Photosynthesis-deficient Mutants of <i>Chlamydomonas reinhardii</i> with Associated Light-sensitive Phenotypes

Spreitzer, Robert J.; Mets, Laurens

doi:10.1104/pp.67.3.565

Cited by 161 publications

(199 citation statements)

References 25 publications

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“…The ca-1-12-lC mutant described in this paper was recovered from a collection of acetate-requiring mutants based on its ability to grow photoautrophically at 5% CO2. This mendelian mutant was previously designated 12-IC and was found to have wild type fluorescence induction kinetics, PS-II activity, and Chl content (21). In addition, the level of RuBP carboxylase activity was found to be normal (data not shown).…”

Section: Resultsmentioning

confidence: 88%

“…Chlamydomonas reinhardii wild type strain 2137 mt+ and a collection of lightsensitive, acetate-requiring mutants described previously were maintained on acetate medium in the dark (21). For (21), these mutants were screened for ability to grow photoautotrophically in air enriched to 5% CO2.…”

Section: Methodsmentioning

confidence: 99%

“…Genetic analysis was performed with the centromere-marker strain pf-2 (paralyzed flagella) mr. Gamete induction, zygote maturation, zygote germination, and tetrad analysis were performed as described previously (21). Tetrads were scored as PD, NPD, or T following replica-plating at 80 ME m 2 s-' on minimal medium, minimal medium and 5% C02, and acetate medium, and on acetate medium in the dark.…”

Section: Methodsmentioning

confidence: 99%

“…Tetrads were scored as PD, NPD, or T following replica-plating at 80 ME m 2 s-' on minimal medium, minimal medium and 5% C02, and acetate medium, and on acetate medium in the dark. This information was used to calculate gene-centromere distance (21 Inorganic Carbon Accumulation. Uptake and accumulation of inorganic carbon and simultaneous carbon fixation were determined using silicone oil filtering centrifugation (2,10).…”

Section: Methodsmentioning

confidence: 99%

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Carbonic Anhydrase-Deficient Mutant of Chlamydomonas reinhardii Requires Elevated Carbon Dioxide Concentration for Photoautotrophic Growth

Spalding¹,

Spreitzer²,

Ogren³

1983

Plant Physiol.

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162

164

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A mendelian mutant of the unicellular green alp Chlamydomonas reinhardii has been isolated which is deficient in carbonic anhydrase (EC 4.2.1.1) activity. This mutant strain, designated ca-1-12-1C (gene locus ca-i), was selected on the basis of a high C02 requirement for photoautotrophic growth. Photosynthesis by the mutant at atmospheric C02 concentration was very much reduced compared to wild type and, unlike wild type, was strongly inhibited by 02. In contrast to a CO2 compensation concentration of near zero in wild type at all 02 concentrations examined, the mutant exhibited a high, 02-stimulated C02 compensation concentration. Evidence of photorespiratory activity in the mutant but not in wild type was obtained from the analysis of photosynthetic products in the presence of '4CO2. At

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Section: Resultsmentioning

confidence: 88%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Carbonic Anhydrase-Deficient Mutant of Chlamydomonas reinhardii Requires Elevated Carbon Dioxide Concentration for Photoautotrophic Growth

Spalding¹,

Spreitzer²,

Ogren³

1983

Plant Physiol.

Self Cite

162

164

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“…Chlamydomonas reinhardtii strain 21 37A+ was maintained on an acetate medium which was described previously (28 …”

Section: Materials and Methods Strain And Culture Conditionsmentioning

confidence: 99%

Chlamydomonas reinhardtii Phosphoribulokinase

Roesler¹,

Ogren²

1990

Plant Physiol.

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The sequence and kinetic properties of phosphoribulokinase purified from Chiamydomonas reinhardtii were determined and compared with the spinach (Spinacea oleracea) enzyme. Chiamydomonas phosphoribulokinase was purified to apparent homogeneity, with a specific activity of 410 micromoles per minute per milligram. Polyclonal antibodies to the purified protein were used to isolate a Chiamydomonas cDNA clone, which, upon sequencing, was found to contain the entire coding region. (6,11,21), and its location is consistent with results of affinity labeling of the enzyme with an ATP analog (1 1). Also, the roles of several metabolic effectors in regulation of spinach phosphoribulokinase have been determined (5). Investigations of phosphoribulokinase of several prokaryotic organisms have shown that the bacterial enzyme differs markedly from the spinach enzyme with respect to kinetic parameters, subunit size and number, pH optima, activation by thiols, and regulation by metabolites (1,15,26,29).In contrast to the knowledge of the higher plant and bacterial enzymes, little biochemical characterization and no sequence data have been reported for algal phosphoribulokinase. To gain insight into structure-function relationships of this enzyme, the sequence and kinetic parameters of phosphoribulokinase from the green alga Chlamydomonas reinhardtii were determined and compared with corresponding information for the spinach enzyme. MATERIALS AND METHODS Strain and Culture ConditionsChlamydomonas reinhardtii strain 21 37A+ was maintained on an acetate medium which was described previously (28

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Atrazine tolerance of algae isolated from two agricultural streams

Hersh

Crumpton

1989

Enviro Toxic and Chemistry

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Based on photosynthesis (oxygen evolution) EC50s determined from short (5 min) assays, green algae (Chlorella sp. and other species of Chlorococcales) isolated at random from an atrazine‒contaminated stream (Big Spring) in midsummer exhibited similar tolerances to atrazine (range of EC50s: 42–125 μg/L) compared to algae isolated at random from a noncontaminated stream (Osage Spring) (range of EC50s: 35–162 μg/L). A laboratory strain of Chlamydomonas reinhardii known to be susceptible to atrazine responded similarly (EC50: 45 μg/L). The EC50s of these algae are similar to those previously reported for laboratory strains. In contrast, an alga (Franceia sp., Chlorococcales) isolated at random during late spring from Big Spring responded similarly to a laboratory strain of Chlamydomonas reinhardii that is resistant to atrazine (Franceia sp. EC50s: 430–774 μg/L, C. reinhardii EC50: 466 μg/L). The EC50 values of these algae are near the high end of the range of EC50s reported for green algae.

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Photosynthesis-deficient Mutants of Chlamydomonas reinhardii with Associated Light-sensitive Phenotypes

Cited by 161 publications

References 25 publications

Carbonic Anhydrase-Deficient Mutant of Chlamydomonas reinhardii Requires Elevated Carbon Dioxide Concentration for Photoautotrophic Growth

Carbonic Anhydrase-Deficient Mutant of Chlamydomonas reinhardii Requires Elevated Carbon Dioxide Concentration for Photoautotrophic Growth

Chlamydomonas reinhardtii Phosphoribulokinase

Atrazine tolerance of algae isolated from two agricultural streams

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