, photoregulation of RNA cleavage by Lu III ion aided by an intercalator (e.g., acridine residue) was also realized. [65] The transazobenzene impeded the interaction of acridine so that RNA cleavage was slowed down, and isomerization to the cis form decreased the retarding effect because cis-azobenzene could flip out more easily. Azobenzene was also introduced into siRNA to suppress the off-target effect of RNAi.…”

Section: Other Applications Of Azobenzene-modified Dnamentioning

confidence: 99%

Modification of Nucleic Acids by Azobenzene Derivatives and Their Applications in Biotechnology and Nanotechnology

Li¹,

Wang²,

Liang³

2014

Chemistry An Asian Journal

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Azobenzene has been widely used as a photoregulator due to its reversible photoisomerization, large structural change between E and Z isomers, high photoisomerization yield, and high chemical stability. On the other hand, some azobenzene derivatives can be used as universal quenchers for many fluorophores. Nucleic acid is a good candidate to be modified because it is not only the template of gene expression but also widely used for building well-organized nanostructures and nanodevices. Because the size and polarity distribution of the azobenzene molecule is similar to a nucleobase pair, the introduction of azobenzene into nucleic acids has been shown to be an ingenious molecular design for constructing light-switching biosystems or light-driven nanomachines. Here we review recent advances in azobenzene-modified nucleic acids and their applications for artificial regulation of gene expression and enzymatic reactions, construction of photoresponsive nanostructures and nanodevices, molecular beacons, as well as obtaining structural information using the introduced azobenzene as an internal probe. In particular, nucleic acids bearing multiple azobenzenes can be used as a novel artificial nanomaterial with merits of high sequence specificity, regular duplex structure, and high photoregulation efficiency. The combination of functional groups with biomolecules may further advance the development of chemical biotechnology and biomolecular engineering.

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“…在此基础上, 谭及同事 [69] [70] . 类似地, 偶氮苯修饰的寡核苷酸还可以实现光调控核糖核酸酶H (RNase H)降解靶向RNA [21,71] . 将偶氮苯引入RNA链或脱氧核糖核酶(DNAzyme)不同位置, 可改变酶与底物复合物的拓扑结构, 从而隐藏切割位点或改变RNA底物与酶的结合能力, 也可以实现对RNA降解的光调控 [25,72] .…”

Section: 应用中存在的问题是在低温时异构化效率很低例如偶氮苯修饰的互补双链在37℃时紫外光照后仅得到unclassified

Reversible photoregulation in biological systems via introduction of azobenzene into nucleic acid

Kong

et al. 2018

Sci. Sin.-Chim

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Photoswitching of Site-Selective RNA Scission by Sequential Incorporation of Azobenzene and Acridine Residues in a DNA Oligomer

Cited by 6 publications

References 23 publications

Light‐Controlled Tools

Light‐Controlled Tools

Modification of Nucleic Acids by Azobenzene Derivatives and Their Applications in Biotechnology and Nanotechnology

Reversible photoregulation in biological systems via introduction of azobenzene into nucleic acid

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