Photosynthesis parameters during acclimatization of in vitro-grown olive plantlets

Chaari-Rkhis, A.; Maâlej, M.; Chelli-Chaabouni, Azza; Fki, Lotfi; Drira, Noureddine

doi:10.1007/s11099-015-0138-x

Cited by 11 publications

(4 citation statements)

References 26 publications

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“…[1][2][3] It is widely used extensively in agriculture to improve crops quality and yield for some fruits and vegetables. [4][5][6] It promotes cell division, stem elongation, seed germination, breaking dormancy, floral development, fruit set, shoot growth, and mobilization of food. [7][8][9] Gibberellic acid (GA) is bioactive and persistent in the soil for months.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and effect of a new Terbium gibberellic complex on the histopathological alteration induced by Gibberellic acid on liver and kidney of mice Mus musculus

Seleem

Hussein

2018

Chem Biol Drug Des

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The objective of this study was to synthesize Gibberellic lanthanide complex and evaluate its biological activity to reduce the Gibberellic acid toxicity on liver and kidney. The new bis(Gibberellic)-nitro-terbium(III) complex was characterized by different analytical methods: elemental analyses, UV-Vis, molar ratio, fluorescence, FT-IR, and TGA-DTA measurements. Thirty newborns were classified into three groups control, Gibberellic acid, and Terbium gibberellic acid complex. Livers and kidneys of studied groups proceed for general histology and immunohistochemical staining of Cyr61, cytochrome C, and TNFR2. From the absorption titration measurements, the binding constants of DNA with Tb(III)-(GA) complex and free ligand were found to be 3.9 × 10 and 2.1 × 10 m , respectively, with the stoichiometry of 1:1. Hypochromism was observed from the absorption titration experiment which indicates the intercalation of Tb(III)-(GA) complex between the base pairs of DNA. Gibberellic acid-treated group showed alteration in the histological picture of livers and kidneys that accompanied with the reduction in the expression of Cyr61, cyt C, and TNFR2. The amelioration was observed in Gibberellic acid complex with Terbium group. The study concluded that Terbium gibberellic complex is less dangerous effects than Gibberellic acid alone.

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Section: Introductionmentioning

confidence: 99%

Synthesis and effect of a new Terbium gibberellic complex on the histopathological alteration induced by Gibberellic acid on liver and kidney of mice Mus musculus

Seleem

Hussein

2018

Chem Biol Drug Des

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“…Moreover, during the acclimatization process the plantlets have poor photosynthetic capability and the leaves act as a source of carbohydrates for the newly developing leaves. This poor photosynthetic capability could cause the deaths of some of the micropropagated plantlets (Chaari-Rkhis et al 2015). Several studies have shown that the usage of 100% jiffy-7 was the suitable potting medium for the plantlets acclimatization (Yahya et al 2015;Nabilah et al 2017).…”

Section: Discussionmentioning

confidence: 99%

Direct Shoot Organogenesis and Clonal Fidelity Confirmation of Tongkat Ali (Eurycoma longifolia) using Molecular Markers

Alttaher¹

2021

IJAB

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Eurycoma longifolia is a medicinally potent plant found in the tropical forest of South-East Asia. Every part of the plant, especially the root is traditionally used as an aphrodisiac, anticancer and anti-inflammatory. E. longifolia is conventionally propagated by seeds but with low germination rate and efficiency. This has made an in vitro propagation of E. longifolia a desirable alternative. Hence, this study reports an effective method of direct organogenesis of shoot. In vitro seedling’s leaves were cultured on Murashige and Skoog (MS) medium containing 1.0 mg L-1 6-benzylaminopurine (BAP), producing 1.8 ± 0.5 shoots per leaf with a regeneration frequency of 68.2%. The shoot buds were directly formed from leaves without intermediate callus formation. To obtain complete plantlets, the shoots were in vitro rooted with an average number of 4.2 ± 0.4 roots per shoot in half-strength MS (½MS) medium supplemented with 0.5 mg L-1 indole-3-butyric acid (IBA). Regenerated plantlets were successfully acclimatized to field conditions with an 85% survival rate. Genetic fidelity of the micropropagated plantlets was evaluated using Simple Sequence Repeat (SSR) and Inter Simple Sequence Repeat (ISSR) analysis. The results showed that the monomorphic banding patterns of in vitro raised plantlets and their mother plant were similar, confirming its homogeneity and the reliability of the multiplication system. © 2021 Friends Science Publishers

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“…Light availability is an important factor for a successful process of acclimatisation, involving a proper activation of the photosynthesis process [2]. The transition from in vitro to ex vitro conditions has been achieved in numerous plant species, both herbaceous and woody plants [2,4,8,10,[17][18][19][20].…”

Section: Discussionmentioning

confidence: 99%

“…These authors found that acclimatised plants to ex vitro conditions increased the photosynthesis rate, which correlated with higher chlorophyll contents as well as Fv/Fm and Y(II) fluorescence parameters. In other studies, the authors used the photosynthesis rate or the chlorophyll a fluorescence technique, as well as the measurement of the antioxidant defences as indicators of the evolution of the acclimatisation process [1][2][3][4]17,19]. Accordingly, the monitoring of some physiological, biochemical, and molecular parameters can be useful tools to determine the success of ex vitro acclimatisation of in vitro plantlets.…”

Section: Chlorophyll Fluorescencementioning

confidence: 99%

Antioxidant Metabolism and Chlorophyll Fluorescence during the Acclimatisation to Ex Vitro Conditions of Micropropagated Stevia rebaudiana Bertoni Plants

et al. 2019

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In this study, the functioning of antioxidant metabolism and photosynthesis efficiency during the acclimatisation of Stevia rebaudiana plants to ex vitro conditions was determined. A high percentage of acclimatised plants (93.3%) was obtained after four weeks. According to the extent of lipid peroxidation, an oxidative stress occurred during the first hours of acclimatisation. A lower activity of monodehydroascorbate reductase (MDHAR) than dehydroascorbate reductase (DHAR) was observed after 2 days of acclimatisation. However, after 7 days of acclimatisation, stevia plants activated the MDHAR route to recycle ascorbate, which is much more efficient energetically than the DHAR route. Superoxide dismutase and catalase activities showed a peak of activity after 7 days of acclimatisation, suggesting a protection against reactive oxygen species. Peroxidase activity increased about 2-fold after 2 days of acclimatisation and remained high until day 14, probably linked to the cell wall stiffening and the lignification processes. In addition, a progressive increase in the photochemical quenching parameters and the electronic transport rate was observed, coupled with a decrease in the non-photochemical quenching parameters, which indicate a progressive photosynthetic efficiency during this process. Taken together, antioxidant enzymes, lipid peroxidation, and chlorophyll fluorescence are proven as suitable tools for the physiological state evaluation of micropropagated plants during acclimatisation to ex vitro conditions.

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Photosynthesis parameters during acclimatization of in vitro-grown olive plantlets

Cited by 11 publications

References 26 publications

Synthesis and effect of a new Terbium gibberellic complex on the histopathological alteration induced by Gibberellic acid on liver and kidney of mice Mus musculus

Synthesis and effect of a new Terbium gibberellic complex on the histopathological alteration induced by Gibberellic acid on liver and kidney of mice Mus musculus

Direct Shoot Organogenesis and Clonal Fidelity Confirmation of Tongkat Ali (Eurycoma longifolia) using Molecular Markers

Antioxidant Metabolism and Chlorophyll Fluorescence during the Acclimatisation to Ex Vitro Conditions of Micropropagated Stevia rebaudiana Bertoni Plants

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