Photosynthetic performance and stevioside concentration are improved by the arbuscular mycorrhizal symbiosis in <i>Stevia rebaudiana</i> under different phosphate concentrations

Sarmiento-López, Luis G.; López-Meyer, Melina; Sepúlveda-Jiménez, Gabriela; Cárdenas, Luis; Rodrı́guez-Monroy, Mario

doi:10.7717/peerj.10173

Cited by 9 publications

(5 citation statements)

References 54 publications

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“…AMF are important components of soil biological processes and functional links between plants and soil ( Yang et al, 2010 ). Mycorrhizal fungi have a vital impact on the composition of microbial and plant communities ( van der Heijden et al, 2015 ; Genre et al, 2020 ), and AMF symbiosis can also improve nutrient and water supply to host plants ( Parniske, 2008 ; Song et al, 2015 ; Wang, Pokharel & Chen, 2019 ; Sarmiento-López et al, 2020 ; Shi et al, 2020a ). Positive plant water relations by AMF have been demonstrated to improve plant drought resistance ( Chen et al, 2020 ) by enhancing the uptake of N and P under drought stress ( Hashem et al, 2018 ), alleviate soil water stress ( Mickan et al, 2016 ), and promote plants to deplete soil moisture to alleviate plant water stress ( Hardie, 1985 ).…”

Section: Introductionmentioning

confidence: 99%

Alterations to arbuscular mycorrhizal fungal community composition is driven by warming at specific elevations

Yang

Shi

Mickan

et al. 2021

PeerJ

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Background Global warming can alter plant productivity, and community composition which has consequences for soil-plant associated microorganisms. Arbuscular mycorrhizal fungi (AMF) are distributed widely and form symbiotic relationships with more than 80% of vascular plants and play a key role in nutrient cycling processes at the ecosystem scale. Methods A simulated warming experiment at multiple elevations (3,000, 3,500, 3,800, and 4,170 m) was conducted utilizing an in-situ open-top chamber (OTC) for exploring the effect of global warming on AMF community structure in the Qinghai-Tibet Plateau (QTP). This region has been identified as one of the most sensitive areas to climatic changes. Soil DNA was extracted and sequenced using next the Mi-Seq platform for diversity profiling. Results AMF richness was higher under the simulated warming chamber, however this only occurred in the elevation of 3,500 m. Warming did not alter other AMF alpha diversity indices (e.g. Shannon, Ace, and Simpson evenness index). Glomus and Acaulospora were the dominate AMF genera as assessed through their relative abundance and occurrence in control and warming treatments at the different elevations. Conclusion Warming changed significantly AMF community. The effects of warming on AMF community structure varied depend on elevations. Moreover, the occurrences of AMF in different genera were also presented the different responses to warming in four elevations.

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Section: Introductionmentioning

confidence: 99%

Alterations to arbuscular mycorrhizal fungal community composition is driven by warming at specific elevations

Yang

Shi

Mickan

et al. 2021

PeerJ

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“…The arbuscular percentage was calculated with MycoCalc software ( https://www2.dijon.inrae.fr/mychintec/Mycocalcprg/download.html ). The number of infection sites, appressoria, vesicles and arbuscules along the length of each root was determined by image analysis using ImageJ software (ImageJ, version 1.8.0_112) as previously described ( Sarmiento-Lopez et al., 2020 ). For each plant, 30 root segments colonized by R. irregularis were assessed.…”

Section: Methodsmentioning

confidence: 99%

TETRASPANIN 8-1 from Phaseolus vulgaris plays a key role during mutualistic interactions

Parra-Aguilar,

Sarmiento-López,

Santana

et al. 2023

Front. Plant Sci.

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Arbuscular mycorrhizal (AM) fungi and rhizobia form two of the most important plant-microbe associations for the assimilation of phosphorus (P) and nitrogen (N). Symbiont-derived signals are able to coordinate the infection process by triggering multiple responses in the plant root, such as calcium influxes and oscillations, increased reactive oxygen species (ROS), cytoskeletal rearrangements and altered gene expression. An examination was made of the role of tetraspanins, which are transmembrane proteins that self-organize into tetraspanin web regions, where they recruit specific proteins into platforms required for signal transduction, membrane fusion, cell trafficking, and ROS generation. In plant cells, tetraspanins are scaffolding proteins associated with root radial patterning, biotic and abiotic stress responses, cell fate determination, plasmodesmata and hormonal regulation. Some plant tetraspanins, such as Arabidopsis thaliana TETRASPANIN 8 and TETRASPANIN 9 (AtTET8 and AtTET9) are associated with exosomes during inter-kingdom communication. In this study, a homolog of AtTET8, PvTET8-1, in common bean (Phaseolus vulgaris L. var. Negro Jamapa) was examined in roots during interactions with Rhizobium tropici and Rhizophagus irregularis. The promoter of PvTET8-1 contained several cis-acting regulatory DNA elements potentially related to mutualistic interactions, and PvTET8-1 was transcriptionally activated during AM fungal and rhizobial associations. Silencing it decreased the size and number of nodules, nitrogen fixation, and mycorrhizal arbuscule formation, whereas overexpressing it increased the size and number of nodules, and mycorrhizal arbuscule formation but decreased nitrogen fixation. PvTET8-1 appears to be an important element in both of these mutualistic interactions, perhaps through its interaction with NADPH oxidase and the generation of ROS during the infection processes.

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“…Afterward, rebaudioside-A is formed by the addition of a D-glucose molecule of stevioside, catalyzed by the UGT76G1 enzyme [ 11 ]. There are a few reports available on the impact of biomolecules, such as gibberellin, salicylic acid (SA), and chitosan, on gene expression associated with SGs biosynthesis in stevia crop [ 10 , 11 ] Some work has been done on the direct growth promotion of stevia using arbuscular mycorrhizal fungi (AMF) [ 12 ]. However, there is no research available concerning the role of plant growth-promoting rhizobacteria (PGPR) in the regulation of SGs, metabolite content, growth, biochemical aspects, and management of phytopathogens of stevia.…”

Section: Introductionmentioning

confidence: 99%

A Novel Bacillus safensis-Based Formulation along with Mycorrhiza Inoculation for Controlling Alternaria alternata and Simultaneously Improving Growth, Nutrient Uptake, and Steviol Glycosides in Stevia rebaudiana under Field Conditions

Prakash

Egamberdieva

Arora

2022

Plants

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The excess use of chemicals by farmers in the agroecosystems degrades soil quality, disturbs soil ecology, and increases soil salinity and health hazards in humans. Stevia rebaudiana is an important medicinal and aromatic crop whose leaves contain steviol glycosides (SGs). The Bacillus safensis NAIMCC-B-02323 strain STJP from the rhizosphere of S. rebaudiana producing salicylic acid (16.80 µg/mL), chitinase (75.58 U/mL), β-1,3-glucanase (220.36 U/mL), and cellulase (170 U/mL) was taken as a plant growth-promoting rhizobacteria (PGPR). The cell-free supernatant (CFS) from strain STJP showed significant biocontrol activity against Alternaria alternata (80%), suggesting the protective role of extracellular metabolite(s) against phytopathogens. Paneer whey-based bioformulation (P-WBF) was developed to exploit B. safensis STJP to enhance the growth, nutrient uptake, soil properties, stevioside content, and SGs biosynthesis in S. rebaudiana under an A. alternata-infested field. The combined treatment of P-WBF and mycorrhiza (Glomus fasciculatum ABTEC) significantly enhanced plant growth parameters after 90 days, in comparison with control. The symbiotic action (P-WBF and mycorrhiza) displayed much better results in terms of chlorophyll a and b (improved by 132.85% and 39.80%, respectively), protein (by 278.75%), flavonoid (by 86.99%), carbohydrate (by 103.84%), antioxidant (by 75.11%), and stevioside (by 120.62%) contents in plants as compared to the untreated set. Further, the augmentation of potassium (by 132.39%), phosphorous (by 94.22%), and zinc (by 111.11%) uptake in plant tissues and soil was also observed by the application of P-WBF and mycorrhiza. The expression of UGT74G1 and UGT85C2 genes related to SG biosynthesis was upregulated (2.7- and 3.2-fold, respectively) in plants treated with P-WBF and mycorrhiza as further confirmed by the accumulation of SGs. The results suggest that the application of P-WBF and mycorrhiza not only provides an ecofriendly and sustainable solution to improve stevioside content in S. rebaudiana by a nutrient-linked mechanism but also paves the way to enhanced production of stevioside.

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Photosynthetic performance and stevioside concentration are improved by the arbuscular mycorrhizal symbiosis in Stevia rebaudiana under different phosphate concentrations

Cited by 9 publications

References 54 publications

Alterations to arbuscular mycorrhizal fungal community composition is driven by warming at specific elevations

Alterations to arbuscular mycorrhizal fungal community composition is driven by warming at specific elevations

TETRASPANIN 8-1 from Phaseolus vulgaris plays a key role during mutualistic interactions

A Novel Bacillus safensis-Based Formulation along with Mycorrhiza Inoculation for Controlling Alternaria alternata and Simultaneously Improving Growth, Nutrient Uptake, and Steviol Glycosides in Stevia rebaudiana under Field Conditions

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