Photosynthetic carbon assimilation of transgenic tobacco plant expressing cytoplasmic carbonic anhydrase cDNA from mouse.

Transgenic improvement of photosynthetic property in tobacco using E12 .OMEGA. promoter system to express higher level of mouse carbonic anhydrase activity in cytoplasm.

“…In the previous report (Jinushi et al ., 1998), based on a very low level of cytoplasmic intrinsic CA activity we generated transgenic tobacco (fCA plants) which expresses foreign mouse CA in the mesophyll cytoplasm controlled by a conventional cauliflower mosaic virus 35S (CaMV 35S) promoter. The fCA plants which expressed foreign CA at high levels showed a significant and consistent enhancing tendency in the photosynthetic carbon assimilation (PCA) rate under lower CO2 conditions.…”

“…Among the unique techniques available two reports were suggestive; (1) Kay et al (1987) reported that the duplication of CaMV35S promoter sequences enhanced foreign gene expressions. (2) Mitsuhara et al (1996) (Jinushi et al, 1998;Horsch et al, 1985). blot analysis of the leaf extracts of individuals of 4 strains (ffCA -1, -3, -6 and -7) among the 14 seedlings recovered from the screening antibiotic Km medium.…”