Photosynthetic Proteins in Supported Lipid Bilayers: Towards a Biokleptic Approach for Energy Capture

Wang, Lei; Roth, Johannes S.; Han, Xiaojun; Evans, Stephen D.

doi:10.1002/smll.201403469

Cited by 8 publications

(5 citation statements)

References 201 publications

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“…However, partial (60%) (33) and high physiological orientation (90%) (30) have also been reported, and it has been shown that experimental conditions play a decisive role in determining RC orientation (32,36,42). RC reconstitution has been reported in random orientation in planar lipid bilayers (43)(44)(45)(46) as well, even if high orientation can be also achieved in such systems (42). We have already reported the generation of a transmembrane proton gradient in RCcontaining conventional liposomes (40).…”

mentioning

confidence: 97%

Highly oriented photosynthetic reaction centers generate a proton gradient in synthetic protocells

Altamura

Milano

Tangorra

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

107

105

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Photosynthesis is responsible for the photochemical conversion of light into the chemical energy that fuels the planet Earth. The photochemical core of this process in all photosynthetic organisms is a transmembrane protein called the reaction center. In purple photosynthetic bacteria a simple version of this photoenzyme catalyzes the reduction of a quinone molecule, accompanied by the uptake of two protons from the cytoplasm. This results in the establishment of a proton concentration gradient across the lipid membrane, which can be ultimately harnessed to synthesize ATP. Herein we show that synthetic protocells, based on giant lipid vesicles embedding an oriented population of reaction centers, are capable of generating a photoinduced proton gradient across the membrane. Under continuous illumination, the protocells generate a gradient of 0.061 pH units per min, equivalent to a proton motive force of 3.6 mV⋅min Remarkably, the facile reconstitution of the photosynthetic reaction center in the artificial lipid membrane, obtained by the droplet transfer method, paves the way for the construction of novel and more functional protocells for synthetic biology.

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mentioning

confidence: 97%

Highly oriented photosynthetic reaction centers generate a proton gradient in synthetic protocells

Altamura

Milano

Tangorra

et al. 2017

Proc. Natl. Acad. Sci. U.S.A.

107

105

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“…Planar lipid bilayers on solid supports are one type of model system employed to investigate the biophysical chemistry of cell membranes and simulate biochemical processes occurring at their surface for applications including biosensing, drug discovery, cell mechanobiology and cell culture, and energy capture and storage. − When immobilized on metal surfaces, supported lipid bilayers (SLBs) constitute an attractive platform due to their compatibility with a wide range of surface-sensitive characterization techniques, such as surface plasmon resonance, quartz crystal microbalance, electrochemistry, infrared reflection–absorption spectroscopy, and atomic force microscopy (AFM). − The spontaneous adsorption and rupture of small unilamellar vesicles (SUVs) suspended in aqueous solution onto a solid surface to form a lipid layer, referred to as vesicle fusion, is the most widely used approach for the preparation of SLBs to date . The adsorbed vesicles rupture from the stress or deformation applied on them by vesicle–substrate and vesicle–vesicle interactions .…”

Section: Introductionmentioning

confidence: 99%

Gold-Supported Lipid Membranes Formed by Redox-Triggered Vesicle Fusion on Binary Self-Assembled Monolayers: Ion-Pairing Association and Surface Hydrophilicity

Hmam,

Côté-Dubuc,

Badia

2023

ACS Appl. Mater. Interfaces

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The assembly of biomimetic, planar supported lipid bilayers (SLBs) by the popular vesicle fusion method, which relies on the spontaneous adsorption and rupture of small unilamellar vesicles from aqueous solution on a solid surface, typically works with a limited range of support materials and lipid systems. We previously reported a conceptual advance in the formation of SLBs from vesicles in the gel or fluid phase using the interfacial ion-pairing association of charged phospholipid headgroups with electrochemically generated cationic ferroceniums bound to a self-assembled monolayer (SAM) chemisorbed to gold. This redox-driven approach lays down a single bilayer membrane on the SAM-modified gold surface at room temperature within minutes and is compatible with both anionic and zwitterionic phospholipids. The present work explores the effects of the surface ferrocene concentration and hydrophobicity/hydrophilicity on the formation of continuous SLBs of dialkyl phosphatidylserine, dialkyl phosphatidylglycerol, and dialkyl phosphatidylcholine using binary SAMs of ferrocenylundecanethiolate (FcC11S) and dodecanethiolate (CH3C11S) or hydroxylundecanethiolate (HOC11S) comprising different surface mole fractions of ferrocene (χFc surf). An increase in the surface hydrophilicity and surface free energy of the FcC11S/HOC11S SAM mitigates the decrease in the attractive ion-pairing interactions resulting from a reduced χFc surf. SLBs of ≳80% area coverage form on the FcC11S/HOC11S SAM for all the phospholipid types down to χFc surf of at least 0.2, composition yielding a water contact angle (θW) of 44 ± 4°. By contrast, a greater number of ion-pairing interactions is required on the hydrophobic FcC11S/CH3C11S surface to drive the vesicle fusion process; bilayers or bilayer patches form at χFc surf ≳ 0.6 (θW = 97 ± 3°). These findings will aid in tailoring the surface chemistry of redox-active modified surfaces to widen the conditions that yield supported lipid membranes.

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“…Recently, atomic force microscopy (AFM) observations of thylakoid membranes adsorbed on solid supports have revealed details of the molecular organization within the membrane. − Furthermore, purified components of photosynthetic machinery (LH2 and LH1-reaction center complexes isolated from purple bacteria, or solo-LHCII isolated from land plants, etc. ) have been reconstituted in a substrate-supported model membrane to study their lateral organization, − clustering, , membrane stacking, − and potential as nanomaterials . Supported membrane systems are amenable to highly sensitive interfacial analyses (including AFM), and they can be generated in a designed pattern, − which greatly enhances their utility in analyzing the photosynthetic reactions.…”

Section: Introductionmentioning

confidence: 99%

Photosynthetic Model Membranes of Natural Plant Thylakoid Embedded in a Patterned Polymeric Lipid Bilayer

et al. 2020

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Thylakoid membranes in the chloroplast of plants, algae, and cyanobacteria are the powerhouse of photosynthesis, capturing solar energy and converting it into chemical energy. Although their structures and functions have been extensively studied, the intrinsically heterogeneous and dynamic nature of the membrane structures is still not fully understood. Investigating native thylakoid membranes in vivo is difficult due to their small size and limited external access to the chloroplast interior, while the bottom-up approaches based on model systems have been hampered by the sheer complexity of the native membrane. Here, we try to fill the gap by reconstituting the whole thylakoid membrane into a patterned substrate-supported planer bilayer. A mixture of thylakoid membrane purified from spinach leaves and synthetic phospholipid 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) vesicles spontaneously formed a laterally continuous and fluid two-dimensional (2D) membrane in the scaffold of the patterned polymeric bilayer. Chlorophyll fluorescence arising from photosystem II (PSII) recovered after photobleaching, suggesting that the membrane components are laterally mobile. The reversible changes of chlorophyll fluorescence in the presence of the electron acceptors and/or inhibitors indicated that the electron transfer activity of PSII was retained. Furthermore, we confirmed the electron transfer activity of photosystem I (PSI) by observing the generation of nicotinamide adenine dinucleotide phosphate (NADPH) in the presence of water-soluble ferredoxin and ferredoxin−NADP + reductase. The lateral mobility of membrane-bound molecules and the functional reconstitution of major photosystems provide evidence that our hybrid thylakoid membranes could be an excellent experimental platform to study the 2D molecular organization and machinery of photosynthesis.

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Photosynthetic Proteins in Supported Lipid Bilayers: Towards a Biokleptic Approach for Energy Capture

Cited by 8 publications

References 201 publications

Highly oriented photosynthetic reaction centers generate a proton gradient in synthetic protocells

Highly oriented photosynthetic reaction centers generate a proton gradient in synthetic protocells

Gold-Supported Lipid Membranes Formed by Redox-Triggered Vesicle Fusion on Binary Self-Assembled Monolayers: Ion-Pairing Association and Surface Hydrophilicity

Photosynthetic Model Membranes of Natural Plant Thylakoid Embedded in a Patterned Polymeric Lipid Bilayer

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