Photosynthetic biomineralization of radioactive Sr via microalgal CO2 absorption

Lee, Seung Yeop; Jung, Kwang‐Hwan; Lee, Ju Eun; Lee, Keon Ah; Lee, Sang-Hyo; Lee, Ji Young; Lee, Jae Kwang; Jeong, Jong Tae; Lee, Seung-Yop

doi:10.1016/j.biortech.2014.09.023

Cited by 26 publications

(8 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several organisms have been screened for their ability to (co)-precipitate Sr. For example, the microalga Chlorella vulgaris, soil bacterium Sporosarcina pasteurii, and the bacterium Halomonas sp. induce co-precipitation of strontianite (SrCO 3 ) within extracellularly precipitated CaCO 3 mineral phase; the desmid Closterium moniliferum forms celestite (SrSO 4 ) within its vacuole by concentrating preferentially Sr and Ba over Ca. − More recently, Cam et al showed that stable-Sr is selectively accumulated within intracellular ACC and PolyP inclusions in the cyanobacterium G. lithophora .…”

Section: Introductionmentioning

confidence: 99%

Sequestration of Radionuclides Radium-226 and Strontium-90 by Cyanobacteria Forming Intracellular Calcium Carbonates

Mehta

Benzerara

Kocar

et al. 2019

Environ. Sci. Technol.

View full text Add to dashboard Cite

226 Ra is a naturally occurring radionuclide with a half-life of 1600 y. In 14 contrast, 90 Sr is a radionuclide of sole anthropogenic origin, produced by nuclear fission 15 reactions and has a half-life of 29 y; each of these radionuclides poses potential threats to 16 human and ecosystem health. Here, the cyanobacterium G. lithophora, capable of forming 17 intracellular amorphous calcium carbonate inclusions, was investigated for its ability to 18 uptake 226 Ra and 90 Sr. In BG-11 medium, G. lithophora accumulated 3.9 µg g -1 226 Ra within 19 144 h and 47.9 ng g -1 90 Sr within 1 h, corresponding to ~99% removal of trace radionuclides. 20 The presence of high concentration Ca 2+ in the background media solution did not inhibit 21 90 Sr and 226 Ra uptake by G. lithophora. In contrast, dead biomass of G. lithophora 22 accumulated 0.8 µg g -1 226 Ra and 8.87 ng g -1 90 Sr. Moreover, Synechocystis, a non-23 biomineralizing cyanobacteria removed only 14% and 25% of 226 Ra and 90 Sr, respectively. 24 This suggested that sequestration of 90 Sr and 226 Ra was not intrinsic to all cyanobacteria but 25 was likely a specific biological trait of G. lithophora related to the formation of intracellular 26 amorphous Ca-carbonates. The unique ability of G. lithophora to uptake 90 Sr and 226 Ra at 27 2 high rates makes it an attractive candidate for further studies involving bioremediation of 28 these radionuclides. 29 30 31 TOC image 32 33 34 Ba, with Ba/Ca and Sr/Ca atomic ratios in inclusions higher by factors of 1370 and 90 48 respectively relative to the solutions in which cells grew 3,4 . Recently, it was confirmed that 49 cyanobacteria capable of forming intracellular ACC contained a much higher content of50 alkaline earth elements (AEE) than all other cyanobacteria 5 . In addition to intracellular ACC 51 inclusions, G. lithophora also forms intracellular polyphosphate inclusions (PolyP), 52 polymers of orthophosphate which may serve as reserves of P and/or energy for cells 6 . 53 Using stable isotopes, Cam et al. showed that G. lithophora preferentially accumulated Ba 54 over Sr and finally Ca within intracellular PolyP and ACC. Currently, the origin of such a 55 surprising selectivity remains unclear. The proven ability of G. lithophora to selectively 56 sequester Sr over Ca within intracellular inclusions provides an intriguing microbial 57 framework to examine if this ability extends to radioactive AEE such as radium ( 226 Ra) and 58 radio-strontium ( 90 Sr). In the environment, 90 Sr and 226 Ra are present at trace concentrations, 59 along with excess dissolved Ca. Whether the observed selective accumulation of AEE is 60 extended to 90 Sr and 226 Ra at such low concentrations and in the presence of high 61 concentrations of Ca remains to be explored. 62 Radium-226 is a naturally occurring radioactive alkaline earth element (AEE) with a 63 half-life of 1600 y. It is one of the dominant soluble radionuclides present in groundwater 7 . 64 In the United States, 226 Ra activity in drinking water is regula...

show abstract

Section: Introductionmentioning

confidence: 99%

Sequestration of Radionuclides Radium-226 and Strontium-90 by Cyanobacteria Forming Intracellular Calcium Carbonates

Mehta

Benzerara

Kocar

et al. 2019

Environ. Sci. Technol.

View full text Add to dashboard Cite

show abstract

“…24 Several organisms able to trap Sr and/or Ba have been discovered, including some bacteria, micro-algae and plants. 11,[25][26][27][28][29][30][31][32][33][34][35] Different mechanisms of sequestration may be involved. First, Sr 2+ and Ba 2+ may be adsorbed on the cell surface.…”

Section: Introductionmentioning

confidence: 99%

Selective Uptake of Alkaline Earth Metals by Cyanobacteria Forming Intracellular Carbonates

Cam

Benzerara

Georgelin

et al. 2016

Environ. Sci. Technol.

View full text Add to dashboard Cite

The uptakes of calcium (Ca), strontium (Sr), and barium (Ba) by two cyanobacterial strains, Cyanothece sp. PCC7425 and Gloeomargarita lithophora, both forming intracellular carbonates, were investigated in laboratory cultures. In the culture medium BG-11 amended with 250 μM Ca and 50 or 250 μM Sr and Ba, G. lithophora accumulated first Ba, then Sr, and finally Ca. Sr and Ba were completely accumulated by G. lithophora cells at rates between 0.02 and 0.10 fmol h cell and down to extracellular concentrations below the detection limits of inductively coupled plasma atomic emission spectroscopy. Accumulation of Sr and Ba did not affect the growth rate of the strain. This sequential accumulation occurred mostly intracellularly within polyphosphate and carbonate granules and resulted in the formation of core-shell structures in carbonates. In contrast, Cyanothece sp. PCC7425 showed neither a preferential accumulation of heavier alkaline earth metals nor core-shell structures in the carbonates. This indicated that fractionation between alkaline earth metals was not inherent to intracellularly calcifying cyanobacteria but was likely a genetically based trait of G. lithophora. Overall, the capability of G. lithophora to sequester preferentially Sr and Ba at high rates may be of considerable interest for designing new remediation strategies and better understanding the geochemical cycles of these elements.

show abstract

“…The decrease of the membrane potential dependence K + from ingressed Ca 2+ also proves the selective absorbtion of Ca 2+ ions. It is known, that Chara algae are inclined to accumulate ions (metals and, especially, Ca 2+ ) in alkaline pH and availability of carbonates in solution [16][17][18]. However, the pH of solution used in experiment were lower than 8.…”

Section: Resultsmentioning

confidence: 96%

THE ACCUMULATION PROCESS of¹³⁷Cs AND⁹⁰Sr IN THE CELL OF NITELLOPSIS OBTUSA ALGAE

Marčiulionienė¹,

Jefanova²,

Sakalauskas³

et al. 2016

RadJ

View full text Add to dashboard Cite

Abstract. In the present study, we investigated the accumulation of 137 Cs and 90 Sr in compartments of the Nitellopsis obtusa cells. The effect of Sr 2+ , Cs + and Ca 2+ , K + , which are chemical analogues of 90 Sr and 137 Cs, to the bioelectric parameters of these algae were studied simultaneously. The aim of this work was studying the penetration of 137 Cs and 90 Sr through regulating membrane barriers in the cells of starry stonewort (Nitellopsis obtusa). 137 Cs and 90 Sr are accumulated mainly in the cell membrane (75% to 92%) of these algae. The cell membrane as a cation exchanger regulates ion flow through the first cells diffusion barrier -its thick outer cytomembrane (the complex consisting of the cell wall and plasmalemma). Significantly, smaller amounts of 137 Cs and 90 Sr enter into the cytoplasm than in the cytomembrane, 10-20% and 3-10%, respectively. Analysis of the accumulation levels of 137 Cs and 90 Sr in the compartments of the Nitellopsis obtusa cells show their accumulation in the cell membrane as well as their active transport through outer and inner cytoplasmic membranes. Membrane potentials determined mainly by the gradient of the K + ions are doing an important regulatory function in this process. From the obtained data it results that cells of Nitellopsis obtusa algae can be a convenient radioecological model for the study of the accumulation of radionuclides in plants at the cellular level.

show abstract

Photosynthetic biomineralization of radioactive Sr via microalgal CO2 absorption

Cited by 26 publications

References 19 publications

Sequestration of Radionuclides Radium-226 and Strontium-90 by Cyanobacteria Forming Intracellular Calcium Carbonates

Sequestration of Radionuclides Radium-226 and Strontium-90 by Cyanobacteria Forming Intracellular Calcium Carbonates

Selective Uptake of Alkaline Earth Metals by Cyanobacteria Forming Intracellular Carbonates

THE ACCUMULATION PROCESS of¹³⁷Cs AND⁹⁰Sr IN THE CELL OF NITELLOPSIS OBTUSA ALGAE

Contact Info

Product

Resources

About

Photosynthetic biomineralization of radioactive Sr via microalgal CO2 absorption

Cited by 26 publications

References 19 publications

Sequestration of Radionuclides Radium-226 and Strontium-90 by Cyanobacteria Forming Intracellular Calcium Carbonates

Sequestration of Radionuclides Radium-226 and Strontium-90 by Cyanobacteria Forming Intracellular Calcium Carbonates

Selective Uptake of Alkaline Earth Metals by Cyanobacteria Forming Intracellular Carbonates

THE ACCUMULATION PROCESS of137Cs AND90Sr IN THE CELL OF NITELLOPSIS OBTUSA ALGAE

Contact Info

Product

Resources

About

THE ACCUMULATION PROCESS of¹³⁷Cs AND⁹⁰Sr IN THE CELL OF NITELLOPSIS OBTUSA ALGAE