Photosynthetic performance of Chlorella vulgaris R117 mass culture is moderated by diurnal oxygen gradients in an outdoor thin layer cascade

Rearte, Tomás Agustín; Celis-Plá, Paula S. M.; Neori, Amir; Masojídek, Jiří; Torzillo, Giuseppe; Gómez-Serrano, Cintia; Benavides, Ana Margarita Silva; Álvarez‐Gómez, Félix; Abdala-Díaz, Roberto; Ranglová, Karolína; Caporgno, Martín P.; Massocato, Thaís Fávero; Silva, Jaqueline Carmo da; Mahrouqui, Hafidh Al; Atzmüller, Richard; Figueroa, Félix L.

doi:10.1016/j.algal.2020.102176

Cited by 17 publications

(6 citation statements)

References 58 publications

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“…The pH values in the cultures roughly indicate CO 2 availability for growth [ 46 , 47 ]. In outdoor bioreactors, CO 2 might be supplied based on pH measurements keeping the value around 8 [ 8 , 48 , 49 ]. Without CO 2 addition, the photosynthetic activity causes an increase in the pH value up to 11 which may inhibit both microalgae and bacteria activity [ 50 ].…”

Section: Discussionmentioning

confidence: 99%

Photosynthesis Monitoring in Microalgae Cultures Grown on Municipal Wastewater as a Nutrient Source in Large-Scale Outdoor Bioreactors

Masojídek

Gómez-Serrano

Ranglová

et al. 2022

Biology

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Microalgae cultures were used for a WW treatment to remediate nutrients while producing biomass and recycling water. In these trials, raceway ponds (RWPs; 1 and 0.5 ha) were located next to a municipal (WW) treatment plant in Mérida, Spain. The ponds were used for continuous, all-year-round microalgae production using WW as a source of nutrients. Neither CO2 nor air was supplied to cultures. The objective was to validate photosynthesis monitoring techniques in large-scale bioreactors. Various in-situ/ex-situ methods based on chlorophyll fluorescence and oxygen evolution measurements were used to follow culture performance. Photosynthesis variables gathered with these techniques were compared to the physiological behavior and growth of cultures. Good photosynthetic activity was indicated by the build-up of dissolved oxygen concentration up to 380% saturation, high photochemical yield (Fv/Fm = 0.62–0.71), and relative electron transport rate rETR between 200 and 450 μmol e− m−2 s−1 at midday, which resulted in biomass productivity of about 15–25 g DW m−2 day−1. The variables represent reliable markers reflecting the physiological status of microalgae cultures. Using waste nutrients, the biomass production cost can be significantly decreased for abundant biomass production in large-scale bioreactors, which can be exploited for agricultural purposes.

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Section: Discussionmentioning

confidence: 99%

Photosynthesis Monitoring in Microalgae Cultures Grown on Municipal Wastewater as a Nutrient Source in Large-Scale Outdoor Bioreactors

Masojídek

Gómez-Serrano

Ranglová

et al. 2022

Biology

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“…Though the oxygen evolution is sometimes overlooked in large-scale microalgae cultures, high concentrations of dissolved oxygen (DO) concentrations in outdoor cultures occurring especially under high irradiance at midday in outdoor cultures can result in photoinhibition and photorespiration, which initiate a reduction in photosynthetic activity and growth. In open ponds, the build-up of DO concentrations several times over air saturation are frequently observed during the day, which usually inhibit the growth of many microalgae [97]. The maintenance of DO levels below this concentration requires degassing or culture mixing.…”

Section: Dissolved Oxygenmentioning

confidence: 99%

“…The culture heated in the early morning showed a significant increase in productivity of about 60% compared to the non-heated culture. The synergism of sub-optimal temperature and/or high DO concentrations (200-400% saturation) with excess irradiance can cause a decrease in photosynthetic activity and growth [63,97,[105][106][107]. It happens when the rise in light intensity is much faster than the rise in temperature, with a delay of hours, or the build-up of DO concentration due to high photosynthetic activity is enormous in a culture unit.…”

Section: Dissolved Oxygenmentioning

confidence: 99%

“…To optimize the yield of the CO 2 supply, it is recommended to operate on-demand injection of CO 2 at a pH close to the limiting value for photosynthesis to maximize the utilization while minimizing losses by decarbonisation [13]. In outdoor trials, using thin-layer cascades, CO 2 was supplied on the basis of pH measurements to keep the value of about 8 [97].…”

Section: Inorganic Carbonmentioning

confidence: 99%

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Variables Governing Photosynthesis and Growth in Microalgae Mass Cultures

et al. 2021

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Since the 1950s, microalgae have been grown commercially in man-made cultivation units and used for biomass production as a source of food and feed supplements, pharmaceuticals, cosmetics and lately biofuels, as well as a means for wastewater treatment and mitigation of atmospheric CO2 build-up. In this work, photosynthesis and growth affecting variables—light intensity, pH, CO2/O2 exchange, nutrient supply, culture turbulence, light/dark cell cycling, biomass density and culture depth (light path)—are reviewed as concerns in microalgae mass cultures. Various photosynthesis monitoring techniques were employed to study photosynthetic performance to optimize the growth of microalgae strains in outdoor cultivation units. The most operative and reliable techniques appeared to be fast-response ones based on chlorophyll fluorescence and oxygen production monitoring, which provide analogous results.

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“…; Celis-Plá et al 2021;Rearte et al 2021). Moreover, aspects obtained by uorescence parameters could infer culture performance associated with bio-optical properties of the organisms.…”

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confidence: 99%

Short-term nutrient removal efficiency by Ulva pseudorotundata (Chlorophyta): potential use for Integrated Multi-Trophic Aquaculture (IMTA)

Massocato

Robles-Carnero

Vega

et al. 2022

Preprint

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Short-term N-NH4+ and N-NO3- removal by U. pseudorotundata under different initial nutrient concentrations and biomass stocking densities, monitoring in parallel photosynthetic parameters obtained by Pulse-Amplitude-Modulation (PAM) Fluorometry of chlorophyll-a and evaluating biomass characteristics (CHN, proteins, carbohydrates) was investigated. The results showed that U. pseudorotundata cultivated under solar radiation in a land-based out-door pilot scale was efficient in removing N-NH4+ and N-NO3- even in concentrations as high as 500 µmol L⁻¹ ensuring complete water remediation in a period of 24 h and in all experimental conditions tested, although differences were observed depending on the stocking density and type of N-source. Treatments with N-NH4+ showed faster N removal than those with N-NO3-, however, treatments with N-NO3- demanded more photosynthetic energy. Part of the ammonium removed in the respective treatments may have been volatilized as a combined effect of the pH increase related to photosynthesis and aeration in the tanks. The results presented here establish the fundamental parameters for the use of U. pseudorotundata as a biofiltering organism for effluents with high concentrations of N-NH4+ and N-NO3-.

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Photosynthetic performance of Chlorella vulgaris R117 mass culture is moderated by diurnal oxygen gradients in an outdoor thin layer cascade

Cited by 17 publications

References 58 publications

Photosynthesis Monitoring in Microalgae Cultures Grown on Municipal Wastewater as a Nutrient Source in Large-Scale Outdoor Bioreactors

Photosynthesis Monitoring in Microalgae Cultures Grown on Municipal Wastewater as a Nutrient Source in Large-Scale Outdoor Bioreactors

Variables Governing Photosynthesis and Growth in Microalgae Mass Cultures

Short-term nutrient removal efficiency by Ulva pseudorotundata (Chlorophyta): potential use for Integrated Multi-Trophic Aquaculture (IMTA)

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