Photosynthetic light reactions increase total lipid accumulation in carbon-supplemented batch cultures of Chlorella vulgaris

Woodworth, Benjamin D.; Mead, Rebecca L.; Nichols, Courtney; Kolling, Derrick R.J.

doi:10.1016/j.biortech.2014.11.098

Cited by 33 publications

(37 citation statements)

References 22 publications

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“…Woodworth et al [ 31 ] found that the biomass of C . vulgaris cultured under mixotrophy was higher than that under autotrophy at the early stage following supplementation with glucose (20g/L), whereas the biomasses of heterotrophic and mixotrophic cultures were nearly the same at the saturated stage.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Chlorella sorokiniana growth properties in monosaccharide-supplemented batch culture

et al. 2018

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To reveal growth properties of Chlorella sorokiniana UTEX 1230, four monosaccharides (glucose, fructose, galactose and xylose) were individually supplemented into medium as carbon sources for the cultivation of C. sorokiniana UTEX 1230. Supplementation with glucose increased OD750, biomass and lipid yield but decreased protein abundance per unit dry weight of biomass under all concentrations examined, the maximum OD750, biomass and lipid yield increased 2.04, 6.78 and 12.43 times, respectively, compared with autotrophic controls. A low concentration of glucose (<4 g/L) simultaneously promoted the biosynthesis of chlorophylls and protein abundance per unit culture volume, but decreased the lipid content per unit dry weight of biomass and all supplemented glucose can be exhausted within 7 days. Higher glucose concentrations (≥4 g/L) decreased the biosynthesis of chlorophylls and protein abundance per unit culture volume, but increased the lipid content per unit dry weight of biomass. In glucose supplemented scenario, C. sorokiniana UTEX 1230 growth was light-independent. Supplementation with fructose promoted C. sorokiniana UTEX 1230 growth to a much lesser extent compared with glucose, whereas supplementation with galactose had no effect and supplementation with xylose even inhibited growth. Our findings represent basic experimental data on the effect of monosaccharides and can serve as the basis for a robust cultivation system to increase biomass and lipid yield.

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Section: Discussionmentioning

confidence: 99%

Characterization of Chlorella sorokiniana growth properties in monosaccharide-supplemented batch culture

et al. 2018

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“…Most of the feeding modes in the previous studies are based on batch operations, where microalgae will be cultivated for a certain period. The batch culture of microalgae is mature and technology‐ready, and it has been used in many microalgal species, such as Chlorella zofingiensis , Chlorella vulgaris , Scenedesmus sp., Nannochloropsis salina , Chlamydomonas polypyrenoideum , Cyclotella sp . and many others, ranging from freshwater microalgae to marine microalgae.…”

Section: Microalgal Cultivation Strategiesmentioning

confidence: 99%

Microalgal culture strategies for biofuel production: a review

Zhu

2015

Biofuels Bioprod Bioref

154

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In response to the energy crisis, global warming and climate change, microalgae have received a lot of attention as a promising feedstock for the production of biofuels. However, microalgal biofuels are still unaffordable due to low productivity and their substantial requirements in capital investment and operation costs. Much original research regarding the various microalgal cultivation strategies has been reported, in an attempt to promote microalgal biofuel production. However, current literature indicates the fragmented nature of information available regarding the strategies for the improvement of microalgal production for biofuel conversion. From a systematic perspective, this review highlights the main microalgal cultivation strategies for the achievement of improved biomass and biofuel productivity. It first discloses the current state of microalgal production as a biofuel feedstock, giving general introduction to the topic. Subsequently, it summarizes the current microalgae cultivation technology. Microalgal cultivation strategies are then discussed systematically, including integration with wastewater treatment and CO2 mitigation, batch vs. semi‐continuous vs. continuous culture, two‐stage continuous cultivation, co‐culture, stress culture, and microalgal cultivation with harvest water recycling. Finally, concluding remarks are put forward. © 2015 Society of Chemical Industry and John Wiley & Sons, Ltd

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“…Hence, it would appear that the best use of low or no cost organic carbon substrates would be their use in supporting mixotrophic growth of microalgae. In at least some cases there appears to be a synergistic effect, with higher growth, biomass yields, and lipid productivities being obtained with mixotrophic growth than with photoautotrophic growth (Heredia-Arroyo et al, 2011; Lin et al, 2015;Woodworth et al, 2015). In fact, mixotrophic growth on glycerol can even exceed that seen with heterotrophic growth (Leite et al, 2015;Yeh and Chang, 2012).…”

Section: Introductionmentioning

confidence: 99%

Effect of nitrogen regime on microalgal lipid production during mixotrophic growth with glycerol

Paranjape

Leite

Hallenbeck

2016

Bioresource Technology

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Mixotrophic growth of microalgae to boost lipid production is currently under active investigation. Such a process could be of practical importance if a cheap source of organic carbon, such as waste glycerol from biodiesel production, could be used. Several previous studies have already demonstrated that this carbon source can be used by different indigenous strains of microalgae. In this study it is shown that different nitrogen limitation strategies can be applied to further increase lipid production during growth with glycerol. In one strategy, cultures were grown in nitrogen replete medium and then resuspended in nitrogen free medium. In a second strategy, cultures were grown with different initial concentrations of nitrate. Lipid production by the two microalgal strains used, Chlorella sorokiniana (PCH02) and Chlorella vulgaris (PCH05), was shown to be boosted by strategies of nitrogen limitation, but they responded differently to how nitrogen limitation was imposed.

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Photosynthetic light reactions increase total lipid accumulation in carbon-supplemented batch cultures of Chlorella vulgaris

Cited by 33 publications

References 22 publications

Characterization of Chlorella sorokiniana growth properties in monosaccharide-supplemented batch culture

Characterization of Chlorella sorokiniana growth properties in monosaccharide-supplemented batch culture

Microalgal culture strategies for biofuel production: a review

Effect of nitrogen regime on microalgal lipid production during mixotrophic growth with glycerol

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