Photosynthetic capacity, growth and water relations in ‘Golden’ papaya cultivated in vitro with modifications in light quality, sucrose concentration and ventilation

Schmildt, Omar; Netto, Alena Torres; Schmildt, Edílson Romais; Carvalho, Virgínia Silva; Otoni, Wagner Campos; Campostrini, Eliemar

doi:10.1007/s40626-014-0026-y

Cited by 16 publications

(8 citation statements)

References 51 publications

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“…by changing the osmotic balance. There are even references about sucrose toxicity in high dosages for certain plants (Schmildt et al ). Thus, the question arises, why not just remove or reduce the sucrose supply during in vitro cultivation?…”

Section: Discussionmentioning

confidence: 99%

Mixotrophic in vitro cultivations: the way to go astray in plant physiology

Ševčı́ková

Lhotáková

Hamet

et al. 2019

Physiologia Plantarum

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Rate of photosynthesis and related plant carbohydrate status are crucial factors affecting plant vigor. Sugars providing carbon and energy sources serve also as important signaling molecules governing plant growth and development through a complex regulatory network. These facts are often neglected when mixotrophic cultivation of plants in vitro is used, where artificial exogenous sugar supply hinders studies of metabolism as well as sugar‐driven developmental processes. We compared the growth, selected gas‐exchange parameters and sugar metabolism characteristics in four model plants, potato (Solanum tuberosum ‘Lada’), tobacco (Nicotiana tabacum ‘Samsun’), rapeseed (Brassica napus ‘Asgard’) and strawberry (Fragaria vesca), under both photomixotrophic (PM) and photoautotrophic (PA) conditions. To ensure PA conditions, we used our improved sun caps that serve as gas and light permeable covers for cultivation vessels. We found bigger biomass accumulation, larger leaf areas, higher stomatal conductance and higher instantaneous water use efficiency and lower root sugar contents in PA plants compared to PM ones. However, for other characteristics (root biomass, root/shoot ratio, pigment contents, leaf sugar and starch levels and transpiration rates), a strong species‐dependent reactions to the exogenous sugar supply was noted, which does not allow to create a general view on the overall impact of PM nutrition under in vitro conditions.

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Section: Discussionmentioning

confidence: 99%

Mixotrophic in vitro cultivations: the way to go astray in plant physiology

Ševčı́ková

Lhotáková

Hamet

et al. 2019

Physiologia Plantarum

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“…Our results were similar to those of previous studies; the root fresh mass and dry mass of triticale decreased under salt stress, the pretreatment with glucose and sucrose could maintain or enhance these traits, and the pretreatment with mannitol did not relieve the reductions of root fresh mass and dry mass. Schmildt et al (2015) found that the increased production of papaya dry biomass was caused by the application of an exogenous carbon source. Campostrini et al (2008) also found that shoot dry matter was increased by sucrose in the culture medium.…”

Section: Discussionmentioning

confidence: 99%

Effects of exogenous glucose and sucrose on photosynthesis in triticale seedlings under salt stress

Wang¹,

Li²,

Wei³

et al. 2019

Photosynt.

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Salinization disturbs metabolic processes of plants and results in reduced growth and productivity. In our study, the Dongnong 8809 variety of triticale (×Triticosecale Wittmack) was evaluated for its physiological responses to salt stress during the seedling stage. We evaluated biomass production, relative chlorophyll content, chlorophyll fluorescence, and leaf gas-exchange parameters. Our results indicated that salt stress greatly reduced matter accumulation, SPAD value, photosynthetic capacity. However, seedlings treated with exogenous sugars showed significantly enhanced matter accumulation, SPAD value, quantum yield of PSII, electron transport rate, net photosynthetic rate, decreased stomatal limitation value, and improved photosynthetic capacity compared to seedlings treated with salt stress alone. Exogenous glucose and sucrose can alleviate the inhibitory effect of salt stress on the growth of triticale, and of the two exogenous sugars, sucrose had a stronger alleviating effect than that of glucose under salt stress. rate; ETR -electron transport rate; F0 -minimal fluorescence yield of the dark-adapted state; F0' -minimal fluorescence yield of the light-adapted state; Fm -maximal fluorescence yield of the dark-adapted state; Fm' -maximal fluorescence yield of the light-adapted state; Fs -steady-state fluorescence yield; Fv/Fm -maximum quantum yield of PSII photochemistry; Fv/F0 -potential photochemical efficiency; gs -stomatal conductance; Ls -stomatal limitation value; NPQ -nonphotochemical quenching; PN -net photosynthetic rate; qP -photochemical quenching coefficient; WUEwater-use efficiency; WUEi -intrinsic water-use efficiency; Y -quantum yield of PSII.

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“…However, in studies carried out on other species, subjected to a 16 h photoperiod, low concentration of CO 2 into the vessels was observed: Pfaffia glomerata [ 246 ], Solanum tuberosum [ 247 , 248 ], Carica papaya [ 249 ], Castanea sativa [ 227 ], Vitis vinifera [ 250 , 251 ], Fragaria x ananassa [ 252 ], Hyptis marrubioides and Hancornia speciosa [ 253 ].…”

Section: Effects Of Photoperiodmentioning

confidence: 99%

Light and Plant Growth Regulators on In Vitro Proliferation

Cavallaro

Pellegrino

Muleo

et al. 2022

Plants

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Plant tissue cultures depend entirely upon artificial light sources for illumination. The illumination should provide light in the appropriate regions of the electromagnetic spectrum for photomorphogenic responses and photosynthetic metabolism. Controlling light quality, irradiances and photoperiod enables the production of plants with desired characteristics. Moreover, significant money savings may be achieved using both more appropriate and less consuming energy lamps. In this review, the attention will be focused on the effects of light characteristics and plant growth regulators on shoot proliferation, the main process in in vitro propagation. The effects of the light spectrum on the balance of endogenous growth regulators will also be presented. For each light spectrum, the effects on proliferation but also on plantlet quality, i.e., shoot length, fresh and dry weight and photosynthesis, have been also analyzed. Even if a huge amount of literature is available on the effects of light on in vitro proliferation, the results are often conflicting. In fact, a lot of exogenous and endogenous factors, but also the lack of a common protocol, make it difficult to choose the most effective light spectrum for each of the large number of species. However, some general issues derived from the analysis of the literature are discussed.

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Photosynthetic capacity, growth and water relations in ‘Golden’ papaya cultivated in vitro with modifications in light quality, sucrose concentration and ventilation

Cited by 16 publications

References 51 publications

Mixotrophic in vitro cultivations: the way to go astray in plant physiology

Mixotrophic in vitro cultivations: the way to go astray in plant physiology

Effects of exogenous glucose and sucrose on photosynthesis in triticale seedlings under salt stress

Light and Plant Growth Regulators on In Vitro Proliferation

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