Photosynthetic production of itaconic acid in Synechocystis sp. PCC6803

Chin, Taejun; Sano, Mei; Ohara, Hirotaka; Aso, Yoichi

doi:10.1016/j.jbiotec.2014.12.016

Cited by 27 publications

(13 citation statements)

References 13 publications

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“…PCC6803 [14]. The engineered PCC6802 strain expressing CAD enzyme produced 0.9 mg and 14.5 mg IA L −1 under conditions of constant bubbling with air and 5% CO 2 , respectively.…”

Section: Recombinant Microorganisms For Production Of Itaconic Acidmentioning

confidence: 99%

Emerging biotechnologies for production of itaconic acid and its applications as a platform chemical

Saha¹

2017

Journal of Industrial Microbiology and Biotechnology

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Recently, itaconic acid (IA), an unsaturated C5-dicarboxylic acid, has attracted much attention as a biobased building block chemical. It is produced industrially (>80 g L) from glucose by fermentation with Aspergillus terreus. The titer is low compared with citric acid production (>200 g L). This review summarizes the latest progress on enhancing the yield and productivity of IA production. IA biosynthesis involves the decarboxylation of the TCA cycle intermediate cis-aconitate through the action of cis-aconitate decarboxylase (CAD) enzyme encoded by the CadA gene in A. terreus. A number of recombinant microorganisms have been developed in an effort to overproduce it. IA is used as a monomer for production of superabsorbent polymer, resins, plastics, paints, and synthetic fibers. Its applications as a platform chemical are highlighted. It has a strong potential to replace petroleum-based methylacrylic acid in industry which will create a huge market for IA.

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“…PCC6803 [14]. The engineered PCC6802 strain expressing CAD enzyme produced 0.9 mg and 14.5 mg IA L −1 under conditions of constant bubbling with air and 5% CO 2 , respectively.…”

Section: Recombinant Microorganisms For Production Of Itaconic Acidmentioning

confidence: 99%

Emerging biotechnologies for production of itaconic acid and its applications as a platform chemical

Saha¹

2017

Journal of Industrial Microbiology and Biotechnology

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“…The cadA gene is part of a gene cluster that also comprises a mitochondrial TCA transporter, a membrane permease and a transcription factor proposed to regulate pathway‐specific expression of cluster genes (Li et al ., ). The biochemical elucidation of itaconate production, including its genetic background, has led to a surge of metabolic engineering efforts in recent years with itaconate production established in several heterologous hosts (Kanamasa et al ., ; Li et al ., ; Blumhoff et al ., ; Klement and Büchs, ; Blazeck et al ., ; Huang et al ., ; Okamoto et al ., ; Chin et al ., ; Vuoristo et al ., ).…”

Section: Introductionmentioning

confidence: 99%

Ustilago maydis produces itaconic acid via the unusual intermediate trans‐aconitate

Geiser

Przybilla

Friedrich

et al. 2015

Microbial Biotechnology

114

134

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SummaryItaconic acid is an important biomass‐derived chemical building block but has also recently been identified as a metabolite produced in mammals, which has antimicrobial activity. The biosynthetic pathway of itaconic acid has been elucidated in the ascomycetous fungus A spergillus terreus and in human macrophages. In both organisms itaconic acid is generated by decarboxylation of the tricarboxylic acid (TCA) cycle intermediate cis‐aconitate. Here, we show that the basidiomycetous fungus U stilago maydis uses an alternative pathway and produces itaconic acid via trans‐aconitate, the thermodynamically favoured isomer of cis‐aconitate. We have identified a gene cluster that contains all genes involved in itaconic acid formation. Trans‐aconitate is generated from cis‐aconitate by a cytosolic aconitate‐Δ‐isomerase (Adi1) that belongs to the PrpF family of proteins involved in bacterial propionate degradation. Decarboxylation of trans‐aconitate is catalyzed by a novel enzyme, trans‐aconitate decarboxylase (Tad1). Tad1 displays significant sequence similarity with bacterial 3‐carboxy‐cis,cis‐muconate lactonizing enzymes (CMLE). This suggests that U . maydis has evolved an alternative biosynthetic pathway for itaconate production using the toxic intermediate trans‐aconitate. Overexpression of a pathway‐specific transcription factor (Ria1) or a mitochondrial tricarboxylic acid transporter (Mtt1) resulted in a twofold increase in itaconate yield. Therefore, our findings offer new strategies for biotechnological production of this valuable biomass‐derived chemical.

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“…Chin et al [51] reported the photosynthetic production of IA from CO 2 in a genetically engineered cyanobacterium (Synechocystis sp. PCC6803) by expressing cadA.…”

Section: Bottlenecks and Remediesmentioning

confidence: 99%

New approaches for itaconic acid production: bottlenecks and possible remedies

Bafana

Pandey

2017

Critical Reviews in Biotechnology

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Currently, growing attention is being devoted to the conversion of biomass into value-added products, such as itaconic acid (IA), which is considered as the cleanest alternative to petroleum-based acrylic acid. IA is an unsaturated dicarboxylic acid that is used as a building block chemical for the production of several value-added products such as poly-itaconic acid. IA and its derivatives have a wide range of potential applications in textile, paint, pharmaceutical and chemical industries. Presently, industries are producing IA on the large scale by fermentation from glucose. However, due to the primary utility of glucose as a food, it cannot meet the global demand for IA production in an economical way. The main challenge, so far, has been the production technology, which does not support cost-effective and competitive production of IA. This review discusses the various bottlenecks faced during each step of IA production, along with possible remedies to deal with these problems. Furthermore, it reviews the recent progress in fermentative IA production and sheds light on different microorganisms used, potential substrates and fermentation conditions. The review also covers market potential for IA, which indicates that IA can be produced cost-effectively from sustainable substrates, and it has the potential to replace petrochemicals in the near future.

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Photosynthetic production of itaconic acid in Synechocystis sp. PCC6803

Cited by 27 publications

References 13 publications

Emerging biotechnologies for production of itaconic acid and its applications as a platform chemical

Emerging biotechnologies for production of itaconic acid and its applications as a platform chemical

Ustilago maydis produces itaconic acid via the unusual intermediate trans‐aconitate

New approaches for itaconic acid production: bottlenecks and possible remedies

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