Photosynthetic Activity and Cellular Integrity of the Andean Legume Pachyrhizus ahipa (Wedd.) Parodi under Heat and Water Stress

Matos, Mauro; Campos, Paula Scotti; Ramalho, José C.; Medeira, M.C.; Maia, Miguel; Semedo, José N.; Marques, N.; Matos, Anabela

doi:10.1023/a:1024331414564

Cited by 27 publications

(16 citation statements)

References 36 publications

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“…Based on our findings that higher drought tolerance is acquired following increasing the ratio of 18:3 to 18:2, we propose that the decrease in 18:3 observed earlier under drought conditions (Dakhma et al. , 1995; Matos et al. , 2002; Olsson, 1995) reflects damage, whereas the few cases where increased levels of 18:3 were found under drought stress (Repellin et al.…”

Section: Discussionmentioning

confidence: 84%

Modulated fatty acid desaturation via overexpression of two distinctω‐3 desaturases differentially alters tolerance to various abiotic stresses in transgenic tobacco cells and plants

et al. 2005

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SummaryChanges in the degree of fatty acid (FA) desaturation are implicated in plant responses to various abiotic stresses, including heat, salt and drought. However, it is still not known whether decreased levels of linolenic acid, found in many plants subjected to salt and drought stress, reflect a mechanism of defence or damage. We addressed this question by generating tobacco cells and plants ectopically overexpressing two FA desaturases: the cytosolic FAD3 or the plastidic FAD8. A remarkable increase in the ratio of total linolenic to linoleic acids resulted from overexpression of FAD3, whereas ectopic overexpression of FAD8 induced an increased ratio mainly in the plastidic lipids. Here we present evidence that overexpressing FAD8 imposes much greater heat sensitivity than does FAD3 overexpression, in both cultured cells and whole plants. Overexpression of either FAD3 or FAD8 increases tolerance to drought in tobacco plants and to osmotic stress in cultured cells. These findings suggest that a drought-induced decreased level of linolenic acid reflects damage. Our results point to the potential of exploiting FAD overexpression as a tool to ameliorate drought tolerance.

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Section: Discussionmentioning

confidence: 84%

Modulated fatty acid desaturation via overexpression of two distinctω‐3 desaturases differentially alters tolerance to various abiotic stresses in transgenic tobacco cells and plants

et al. 2005

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“…Membrane fluidity can affect ATPase activity ( Cooke and Burden, 1990 ; Liu et al, 2008 ), bilayer permeability ( Schuler et al, 1991 ), and carrier-mediated transport ( Deuticke and Haest, 1987 ), while membrane fluidity and structure are affected by membrane lipid composition and the desaturation degree of fatty acid ( Mikami and Murata, 2003 ). Many earlier studies have reported that changes in the unsaturated fatty acid content can change plant tolerance to different conditions such as cold, drought, heat, and salt ( Dakhma et al, 1995 ; Olsson, 1995 ; Matos et al, 2002 ; Sui et al, 2007b , c ; Liu et al, 2008 ; Sui and Han, 2014 ). The membrane of plants consists of lipids, proteins and sugars.…”

Section: Introductionmentioning

confidence: 99%

Overexpression of Glycerol-3-Phosphate Acyltransferase from Suaeda salsa Improves Salt Tolerance in Arabidopsis

et al. 2017

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Glycerol-3-phosphate acyltransferase is the first acyl esterifying enzyme in phosphatidylglycerol (PG) synthesis process. In this study, we isolated and characterized the glycerol-3-phosphate acyltransferase (GPAT) gene from Suaeda salsa (S. salsa) and obtained the full length of the GPAT gene from S. salsa (SsGPAT) by 5′ and 3′ RACE. The clone contained an open reading frame (ORF) of 1167 bp nucleotides that comprised of 388 amino acid residues. Real-time PCR revealed that the mRNA accumulation of GPAT in S. salsa was induced by salt stress. The highest expression levels were observed when S. salsa leaves were exposed to 300 mM NaCl treatment. At the germination stage, the germination rate and root length of overexpressed Arabidopsis strains were significantly higher than WT under different concentrations of NaCl treatments, while the inhibitory effect was significantly severe in T-DNA insertion mutant strains. In the seedling stage, chlorophyll content, the photochemical efficiency of PSII, PSI oxidoreductive activity (ΔI/Io), and the unsaturated fatty acid content of PG decreased less in overexpressed strains and more in mutant strains than that in WT under salt stress. These results suggest that the overexpression of SsGPAT in Arabidopsis enhances salt tolerance and alleviates the photoinhibition of PSII and PSI under salt stress by improving the unsaturated fatty acid content of PG.

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“…Moreover, drought stress was found to reduce linolenic acid (% of total FAs) in other plants such as rape leaves (Brassica napus L.) (Dakhma et al, 1995), Andean bean [Pachyrhizus ahipa (Wedd.) Parodi] (Matos et al, 2002), and pea (Pisum sativum L.) (Olsson, 1995). However, an increase in 18:3 or other unsaturated FAs was also reported in chloroplast lipids under drought stress in coconut palm (Cocos nucifera L.) leaves (Repellin et al, 1997), sunflower seedlings (Helianthus annuus L.) (Sgherri et al, 1996), and grape leaves (Vitis vinifera L.) (Toumi et al, 2008).…”

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confidence: 99%

Changes of Leaf Membrane Fatty Acid Composition and Saturation Level of Warm‐Season Turfgrass during Drought Stress

et al. 2017

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Although it is known that drought tolerance can be associated with membrane fatty acid (FA) unsaturation, a relevant study is lacking in zoysiagrass (Zoysia spp.) and St. Augustinegrass [Stenotaphrum secundatum (Walt.) Kuntze.]. Our objectives were (i) to substantiate that variation exists among species and genotypes for leaf tissue FA composition under well‐watered conditions; (ii) to evaluate the relationship between well‐watered leaf FA composition and turf performance under drought stress; and (iii) to determine if modifications in FA content occur during drought stress and if the changes associate with improved drought tolerance. Nine genotypes of three species were subjected to both controlled dry down and well‐watered conditions for 12 d in a greenhouse. Plants were evaluated for cumulative water use, transpiration response, turfgrass quality, and leaf FA composition (palmitic acid, stearic acid, palmitoleic acid, hexadecadienoic acid, oleic acid, linoleic acid, and linolenic acid). St. Augustinegrass had a constitutively higher unsaturation FA fraction than two zoysiagrass species, owing to its higher linolenic acid and low palmitic acid fractions. During dry down, relative FA contents remained stable and no early unsaturation alteration was found. Two genotypes, ‘UF182’ Japanese lawngrass (Z. japonica Steud.) and ‘Zeon’ manilagrass [Z. matrella (L.) Merr.], displayed an increase in unsaturated FAs when severe drought stress occurred (rapid decline in turfgrass quality), which was associated with good turfgrass quality and apparent drought tolerance. The result in zoysiagrass supports previous reports suggesting increased levels of linolenic acid under drought stress to be a defensive mechanism.

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Photosynthetic Activity and Cellular Integrity of the Andean Legume Pachyrhizus ahipa (Wedd.) Parodi under Heat and Water Stress

Cited by 27 publications

References 36 publications

Modulated fatty acid desaturation via overexpression of two distinctω‐3 desaturases differentially alters tolerance to various abiotic stresses in transgenic tobacco cells and plants

Modulated fatty acid desaturation via overexpression of two distinctω‐3 desaturases differentially alters tolerance to various abiotic stresses in transgenic tobacco cells and plants

Overexpression of Glycerol-3-Phosphate Acyltransferase from Suaeda salsa Improves Salt Tolerance in Arabidopsis

Changes of Leaf Membrane Fatty Acid Composition and Saturation Level of Warm‐Season Turfgrass during Drought Stress

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